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A7827

Alkaline Phosphatase-Anti-Alkaline Phosphatase antibody produced in mouse

clone AP1B9, purified immunoglobulin, buffered aqueous solution

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Synonym(s):

APAAP

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1 mL
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$449.00

About This Item

NACRES:
NA.46
UNSPSC Code:
12352203
Clone:
AP1B9, monoclonal
Technique(s):
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40 using human tonsil
Application:
IHC (p)
Citations:
7

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biological source

mouse

Quality Segment

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

AP1B9, monoclonal

description

Concentrate

form

buffered aqueous solution

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40 using human tonsil

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

General description

A soluble complex of calf intestinal alkaline phosphatase and mouse monoclonal antibodies to alkaline phosphatase. The product is a soluble complex of calf intestinal alkaline phosphatase and mouse monoclonal antibodies to alkaline phosphatase.
Monoclonal Anti-Alkaline Phosphatase (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Application

Alkaline Phosphatase-Anti-Alkaline Phosphatase antibody produced in mouse has been used in:
  • Immunohistological analysis
  • Immunohistochemistry assay
  • Immunocytochemical staining

Apoptosis of β-cells in mouse pancreatic sections was analyzed by incubating section with TUNEL and anti-insulin antibody. APAAP was used as the secondary antibody and BCIP/NBT (Sigma) as the substrate.

Biochem/physiol Actions

Mouse Monoclonal Alkaline Phosphatase Anti-Alkaline Phosphatase is a soluble complex formed from levamisole-resistant calf intestinal alkaline phosphatase and purified mouse monoclonal antibody to alkaline phosphatase. The use of a mouse monoclonal alkaline phosphatase anti-alkaline phosphatase (APAAP) complex, in conjunction with a primary mouse antibody and a secondary bridging antibody (anti-mouse IgG), results in an intense signal with a very low background, while avoiding problems inherent to the covalent conjugation of antibodies. The technology of unlabelled antibody peroxidase-anti peroxidase (PAP) and the alkaline phosphatase anti- alkaline phosphatase (APAAP) method has applications in immunostaining. APAAP monoclonal antibodies complex are homogenous and this method is effective and its sensitive.

Physical form

Solution in 0.05 M Tris buffered saline, pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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A3687A7434A1682
description

clone AP1B9, purified immunoglobulin, buffered aqueous solution

description

affinity isolated antibody, buffered aqueous glycerol solution

description

affinity isolated antibody, buffered aqueous glycerol solution

description

affinity isolated antibody, buffered aqueous solution

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

clone

AP1B9, monoclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

biological source

mouse

biological source

goat

biological source

goat

biological source

goat

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

form

buffered aqueous solution

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

buffered aqueous solution

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40 using human tonsil

technique(s)

direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000

technique(s)

western blot (chemiluminescent): 1:30,000 using β-actin in total cell extract of HiLa cells (5-10 μg per well), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20, direct ELISA: 1:40,000

technique(s)

western blot: 1:200,000-1:400.000 using assay detecting β-Actin in total cell extract of HeLa cells (5-10 μg per well), direct ELISA: 1:40,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:10


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Storage Class

10 - Combustible liquids

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable



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