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B1531

Monoclonal Anti-Phosphotyrosine−Biotin antibody produced in mouse

clone PT-66, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Monoclonal Anti-Phosphotyrosine, Phospho-Tyr, Phospho-tyrosine, p-Tyr

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Size/SKUAvailabilityPrice
0.2 mL
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$661.00

About This Item

NACRES:
NA.44
UNSPSC Code:
12352203
Conjugate:
biotin conjugate
Clone:
PT-66, monoclonal
Application:
DB, ELISA (d)
Citations:
5

$661.00

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biological source

mouse

Quality Segment

conjugate

biotin conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

PT-66, monoclonal

form

buffered aqueous solution

technique(s)

direct ELISA: 1:50,000, dot blot: 1:32,000

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

As determined by ELISA and competitive ELISA, the antibody reacts specifically with phosphorylated tyrosine, both as free amino acid or conjugated to carriers such as BSA or KLH. No cross-reactivity is observed with non-phosphorylated tyrosine, phosphothreonine, phosphoserine, AMP or ATP.
Monoclonal Anti-Phosphotyrosine (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
Reversible phosphorylation of proteins is an important post-translational modification that plays a regulatory role in the expression of most proteins in the cells. Reversible phosphorylation at multiple serine, tyrosine and threonine residues mediates numerous signalling pathways in both prokaryotic and eukaryotic cells . Cellular proteins with phosphorylated tyrosine increase many fold by the activation of tyrosine kinases. Most mitogenic receptor systems such as EGF, PDGF, insulin receptors contain serine/threonine/tyrosine kinase domains that undergo autophosphorylation when receptors bind to the respective ligands. Monoclonal anti-phosphotyrosine?biotin antibody can be used in dot blot (diluted 1:32,000). Mouse anti-phosphotyrosine?biotin antibody reacts specifically with phosphorylated tyrosine both as the free amino acid or when conjugated to BSA or KLH. This product does not react with non-phosphorylated tyrosine or other phosphorylated amino acids, including serine and threonine, or with phosphorylated molecules like AMP or ATP.

Immunogen

phosphotyrosine conjugated to BSA

Application

Monoclonal Anti-Phosphotyrosine?Biotin antibody produced in mouse has been used in western blot analysis to detect tyrosine phosphorylated proteins. It has also been used in BIAcore analysis. This conjugate maybe used as an analytical tool by enabling the identification and quantification of tyrosine phosphorylated proteins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Monoclonal Anti-Phosphotyrosine−Biotin antibody produced in mouse clone PT-66, purified immunoglobulin, buffered aqueous solution

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Monoclonal Anti-Phosphotyrosine–Peroxidase antibody produced in mouse clone PT-66, purified immunoglobulin, lyophilized powder

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Monoclonal Anti-Phosphotyrosine–Peroxidase antibody produced in mouse

description

clone PT-66, purified immunoglobulin, buffered aqueous solution

description

clone PY20, purified immunoglobulin, buffered aqueous glycerol solution

description

clone PT-66, purified from hybridoma cell culture

description

clone PT-66, purified immunoglobulin, lyophilized powder

conjugate

biotin conjugate

conjugate

FITC conjugate

conjugate

unconjugated

conjugate

peroxidase conjugate

clone

PT-66, monoclonal

clone

PY20, monoclonal

clone

PT-66, monoclonal

clone

PT-66, monoclonal

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

technique(s)

dot blot: 1:32,000, direct ELISA: 1:50,000

technique(s)

immunoprecipitation (IP): suitable, enzyme immunoassay: suitable, direct immunofluorescence: 5-10 μg/mL, western blot: suitable

technique(s)

immunoprecipitation (IP): suitable, indirect ELISA: 0.5-1.0 μg/mL using phosphotyrosine conjugated to BSA, immunohistochemistry: suitable, radioimmunoassay: suitable, western blot: 0.25-0.5 μg/mL using total cell extract of human platelets, immunocytochemistry: suitable, flow cytometry: suitable

technique(s)

direct ELISA: 1:60,000 using Phosphotyrosine-BSA, dot blot: 1:40,000-1:200,000 using phosphotyrosine-BSA using chromogenic and chemiluminescent substrates, respectively

isotype

IgG1

isotype

IgG2b

isotype

IgG1

isotype

IgG1

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Storage Class

10 - Combustible liquids

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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