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Anti-Digoxin−Biotin antibody, Mouse monoclonal

Name: Anti-Digoxin−Biotin antibody, Mouse monoclonal
Brand: SIGMA
Price: 311 USD

clone DI-22, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-Digoxin, Monoclonal Anti-Digoxin−Biotin antibody produced in mouse

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Pack Size	SKU	Availability	Price
0.2 mL		Check Cart for Availability	$311.00
0.5 mL		Check Cart for Availability	$535.00

About This Item

NACRES:

NA.46

UNSPSC Code:

12352203

Conjugate:

biotin conjugate

Clone:

DI-22, monoclonal

Application:

ELISA (i), WB

Citations:

$311.00

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Properties

biological source

mouse

Quality Level

200

conjugate

biotin conjugate

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

DI-22, monoclonal

form

buffered aqueous solution

technique(s)

indirect ELISA: 1:10,000 using digoxin-labeled biomolecule, western blot: 1:500 using using digoxin-labeled biomolecule

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

Monoclonal Anti-Digoxin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Immunogen

Digoxin-KLH.

Application

Monoclonal Anti-Digoxin-Biotin antibody produced in mouse has been used in:

in situ hybridization
immunohistochemistry
immunolocalization

Monoclonal Anti-Digoxin-Biotin antibody produced in mouse may be used for ELISA at a dilution of 1:10,000 and for dot blots at a dilution of 1:500. It was also used to stain apoptotic cells in cervical carcinoma sections by TUNEL technique.

Biochem/physiol Actions

Antibody is specific for digoxin and shows a high affinity for digoxigenin.

Digoxin is a phytoestrogen that binds estrogen receptors and produces inotropic effects in cardiac muscle. Studies show that digoxin decreases the risk of breast and uterus cancers that are estrogen sensitive.

The conjugate may be used to detect digoxin-labeled compounds such as oligonucleotides, antibodies or peptides.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	SAB4200669	D8156	A1054
Sigma-Aldrich B7405 Anti-Digoxin−Biotin antibody, Mouse monoclonal	Sigma-Aldrich SAB4200669 Anti-Digoxin antibody, Mouse monoclonal	Sigma-Aldrich D8156 Monoclonal Anti-Digoxin antibody produced in mouse	Sigma-Aldrich A1054 Anti-Digoxin–Alkaline Phosphatase antibody, Mouse monoclonal
conjugate biotin conjugate	conjugate -	conjugate unconjugated	conjugate alkaline phosphatase conjugate
antibody form purified from hybridoma cell culture	antibody form purified from hybridoma cell culture	antibody form ascites fluid	antibody form purified from hybridoma cell culture
clone DI-22, monoclonal	clone DI-22, monoclonal	clone DI-22, monoclonal	clone DI-22, monoclonal
biological source mouse	biological source mouse	biological source mouse	biological source mouse
technique(s) indirect ELISA: 1:10,000 using digoxin-labeled biomolecule, western blot: 1:500 using using digoxin-labeled biomolecule	technique(s) ELISA: 0.15-0.3 μg/mL	technique(s) dot blot: suitable, flow cytometry: suitable, indirect ELISA: 1:10,000 using digoxin-BSA, indirect ELISA: 1:2,500 using digoxigenin-transferrin	technique(s) direct ELISA: 1:10,000 using using 20 μg/ml of purified digoxin-BSA for coating and pNPP substrate, direct ELISA: 1:6,000 using using 10 μg/ml of digoxigenin-transferrin for coating and pNPP substrate, dot blot: 1:20,000 using digoxigenin-labeled biomolecules (chemiluminescent)
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Global Trade Item Number

SKU	GTIN
B7405-.5ML	04061837856372
B7405-.2ML	04061837856365

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