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B0287

Monoclonal Anti-FITC−Biotin antibody produced in mouse

clone FL-D6, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Monoclonal Anti-FITC

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0.2 mL

Estimated to ship TODAYfromMILWAUKEE

$568.00
$482.80

About This Item

NACRES:
NA.43
UNSPSC Code:
12352203
Conjugate:
biotin conjugate
Clone:
FL-D6, monoclonal
Application:
IHC (p)
Citations:
8

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biological source

mouse

Quality Segment

conjugate

biotin conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

FL-D6, monoclonal

form

buffered aqueous solution

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400 using human tonsil

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Monoclonal Anti-FITC (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Immunogen

FITC-BSA conjugate

Application

Monoclonal Anti-FITC-Biotin antibody produced in mouse has been used:
  • for amplification of signal in immunofluorescence assays
  • in Ag-activated beads for flow-based micro immunoassay of parathyroid hormone and IL-5
  • enzyme linked immunosorbent assay (ELISA)

Biochem/physiol Actions

Fluorochrome labeling provides a rapid and accurate localization of the site of antigen-antibody interaction when one of the reactants from parts of a cell, tissue or other biological structure. Fluorescein isothiocyanate (FITC) is a commonly used marker for antibodies in immunofluorescence techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labelled substances. FITC is widely used as a hapten to label different proteins and anti-FITC antibodies are used to identify these labeled proteins. Antibodies to FITC serve as universal indicator reagents by bridging FITC with another immunohistochemical reagent, such as alkaline phosphatase, horseradish peroxidase or biotin.
The antibody reacts with both free and conjugated fluorescein isothiocyanate (FITC).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Monoclonal Anti-FITC−Biotin antibody produced in mouse clone FL-D6, purified immunoglobulin, buffered aqueous solution

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Monoclonal Anti-Biotin−Peroxidase antibody produced in mouse clone BN-34, purified immunoglobulin

Sigma-Aldrich

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Monoclonal Anti-Biotin−Peroxidase antibody produced in mouse

description

clone FL-D6, purified immunoglobulin, buffered aqueous solution

description

clone DI-22, purified from hybridoma cell culture

description

~1 mg/mL, clone CY-96, purified immunoglobulin, buffered aqueous solution

description

clone BN-34, purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

clone

FL-D6, monoclonal

clone

DI-22, monoclonal

clone

CY-96, monoclonal

clone

BN-34, monoclonal

conjugate

biotin conjugate

conjugate

biotin conjugate

conjugate

biotin conjugate

conjugate

peroxidase conjugate

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

isotype

IgG1

isotype

IgG1

isotype

IgG2a

isotype

IgG1

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

-

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Storage Class

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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