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Anti-α-Actinin (Sarcomeric) antibody, Mouse monoclonal

Name: Anti-α-Actinin (Sarcomeric) antibody, Mouse monoclonal
Brand: SIGMA
Price: 801 CAD

clone EA-53, purified from hybridoma cell culture

Synonym(s):

Anti-ACTN2, Anti-Actinin α2, Anti-CMD1AA, Anti-MGC107582, Anti-1110008F24Rik, Sarcomeric Alpha Actinin Antibody, Sarcomeric Alpha Actinin Antibody - Anti-α-Actinin (Sarcomeric) antibody, Mouse monoclonal

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Pack Size	SKU	Availability	Price
100 μL		Please contact Customer Service for Availability	$801.00
200 μL		Please contact Customer Service for Availability	$1,000.00

About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Conjugate:

unconjugated

Clone:

EA-53, monoclonal

Application:

IHC (p), WB

Citations:

125

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Properties

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

EA-53, monoclonal

form

buffered aqueous solution

species reactivity

cat, rat, fish, rabbit, sheep, bovine, goat, canine, pig, mouse, human, hamster, snake, frog, lizard, chicken

concentration

~1.0 mg/mL

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 10-20 μg/mL using protease-digested sections of human tongue., western blot: 2-4 μg/mL using rat skeletal muscle extract

isotype

IgG1

UniProt accession no.

Q08043

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ACTN2(88)
mouse ... Actn2(11472)
rabbit ... ACTN3(100009544)
rat ... Actn2(291245)

Description

General description

α-Actinin is a 100kDa actin-binding protein that is found in muscle as well as non-muscle cells. In smooth muscles, α-actinin is present in dense bodies and plaques whereas in normal skeletal muscles, it is associated with z-discs that define muscle sarcomeres. The gene encoding α-actinin is mapped to the human chromosome 11q13.2. α-Actinin belongs to the spectrin protein superfamily.
α-Actinin dimerizes in an antiparallel fashion via interaction of the central rod domains. Four isoforms are found in human and mice. α-actinin-1 is ubiquitously expressed and located primarily in focal adhesions, whereas α-actinin-4 is present in certain membrane ruffles and seems to play a role in endocytosis and tumor cell motility.
ACTN1 (α-actinin-1) and ACTN4 (α-actinin-4) encodes non-muscle proteins, while ACTN2 (α-actinin-2) and ACTN3 (α-actinin-3) encodes muscle proteins.
Monoclonal Anti-α-Actinin (Sarcomeric) shows wide reactivity, as it binds to human, bovine, pig, sheep, rabbit, goat, hamster, cat, rat, mouse, dog, chicken, lizard, snake, frog and fish α-actinin.

Immunogen

Purified rabbit skeletal α actinin.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)
Immunofluorescence (2 papers)
Immunohistochemistry (1 paper)

Mouse Monoclonal Anti-alpha-Actinin (Sarcomeric) antibody has been used for immunocytochemical (1:800) and immunohistochemical (1:100) applications.

Western blot analysis of transfected HEK-293 cells were performed to evaluate actinin expression using monoclonal mouse anti-actinin antibody.

Biochem/physiol Actions

α-Actinin maintains the structure of cytoskeletal organization and regulates muscle contraction. α-Actinin functions as an actin crosslinker and promotes cell migration. Mutations in the α-actinin gene is observed in heterogeneous hypertrophic cardiomyopathy and in juvenile onset atrial fibrillation.

Monoclonal Anti-α-Actinin (Sarcomeric) specifically recognizes α-skeletal muscle actinin and α-cardiac muscle actinin.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

For continuous use, store at 2-8 °C for up to one month.
For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Show Differences

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This Item	A7811	SAB4200689	A5316
Sigma-Aldrich A7732 Anti-α-Actinin (Sarcomeric) antibody, Mouse monoclonal	Sigma-Aldrich A7811 Monoclonal Anti-α-Actinin (Sarcomeric) antibody produced in mouse	Sigma-Aldrich SAB4200689 Anti-Actin (α-Sarcomeric) antibody, Mouse monoclonal	Sigma-Aldrich A5316 Monoclonal Anti-β-Actin antibody produced in mouse
conjugate unconjugated	conjugate unconjugated	conjugate -	conjugate unconjugated
Gene Information human ... ACTN2(88) mouse ... Actn2(11472) rabbit ... ACTN3(100009544) rat ... Actn2(291245)	Gene Information human ... ACTN2(88) mouse ... Actn2(11472) rat ... Actn2(291245)	Gene Information human ... ACTA2(59)	Gene Information human ... ACTB(60) mouse ... Actb(11461) rat ... Actb(81822)
species reactivity cat, rat, fish, rabbit, sheep, bovine, goat, canine, pig, mouse, human, hamster, snake, frog, lizard, chicken	species reactivity mouse, human, frog, pig, feline, chicken, hamster, canine, bovine, fish, snake, rabbit, sheep, goat, rat, lizard	species reactivity rat, chicken, bovine, mouse, guinea pig, rabbit, human, planaria(flatworm), fish, Xenopus	species reactivity rabbit, sheep, cat, guinea pig, bovine, mouse, chicken, wide range, Hirudo medicinalis, canine, Drosophila, carp, pig, rat, human
antibody form purified immunoglobulin	antibody form ascites fluid	antibody form purified immunoglobulin	antibody form ascites fluid
clone EA-53, monoclonal	clone EA-53, monoclonal	clone 5C5, monoclonal	clone AC-74, monoclonal
biological source mouse	biological source mouse	biological source mouse	biological source mouse

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

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Certificates of Analysis (COA)

Lot/Batch Number

0000534831

0000524212

0000457166

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Global Trade Item Number

SKU	GTIN
A7732-200UL	04061833386224
A7732-100UL	04061837718946

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