Skip to Content
MilliporeSigma

Key Documents

View All Documentation

Skip To

A8656

Alcohol Dehydrogenase from Saccharomyces cerevisiae

Synonym(s):

ADH, Alcohol Dehydrogenase from yeast, Alcohol:NAD+ oxidoreductase

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View
Pack SizeSKUAvailabilityPrice
1 vial
Check Cart for Availability
$144.00

About This Item

CAS Number:
9031-72-5
UNSPSC Code:
12352204
NACRES:
NA.25
EC Number:
232-870-4
MDL number:
MFCD00081305
EC Number:
1.1.1.1 (BRENDA, IUBMB)
Biological source:
Saccharomyces cerevisiae

$144.00

Check Cart for Availability
​
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

biological source

Saccharomyces cerevisiae

Quality Level

200

form

powder

mol wt

~150,000

packaging

vial of 25 mg

storage temp.

−20°C

Application

Alcohol Dehydrogenase from Saccharomyces cerevisiae has been used as a gel filtration molecular weight marker/ It has also been used as a component of nine protein mixture for mass spectroscopy analysis.

Biochem/physiol Actions

ADH (alcohol dehydrogenase) is one of the first enzymes to be isolated and purified. NAD+ is its coenzyme. Three isozymes of yeast ADH, that is, yeast alcohol dehydrogenase-1, 2 and 3 (YADH-1, -2, -3) have been identified. YADH-1 is expressed during anaerobic fermentation, YADH-2 is expressed in the cytoplasm and YADH-3 is localized to the mitochondria. A 141kDa tetramer containing 4 equal subunits. The active site of each subunit contains a zinc atom. Each active site also contains 2 reactive sulfhydryl groups and a histidine residue.

Isoelectric point: 5.4-5.8

Optimal pH: 8.6-9.0

Substrates: Yeast ADH is most active with ethanol and its activity decreases as the size of the alcohol increases or decreases. Branched chain alcohols and secondary alcohols also have very low activity.

KM (ethanol) = 2.1 × 10-2 M
KM (methanol = 1.3 × 10-1 M
KM (isopropanol) = 1.4 × 10-1 M

Inhibitors: Compounds that react with free sulfhydryls, including N-alkylmaleimides and iodoacetamide.
Zinc chelator inhibitors, including 1,10-phenanthroline,
8-hydroxyquinoline, 2,2′-dipyridyl, and thiourea.
Substrate analogue inhibitors, including β-NAD analogs, purine and pyrimidine derivatives, chloroethanol, and fluoroethanol.

Extinction Coefficient: E1% = 14.6 (water, 280 nm)
Alcohol Dehydrogenase (ADH) is an oxidoreductase and also a pyridine nucleotide-dependent dehydrogenase. It catalyzes the generation of aldehydes or ketones by reversible oxidation of alcohols. ADH in parallel also mediates the reduction of the nicotinamide adenine dinucleotide (NAD+) or nicotinamide adenine dinucleotide phosphate (NADP+). ADH from yeast is more active than mammalian ADHs.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
A701174931A3263
Alcohol Dehydrogenase from Saccharomyces cerevisiae

Sigma-Aldrich

A8656

Alcohol Dehydrogenase from Saccharomyces cerevisiae

Alcohol Dehydrogenase from Saccharomyces cerevisiae ≥300 units/mg protein, lyophilized powder (contains buffer salts), Mw 141-151 kDa

Sigma-Aldrich

A7011

Alcohol Dehydrogenase from Saccharomyces cerevisiae

Alcohol Dehydrogenase ≥10.0 U/mg

Sigma-Aldrich

74931

Alcohol Dehydrogenase

Alcohol Dehydrogenase from Saccharomyces cerevisiae powder, ≥300 units/mg protein, mol wt ~141,000 (four subunits)

Sigma-Aldrich

A3263

Alcohol Dehydrogenase from Saccharomyces cerevisiae

biological source

Saccharomyces cerevisiae

biological source

Saccharomyces cerevisiae

biological source

-

biological source

bakers yeast

form

powder

form

lyophilized powder (contains buffer salts)

form

powder

form

powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

mol wt

~150,000

mol wt

Mw 141-151 kDa

mol wt

-

mol wt

~141,000 (four subunits)

Quality Level

200

Quality Level

300

Quality Level

-

Quality Level

200

packaging

vial of 25 mg

packaging

-

packaging

-

packaging

-

Still not finding the right product?

Explore all of our products under Alcohol Dehydrogenase from Saccharomyces cerevisiae

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000487135
0000487135
0000327010
0000327010
0000327008

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Protocols

Gel Filtration Markers Kit for Protein Molecular Weights 12,000-200,000 Da

Gel filtration chromatography is an established method for determining the size and molecular mass of proteins.

Enzymatic Assay of Alcohol Dehydrogenase (EC 1.1.1.1)

To measure alcohol dehydrogenase activity, this assay uses β-nicotinamide adenine dinucleotide phosphate and a continuous spectrophotometric rate determination at 340 nm.

Yeast alcohol dehydrogenase structure and catalysis
Raj S, et al.
Biochemistry, 53(36), 5791-5803 (2014)
Determination of hydrodynamic radius of proteins by size exclusion chromatography
La Verde V, et al.
Bio-protocol, 7(8), 1-14 (2017)
Study of Reduction Properties of Enzyme Alcohol Dehydrogenase from Saccharomyces cerevisiae Meyen ex. Hansen on Some Selected Compounds
Khan SYN
International journal of language & communication disorders, 3(4), 1218-1222 (2017)
View All Related Papers

Global Trade Item Number

SKUGTIN
A8656-1VL04061833400463

Questions

Reviews

No rating value

Active Filters