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Monoclonal Anti-Bin1 antibody produced in mouse

Name: Monoclonal Anti-Bin1 antibody produced in mouse
Brand: SIGMA
Price: 744 USD
Availability: InStock

clone 99D, ascites fluid

Synonym(s):

Anti-Amphiphysin II, Anti-SH3P9, Anti-ampl

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Size/SKU	Availability	Price
0.2 mL	Estimated to ship TODAYfromMILWAUKEE	$744.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD02095896

Conjugate:

unconjugated

Clone:

99D, monoclonal

Application:

ELISA (i), FACS, ICC, IHC (f), IP, WB

Citations:

$744.00

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

99D, monoclonal

mol wt

antigen 65-75 kDa

contains

15 mM sodium azide

species reactivity

rabbit, mouse, rat, avian, human

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable using nucleoplasm, subnuclear punctate compartment, and cytoplasm, immunohistochemistry (frozen sections): suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, western blot: 1:50,000 using a rat brain cytosol preparation

isotype

IgG2b

UniProt accession no.

O00499

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... BIN1(274)
mouse ... Bin1(30948)
rat ... Bin1(117028)

Description

General description

Monoclonal Anti-Bin1 (SH3P9, Amphiphysin II, amphl) (mouse IgG2b isotype) is derived from the 99D hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a recombinant polypeptide, containing amino acids 189-398 of human Bin1. Bin1 [Box-dependent myc-interacting protein-1, also known as SH3P9, amphiphysin II and amphl (amphiphysin-like)] is a novel protein that has features of a tumor suppressor. The human BIN1 gene has been mapped to chromosome 2q14. In human and rodent cells it appears as a short-lived (half-life of approx. 2 hrs) monomeric phosphoprotein (approx. 70 kDa). Bin1 molecule appears in different isoforms or conformations, and the localization varies between normal and tumor cells. In normal cells, Bin1 is predominantly nucleoplasmic, but is also present in a subnuclear compartment. In a panel of tumor cells that express Bin1, the predominant localization of the molecule is in the subnuclear compartment.

Immunogen

recombinant polypeptide containing amino acids 189-398 of human Bin1.

Application

Monoclonal Anti-Bin1 antibody produced in mouse has been used in:

western blotting
enzyme linked immunosorbent assay (ELISA)
immunoprecipitation
immunocytochemistry
immunohistochemistry
flow cytometry

Biochem/physiol Actions

Box-dependent myc-interacting protein-1 (Bin1) interacts with and inhibits the oncogenic activity of the myc oncoprotein that has a major role in many human cancers. The loss of Bin1 may contribute to growth deregulation in cancer cells in carcinoma of the breast, colon, lung, cervix, prostate and liver. In bin1 locus mid-2q region that is deleted in approx. 42% of metastatic prostate cancers, and at the syntenic murine locus, in >90% of radiation-induced myeloid leukemias. In addition to the role of Bin1 in controlling neoplastic cell growth and cell cycle by the inhibition of transformation by both myc and the adenovirus E1A protein. Deficits in expression are functionally significant, because ectopic Bin1 can inhibit the growth of tumor cells that lack endogenous expression. Bin1 has also been found to be highly expressed in murine skeletal muscle; overexpression of Bin1 promotes myotube formation and upregulation of myosin heavy chain, while interference with Bin1 expression significantly impairs these processes.

The antibody reacts specifically with a 33-residue segment of the myc-binding domain (MBD, amino acids 323-356) of the human Bin1 molecule. It does not react with the Bin1-related 45 kDa polypeptide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	C7464	M4308	M4278
Sigma-Aldrich B9428 Monoclonal Anti-Bin1 antibody produced in mouse	Sigma-Aldrich C7464 Monoclonal Anti-Cyclin D1 antibody produced in mouse	Sigma-Aldrich M4308 Monoclonal Anti-MDM2 antibody produced in mouse	Sigma-Aldrich M4278 Monoclonal Anti-MAP1 antibody produced in mouse
clone 99D, monoclonal	clone DCS-6, monoclonal	clone SMP14, monoclonal	clone HM-1, monoclonal
biological source mouse	biological source mouse	biological source mouse	biological source mouse
antibody form ascites fluid	antibody form ascites fluid	antibody form ascites fluid	antibody form ascites fluid
Gene Information human ... BIN1(274) mouse ... Bin1(30948) rat ... Bin1(117028)	Gene Information human ... CCND1(595) mouse ... Ccnd1(12443) rat ... Ccnd1(58919)	Gene Information human ... MDM2(4193) mouse ... Mdm2(17246) rat ... Mdm2(246362)	Gene Information mouse ... Mtap1a(17754) rat ... Map1a(25152)
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
species reactivity rabbit, mouse, rat, avian, human	species reactivity rat, mouse, human, monkey	species reactivity human, rat, mouse (weak)	species reactivity mouse, rat

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c