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Monoclonal Anti-Protein A−Biotin antibody produced in mouse

Name: Monoclonal Anti-Protein A−Biotin antibody produced in mouse
Brand: SIGMA
Price: 568 USD
Availability: InStock

clone SPA-27, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Monoclonal Anti-Protein A

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Size/SKU	Availability	Price
0.2 mL	Check Cart for Availability	$568.00
0.5 mL	Check Cart for Availability	$805.00

About This Item

NACRES:

NA.46

UNSPSC Code:

12352203

Conjugate:

biotin conjugate

Clone:

SPA-27, monoclonal

Application:

ELISA (d)

Citations:

$568.00

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Properties

biological source

mouse

Quality Segment

200

conjugate

biotin conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

SPA-27, monoclonal

form

buffered aqueous solution

technique(s)

direct ELISA: 1:80,000-1:160,000

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

Protein A is a cell wall associated protein isolated from Staphylococcus aureus Cowan 1 strain. The 42 kDa protein consists of a single polypeptide chain. Protein A possesses a specific affinity for Fc region of many mammalian immunoglobulins as human, rabbit, and guinea pig IgGs and therefore, considered as a universal reagent in biochemistry and immunology. Antibodies to protein A have been produced in rabbits, guinea pigs, chickens and mice. Protein A affinity chromatography has been achieved for the purification of immmunoglobulins. Monoclonal Anti-Protein A (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Immunogen

protein A from Cowan I strain of Staphylococcus aureus.

Application

Monoclonal Anti-Protein A-Biotin antibody produced in mouse may be used in ELISA at a working dilution of 1:80,000 - 1:160,000.

Biochem/physiol Actions

Protein A is an immunoglobulin-binding protein present on the surface of Staphylococcus aureus and is also secreted into the extracellular environment. It binds the Fc region of Igs and Fab regions of B cell receptors. The coupling of biotin to monoclonal Anti-Protein A antibody allows for the binding of various labels such as avidin or streptavidin.

Specific for Protein A and shows no cross-reactivity with Protein G. Reacts with free Protein A or IgG bound Protein A and does not interfere with the Fc binding activity of Protein A.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% inactivated bovine serum albumin and 15 mM sodium azide.

Preparation Note

Prepared by conjugation of biotinamidocaproate N-hydroxysuccinimide ester to fractionated monoclonal anti-Protein A.

Store at 2-8 °C for up to one month. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frostfree" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	B6775	B1531	B3398
Sigma-Aldrich B3150 Monoclonal Anti-Protein A−Biotin antibody produced in mouse	Sigma-Aldrich B6775 Anti-Human IgG1−Biotin antibody, Mouse monoclonal	Sigma-Aldrich B1531 Monoclonal Anti-Phosphotyrosine−Biotin antibody produced in mouse	Sigma-Aldrich B3398 Anti-Human IgG2−Biotin antibody, Mouse monoclonal
description clone SPA-27, purified immunoglobulin, buffered aqueous solution	description clone 8c/6-39, purified from hybridoma cell culture	description clone PT-66, purified immunoglobulin, buffered aqueous solution	description clone HP-6014, purified from hybridoma cell culture
clone SPA-27, monoclonal	clone 8c/6-39, monoclonal	clone PT-66, monoclonal	clone HP-6014, monoclonal
conjugate biotin conjugate	conjugate biotin conjugate	conjugate biotin conjugate	conjugate biotin conjugate
biological source mouse	biological source mouse	biological source mouse	biological source mouse
antibody form purified immunoglobulin	antibody form purified from hybridoma cell culture	antibody form purified immunoglobulin	antibody form purified from hybridoma cell culture
technique(s) direct ELISA: 1:80,000-1:160,000	technique(s) direct ELISA: 1:1,000	technique(s) dot blot: 1:32,000, direct ELISA: 1:50,000	technique(s) direct ELISA: 1:30,000
form buffered aqueous solution	form buffered aqueous solution	form buffered aqueous solution	form buffered aqueous solution

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Storage Class

10 - Combustible liquids

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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