The specific site of biotin attachment on the IgG molecule has not been determined.
Additionally, the biotinylation procedure is proprietary and, therefore, cannot be disclosed.
B9183
Anti-HA-Biotin antibody, Mouse monoclonal
clone HA-7, purified from hybridoma cell culture
Synonym(s):
Monoclonal Anti-HA, Monoclonal Anti-HA-Biotin antibody produced in mouse, Anti-HA, Anti-Influenza Hemagglutinin
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 100 μg | Available to ship TODAYfromMILWAUKEE | $511.00 |
About This Item
Conjugate:
biotin conjugate
Clone:
HA-7, monoclonal
Application:
WB CL
Citations:
7
$511.00
Available to ship TODAYDetails
biological source
mouse
Quality Level
conjugate
biotin conjugate
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
HA-7, monoclonal
form
buffered aqueous solution
concentration
~1 mg/mL
technique(s)
western blot (chemiluminescent): 0.25-0.50 μg/mL using HA tagged fusion proteins in transiently transfected mammalian cell extracts.
shipped in
dry ice
storage temp.
−20°C, 2-8°C
General description
The Monoclonal Anti-HA Biotin Conjugate antibody recognizes the HA tag sequence on the HA tagged fusion proteins when expressed at the N or C terminal to the fusion protein. This antibody is derived from the HA-7 hybridoma, which is produced from the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the amino acid residues 98-106 (YPYDVPDYA) of human Influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH. Ascites fluid collected from the HA-7 hybridoma is then conjugated to biotin.
Immunogen
synthetic peptide corresponding to a fragment of human influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH
Application
Antibody suitable for immunoblotting.
Biochem/physiol Actions
The antibody recognizes native as well as denatured-reduced forms of HA-tagged proteins and is reactive with N- or C-terminal HA-tagged fusion proteins expressed in E. coli or in mammalian cells.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
1 of 1
This Item | |||
|---|---|---|---|
| clone HA-7, monoclonal | clone HA-7, monoclonal | clone HA-7, monoclonal | clone HA-7, monoclonal |
| antibody form purified from hybridoma cell culture | antibody form purified from hybridoma cell culture | antibody form purified immunoglobulin | antibody form purified from hybridoma cell culture |
| conjugate biotin conjugate | conjugate TRITC conjugate | conjugate unconjugated | conjugate alkaline phosphatase conjugate |
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in wet ice |
| technique(s) western blot (chemiluminescent): 0.25-0.50 μg/mL using HA tagged fusion proteins in transiently transfected mammalian cell extracts. | technique(s) direct immunofluorescence: 10-15 mg/mL using mammalian cells expressing HA-tagged fusion proteins | technique(s) immunocytochemistry: 1.0-2.0 μg/mL, immunoprecipitation (IP): 0.5-2.0 μg, western blot: 0.25-1 μg/mL | technique(s) western blot: 1:4,000 using extracts of mammalian cells expressing HA tagged fusion proteins |
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Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| B9183-100UG | 04061838069337 |
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Where is the biotin located on the IgG? Additionally, what protocol or method did you follow to biotinylate it?
1 answer-
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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What is the isotype and light chain for Monoclonal Anti-HA antibody clone HA-7 produced in mouse?
1 answer-
Monoclonal Anti-HA antibody clone HA-7 is mouse IgG1, kappa light chain.
Helpful?
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