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B3150

Monoclonal Anti-Protein A−Biotin antibody produced in mouse

clone SPA-27, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Monoclonal Anti-Protein A

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0.2 mL
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$568.00
0.5 mL
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$805.00

About This Item

NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
biotin conjugate
Clone:
SPA-27, monoclonal
Application:
ELISA (d)
Citations:
7

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biological source

mouse

Quality Segment

200

conjugate

biotin conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

SPA-27, monoclonal

form

buffered aqueous solution

technique(s)

direct ELISA: 1:80,000-1:160,000

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Protein A is a cell wall associated protein isolated from Staphylococcus aureus Cowan 1 strain. The 42 kDa protein consists of a single polypeptide chain. Protein A possesses a specific affinity for Fc region of many mammalian immunoglobulins as human, rabbit, and guinea pig IgGs and therefore, considered as a universal reagent in biochemistry and immunology. Antibodies to protein A have been produced in rabbits, guinea pigs, chickens and mice. Protein A affinity chromatography has been achieved for the purification of immmunoglobulins. Monoclonal Anti-Protein A (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Immunogen

protein A from Cowan I strain of Staphy­lococcus aureus.

Application

Monoclonal Anti-Protein A-Biotin antibody produced in mouse may be used in ELISA at a working dilution of 1:80,000 - 1:160,000.

Biochem/physiol Actions

Protein A is an immunoglobulin-binding protein present on the surface of Staphylococcus aureus and is also secreted into the extracellular environment. It binds the Fc region of Igs and Fab regions of B cell receptors. The coupling of biotin to monoclonal Anti-Protein A antibody allows for the binding of various labels such as avidin or streptavidin.
Specific for Protein A and shows no cross-reactivity with Protein G. Reacts with free Protein A or IgG bound Protein A and does not interfere with the Fc binding activity of Protein A.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% inactivated bovine serum albumin and 15 mM sodium azide.

Preparation Note

Prepared by conjugation of biotinamidocaproate N-hydroxysuccinimide ester to fractionated monoclonal anti-Protein A.
Store at 2-8 °C for up to one month. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frostfree" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Monoclonal Anti-Protein A−Biotin antibody produced in mouse clone SPA-27, purified immunoglobulin, buffered aqueous solution

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Sigma-Aldrich

SAB4200745

Anti-Protein A antibody, Mouse Monoclonal

description

clone SPA-27, purified immunoglobulin, buffered aqueous solution

description

clone SPA-27, ascites fluid

description

clone PT-66, purified immunoglobulin, buffered aqueous solution

description

clone SPA-27, purified from hybridoma cell culture

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

clone

SPA-27, monoclonal

clone

SPA-27, monoclonal

clone

PT-66, monoclonal

clone

SPA-27, monoclonal

antibody form

purified immunoglobulin

antibody form

ascites fluid

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

conjugate

biotin conjugate

conjugate

unconjugated

conjugate

biotin conjugate

conjugate

-

isotype

IgG1

isotype

IgG1

isotype

IgG1

isotype

IgG1

technique(s)

direct ELISA: 1:80,000-1:160,000

technique(s)

immunocytochemistry: suitable, western blot: suitable, indirect ELISA: 1:20,000

technique(s)

dot blot: 1:32,000, direct ELISA: 1:50,000

technique(s)

immunofluorescence: suitable, dot blot: suitable, immunoblotting: suitable, flow cytometry: suitable, indirect ELISA: 0.25-0.5 μg/mL using 10 μg/mL of protein A from Staphylococcus aureus for coating. To reduce nonspecific binding we recommend on detection using a secondary anti-mouse, F(ab’)2 fragment antibody, immunoprecipitation (IP): 2.5-5 μg/test using purified protein A from Staphylococcus aureus

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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