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B7405

Anti-Digoxin−Biotin antibody, Mouse monoclonal

clone DI-22, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-Digoxin, Monoclonal Anti-Digoxin−Biotin antibody produced in mouse

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0.2 mL

Available to ship TODAYfromMILWAUKEE

$311.00
0.5 mL

Available to ship TODAYfromMILWAUKEE

$535.00

About This Item

NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
biotin conjugate
Clone:
DI-22, monoclonal
Application:
ELISA (i), WB
Citations:
10

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biological source

mouse

Quality Segment

200

conjugate

biotin conjugate

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

DI-22, monoclonal

form

buffered aqueous solution

technique(s)

indirect ELISA: 1:10,000 using digoxin-labeled biomolecule, western blot: 1:500 using using digoxin-labeled biomolecule

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Monoclonal Anti-Digoxin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Immunogen

Digoxin-KLH.

Application

Monoclonal Anti-Digoxin-Biotin antibody produced in mouse has been used in:
  • in situ hybridization
  • immunohistochemistry
  • immunolocalization

Monoclonal Anti-Digoxin-Biotin antibody produced in mouse may be used for ELISA at a dilution of 1:10,000 and for dot blots at a dilution of 1:500. It was also used to stain apoptotic cells in cervical carcinoma sections by TUNEL technique.

Biochem/physiol Actions

Antibody is specific for digoxin and shows a high affinity for digoxigenin.
Digoxin is a phytoestrogen that binds estrogen receptors and produces inotropic effects in cardiac muscle. Studies show that digoxin decreases the risk of breast and uterus cancers that are estrogen sensitive.
The conjugate may be used to detect digoxin-labeled compounds such as oligonucleotides, antibodies or peptides.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Anti-Digoxin−Biotin antibody, Mouse monoclonal clone DI-22, purified from hybridoma cell culture

Sigma-Aldrich

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Anti-Digoxin−Biotin antibody, Mouse monoclonal

Monoclonal Anti-Digoxin–FITC antibody produced in mouse clone DI-22, purified immunoglobulin, buffered aqueous solution

Sigma-Aldrich

F3523

Monoclonal Anti-Digoxin–FITC antibody produced in mouse

Monoclonal Anti-Digoxin antibody produced in mouse clone DI-22, ascites fluid

Sigma-Aldrich

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Monoclonal Anti-Digoxin antibody produced in mouse

Anti-Digoxin–Alkaline Phosphatase antibody, Mouse monoclonal clone DI-22, purified from hybridoma cell culture

Sigma-Aldrich

A1054

Anti-Digoxin–Alkaline Phosphatase antibody, Mouse monoclonal

description

clone DI-22, purified from hybridoma cell culture

description

clone DI-22, purified immunoglobulin, buffered aqueous solution

description

clone DI-22, ascites fluid

description

clone DI-22, purified from hybridoma cell culture

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

conjugate

biotin conjugate

conjugate

FITC conjugate

conjugate

unconjugated

conjugate

alkaline phosphatase conjugate

clone

DI-22, monoclonal

clone

DI-22, monoclonal

clone

DI-22, monoclonal

clone

DI-22, monoclonal

antibody form

purified from hybridoma cell culture

antibody form

purified immunoglobulin

antibody form

ascites fluid

antibody form

purified from hybridoma cell culture

technique(s)

indirect ELISA: 1:10,000 using digoxin-labeled biomolecule, western blot: 1:500 using using digoxin-labeled biomolecule

technique(s)

dot immunobinding: 1:16 using digoxigenin-labeled biomolecule

technique(s)

dot blot: suitable, flow cytometry: suitable, indirect ELISA: 1:10,000 using digoxin-BSA, indirect ELISA: 1:2,500 using digoxigenin-transferrin

technique(s)

direct ELISA: 1:10,000 using using 20 μg/ml of purified digoxin-BSA for coating and pNPP substrate, direct ELISA: 1:6,000 using using 10 μg/ml of digoxigenin-transferrin for coating and pNPP substrate, dot blot: 1:20,000 using digoxigenin-labeled biomolecules (chemiluminescent)

form

buffered aqueous solution

form

buffered aqueous solution

form

-

form

buffered aqueous solution

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Storage Class

10 - Combustible liquids

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nwg

flash_point_f

Not applicable

flash_point_c

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