We make no claims that this antibody will detect the soluble C-terminal fragments CTFγ, CTFα, and CTFβ. We know this antibody will detect APP695, APP751 and APP770. The following references (Neurobiol Aging. 2013 Jun; 34(6): 1680–1686 & J Alzheimers Dis. 2010; 22(2): 683–984) were able to show that this antibody can detect CTFα or APP-C83.
A8717
Anti-Amyloid Precursor Protein (APP) Antibody
Enhanced Validation
rabbit polyclonal
Synonym(s):
APP antibody, Anti-APP
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| Size/SKU | Availability | Price |
|---|---|---|
25 μL | Available to ship TODAYfromMILWAUKEE | $192.00 |
100 μL | Available to ship TODAYfromMILWAUKEE | $552.00 |
0.2 mL | Available to ship TODAYfromMILWAUKEE | $763.00 |
About This Item
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ARR, IHC (p), WB
Citations:
363
$192.00
Available to ship TODAYDetails
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Product Name
Anti-Amyloid Precursor Protein, C-Terminal antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
biological source
rabbit
Quality Segment
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 95-100 kDa
species reactivity
mouse, human, rat
packaging
antibody small pack of 25 μL
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1000 using formic acid-treated sections of human Alzheimer′s disease (AD) brain, microarray: suitable, western blot: 1:4,000 using rat brain extract
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... APP(351)
mouse ... App(11820)
rat ... App(54226)
General description
Amyloid precursor proteins (APPs) are transmembrane glycoproteins that are found in a wide range of tissues. APP is expressed in the brain. It is located on human chromosome 21. APPs have 3 main isoforms, namely, APP695, APP751 and APP770 that are derived from alternative splicing events in cells. Accumulation of the cleavage products of APP, such as the β-amyloid peptide, can cause Alzheimer′s disease.
The immunogen sequence is identical to rat and mouse APP695 and in the APP isoforms APP751 and APP770. The antibody recognizes APP695, APP751 and APP770.
Immunogen
Synthetic peptide corresponding to the C-terminal of human APP695 conjugated to KLH.
Application
Rabbit Anti-Amyloid Precursor Protein, C-Terminal antibody has been used for immunoprecipitation, immunohistochemical analysis, immunohistochemical staining and western blot applications. The product can also be used for IHC (1:1,000) and microarray studies.
SH-SY5Y cell lysates were analyzed by western blot using rabbit anti-Amyloid Precursor Protein, C-Terminal as the primary antibody at a 1:500 dilution.
Biochem/physiol Actions
In transiently transfected cell lines, APP (amyloid precursor protein) regulates cell growth, motility, neurite outgrowth and cell survival. The intracellular C-terminus of APP acts as a transcriptional regulator and its amino acid domain serves as a regulator of its own intracellular sorting. Mutation of the gene leads to cerebral amyloid angiopathy. Accumulation of the cleavage products of APP, such as the β-amyloid peptide can cause Alzheimer′s disease.
Physical form
Supplied in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative.
Preparation Note
For continuous use, store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing, or storage in "frostfree" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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This Item | |||
|---|---|---|---|
| description IgG fraction of antiserum, buffered aqueous solution | description IgG fraction of antiserum, buffered aqueous solution | description clone NAB 228, purified from hybridoma cell culture | description affinity isolated antibody, buffered aqueous solution |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| biological source rabbit | biological source rabbit | biological source mouse | biological source rabbit |
| antibody form IgG fraction of antiserum | antibody form IgG fraction of antiserum | antibody form purified immunoglobulin | antibody form affinity isolated antibody |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1000 using formic acid-treated sections of human Alzheimer′s disease (AD) brain, western blot: 1:4,000 using rat brain extract, microarray: suitable | technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using formic acid-treated sections of human Alzheimer′s disease (AD) brain, microarray: suitable, western blot: 1:1,000 using rat brain extract or supernatant of 293T cells secreting APP. | technique(s) immunohistochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, microarray: suitable, western blot: 2-4 μg/mL using cell extract of the human embryonal carcinoma NTERA-2 (NT2/D1) cells, treated for 2-3 weeks with 10 μM retinoic acid | technique(s) dot blot: 0.05-1 μg/mL, immunoblotting: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: 1:200, radioimmunoassay: 1:200 |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
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Storage Class
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Which C-terminal fragment(s) of APP does this antibody recognize? I am primarily interested in detecting the alpha CTF (C83).
1 answer-
Helpful?
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Does this antibody react with Amyloid Beta
1 answer-
No, this antibody does not cross react with Amyloid beta peptide. This product is expected to cross react with all isoforms of APP. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/164/132/a8717dat.pdfHelpful?
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