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Anti-Amyloid Precursor Protein (APP) Antibody

Name: Anti-Amyloid Precursor Protein (APP) Antibody
Brand: SIGMA
Price: 509.15 EUR

rabbit polyclonal

Synonym(s):

Anti-APP

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Size/SKU	Availability	Price
0.2 mL	Please contact Customer Service for Availability	€599.00 €509.15

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD02095830

Conjugate:

unconjugated

Clone:

polyclonal

Application:

ARR, IHC (p), WB

Citations:

€509.15

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Properties

Product Name

Anti-Amyloid Precursor Protein, N-Terminal antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 95-100 kDa

species reactivity

human, rat, mouse

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using formic acid-treated sections of human Alzheimer′s disease (AD) brain, microarray: suitable, western blot: 1:1,000 using rat brain extract or supernatant of 293T cells secreting APP.

UniProt accession no.

P05067

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... APP(351)
mouse ... App(11820)
rat ... App(54226)

Description

General description

Amyloid precursor proteins (APPs) are transmembrane glycoproteins that are found in a wide range of tissues. APPs have 3 main isoforms, namely, APP695, APP751 and APP770 that are derived from alternative splicing events in cells. It is expressed at high levels in the brain. APP gene is mapped to human chromosome 21q11.2-q21. It is a 695 amino acid protein which possesses a large ectodomain and comparatively short intracellular region.

The immunogen sequence is identical to the APP isoforms APP₇₅₁ and APP₇₇₀ and is highly conserved (single amino acid substitution) in rat and mouse APP₆₉₅. The antibody recognizes APP₆₉₅, APP₇₅₁ and APP₇₇₀.

Immunogen

synthetic peptide corresponding to the N-terminal of human APP₆₉₅ (amino acids 46-60) conjugated to KLH.

Application

Anti-Amyloid Precursor Protein, N-Terminal antibody produced in rabbit has been used in:

western blotting
immunostaining
immunofluorescence

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

SH-SY5Y cell lysates were analyzed by western blot using rabbit anti-Amyloid Precursor Protein, C-Terminal as the primary antibody at a 1:500 dilution.

Biochem/physiol Actions

Amyloid precursor proteins (APPs) regulates cell growth, motility, neurite outgrowth and cell survival. The intracellular C-terminus of APP serves as a transcriptional regulator and as a receptor for kinesin-1-mediated axonal transport. Alzheimer′s disease is characterized by deposition of amyloid in the central nervous system, in neurite plaques and on cerebral vasculature. Mutations in the APP gene are linked with rare forms of autosomal dominant familial Alzheimer′s Disease (FAD). APPs undergo post-translational processing including N- and O-glycosylation, phosphorylation and sulfation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	A8354	A3981	A1976
$Anti-Amyloid Precursor Protein, N-Terminal antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich A8967 Anti-Amyloid Precursor Protein, N-Terminal antibody produced in rabbit	Sigma-Aldrich A8354 Anti-β-Amyloid antibody, Mouse monoclonal	Sigma-Aldrich A3981 Anti-β-Amyloid antibody, Mouse monoclonal	Sigma-Aldrich A1976 Anti-Amyloid Peptide β, Cleavage Site 42 antibody produced in rabbit
description IgG fraction of antiserum, buffered aqueous solution	description clone NAB 228, purified from hybridoma cell culture	description clone BAM-10, purified from hybridoma cell culture	description affinity isolated antibody, buffered aqueous solution
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
antibody form IgG fraction of antiserum	antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form affinity isolated antibody
biological source rabbit	biological source mouse	biological source mouse	biological source rabbit
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 100
shipped in dry ice	shipped in dry ice	shipped in dry ice	shipped in dry ice
technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using formic acid-treated sections of human Alzheimer′s disease (AD) brain, western blot: 1:1,000 using rat brain extract or supernatant of 293T cells secreting APP., microarray: suitable	technique(s) immunohistochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, microarray: suitable, western blot: 2-4 μg/mL using cell extract of the human embryonal carcinoma NTERA-2 (NT2/D1) cells, treated for 2-3 weeks with 10 μM retinoic acid	technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2.5-5 μg/mL using formic-acid treated tissue sections of human Alzheimer disease brain tissue, indirect ELISA: suitable, western blot: suitable	technique(s) dot blot: 0.05-1 μg/mL, immunoblotting: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: 1:200, radioimmunoassay: 1:200

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c