Yes, following reconstitution, this material may be stored at -20°C, in aliquots, for long term use. Avoid repeated freeze/thaw cycles. Prepared solutions may also be stored refrigerated for short term use. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/598/704/a8273dat.pdf
A8273
Anti-α-Amylase antibody produced in rabbit
fractionated antiserum, lyophilized powder
Anti-α-Amylase antibody produced in rabbit
Synonym(s):
Alpha Amylase Antibody, Alpha Amylase Antibody - Anti-α-Amylase antibody produced in rabbit
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| Size/SKU | Availability | Price |
|---|---|---|
1 vial | Available to ship TODAYfromMILWAUKEE | $712.00 |
About This Item
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ELISA (i), ODD
Citations:
111
biological source
rabbit
Quality Segment
conjugate
unconjugated
antibody form
fractionated antiserum
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized powder
species reactivity
human
packaging
vial of 2 mL lyophilized antiserum
technique(s)
Ouchterlony double diffusion: suitable, indirect ELISA: 1:4,000-1:6,000
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... AMY1A(276)
General description
α-amylase is primarily produced in abundance in the salivary glands and pancreas. The expression is also seen in jejunum and mammary glands. The genes encoding α-amylase are AMY1 and AMY2 located in the short arm of chromosome 1. AMY1 is responsible for producing the enzyme in saliva and mammary gland and AMY2 produces the enzyme synthesized in the pancreas. The expression of AMY1 occurs also in certain tumour tissues. Mammalian amylases are composed of three structural domains (A, B and C). Domain A contains the active site with two aspartate and one glutamate residue. Domain B borders the active site region and is essential for maintaining the protein conformation. Domain C is involved in catalytic mechanism.
Immunogen
human salivary α-amylase.
Application
Anti-α-Amylase antibody produced in rabbit has also been used in immunocytochemistry analysis.
Anti-a-Amylase antibody produced in rabbit has been used in immunofluorescence and immunohistochemical analysis.
Mouse pancreatic sections were fixed in 4% paraformaldehyde and used for immunohistochemistry using rabbit anti-amylase antibody at a dilution of 1:100.
Biochem/physiol Actions
α-Amylase is essential for catalyzing the primary step in starch digestion, a main source of carbohydrate in the human diet. α-amylase hydrolysis the polysaccharide with the ultimate production of maltose, maltotriose and limit dextrins as the main products.
The antiserum is specific for human a-amylase found in human saliva and human pancreatic extract. No reaction with other human saliva proteins or pancreatic extract proteins is observed.
Physical form
Lyophilized from 0.01 M phosphate buffered saline, pH 7.2
Preparation Note
Reconstitute with 2 mL deionized water.
1 of 1
This Item | |||
|---|---|---|---|
| description fractionated antiserum, lyophilized powder | description clone AMY-7, purified from hybridoma cell culture | description recombinant, expressed in HEK 293 cells | description whole antiserum |
| biological source rabbit | biological source mouse | biological source rabbit | biological source rabbit |
| antibody form fractionated antiserum | antibody form purified immunoglobulin | antibody form purified antibody | antibody form whole antiserum |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| conjugate unconjugated | conjugate - | conjugate unconjugated | conjugate unconjugated |
| form lyophilized powder | form buffered aqueous solution | form lyophilized | form - |
| clone polyclonal | clone AMY-7, monoclonal | clone 1G14, recombinant monoclonal | clone polyclonal |
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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A8273を水２ｍｌで溶解した後の保存法を教えてください。-20度でしょうか。
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