B9673
1-Bromo-3-chloropropane
for isolation of RNA
Sinônimo(s):
1-Bromo-3-chloropropane, I-BCP, Trimethylene bromochloride, Trimethylene chlorobromide
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Sobre este item
Fórmula linear:
Cl(CH2)3Br
Número CAS:
Peso molecular:
157.44
UNSPSC Code:
41105515
NACRES:
NA.52
PubChem Substance ID:
EC Number:
203-697-1
Beilstein/REAXYS Number:
605278
MDL number:
Assay:
≥98.5% (GC)
Grade:
Molecular Biology
Bp:
144-145 °C (lit.)
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Molecular Biology
Quality Segment
assay
≥98.5% (GC)
form
liquid
refractive index
n20/D 1.486 (lit.)
bp
144-145 °C (lit.)
density
1.592 g/mL at 25 °C (lit.)
storage temp.
room temp
SMILES string
ClCCCBr
InChI
1S/C3H6BrCl/c4-2-1-3-5/h1-3H2
InChI key
MFESCIUQSIBMSM-UHFFFAOYSA-N
General description
BCP can be used in place of chloroform and is less toxic. The use of BCP in the single-step TRI reagent method decreases the possibility of contaminating RNA with DNA. It does not adversely affect quality or quantity of the isolated RNA. 1-Bromo-3-chloropropane is a phase separation reagent used in protocols for isolation of nucleic acids.
Application
1-Bromo-3-chloropropane has been used in molecular biology applications for the isolation of high quality RNA from:
- feline teeth
- mice brain and liver tissues
- human ectocervical epithelial cells
- placental samples
- human mesenchymal stromal cells (hMSCs)[1]
Features and Benefits
- less toxic alternative to chloroform
- compatible with TRI reagent for isolation of RNA with decreased DNA contamination
Other Notes
BCP can be used in place of chloroform, and is less toxic. The use of BCP in the single-step TRI Reagent method decreases the possibility of contaminating RNA with DNA. It does not adversely affect quality or quantity of the isolated RNA.
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signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Muta. 2 - STOT SE 3
target_organs
Respiratory system
Classe de armazenamento
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 2
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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