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B0287

Monoclonal Anti-FITC−Biotin antibody produced in mouse

clone FL-D6, purified immunoglobulin, buffered aqueous solution

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Synonym(s):

Monoclonal Anti-FITC

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0.2 mL

Estimated to ship TODAYfromMILWAUKEE

$589.00

About This Item

NACRES:
NA.43
UNSPSC Code:
12352203
Conjugate:
biotin conjugate
Clone:
FL-D6, monoclonal
Application:
IHC (p)
Citations:
8

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biological source

mouse

Quality Segment

conjugate

biotin conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

FL-D6, monoclonal

form

buffered aqueous solution

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400 using human tonsil

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Monoclonal Anti-FITC (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Immunogen

FITC-BSA conjugate

Application

Monoclonal Anti-FITC-Biotin antibody produced in mouse has been used:
  • for amplification of signal in immunofluorescence assays
  • in Ag-activated beads for flow-based micro immunoassay of parathyroid hormone and IL-5
  • enzyme linked immunosorbent assay (ELISA)

Biochem/physiol Actions

Fluorochrome labeling provides a rapid and accurate localization of the site of antigen-antibody interaction when one of the reactants from parts of a cell, tissue or other biological structure. Fluorescein isothiocyanate (FITC) is a commonly used marker for antibodies in immunofluorescence techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labelled substances. FITC is widely used as a hapten to label different proteins and anti-FITC antibodies are used to identify these labeled proteins. Antibodies to FITC serve as universal indicator reagents by bridging FITC with another immunohistochemical reagent, such as alkaline phosphatase, horseradish peroxidase or biotin.
The antibody reacts with both free and conjugated fluorescein isothiocyanate (FITC).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Monoclonal Anti-FITC−Biotin antibody produced in mouse clone FL-D6, purified immunoglobulin, buffered aqueous solution

Sigma-Aldrich

B0287

Monoclonal Anti-FITC−Biotin antibody produced in mouse

Anti-Biotin−FITC antibody produced in goat affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

F6762

Anti-Biotin−FITC antibody produced in goat

Anti-Mouse IgG (Fc specific) F(ab′)2 fragment−FITC antibody produced in goat affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

F8646

Anti-Mouse IgG (Fc specific) F(ab′)2 fragment−FITC antibody produced in goat

Anti-Human IgG (γ-chain specific), F(ab′)2 fragment–FITC antibody produced in goat affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

F1641

Anti-Human IgG (γ-chain specific), F(ab′)2 fragment–FITC antibody produced in goat

description

clone FL-D6, purified immunoglobulin, buffered aqueous solution

description

affinity isolated antibody, buffered aqueous solution

description

affinity isolated antibody, buffered aqueous solution

description

affinity isolated antibody, buffered aqueous solution

conjugate

biotin conjugate

conjugate

FITC conjugate

conjugate

FITC conjugate

conjugate

FITC conjugate

clone

FL-D6, monoclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

biological source

mouse

biological source

goat

biological source

goat

biological source

goat

shipped in

dry ice

shipped in

-

shipped in

-

shipped in

-

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

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Storage Class

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000122931
    077K4828
    070M4830
    069H4882
    039M4869V

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