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Monoclonal Anti-Calcineurin (β-Subunit) antibody produced in mouse

Name: Monoclonal Anti-Calcineurin (β-Subunit) antibody produced in mouse
Brand: SIGMA
Price: 482.8 USD
Availability: InStock

clone CN-B1, ascites fluid

Synonym(s):

Anti-CALNB1, Anti-CNB, Anti-CNB1

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Size/SKU	Availability	Price
0.2 mL	Available to ship TODAYfromMILWAUKEE	$568.00 $482.80

About This Item

UNSPSC Code:

12352203

NACRES:

NA.44

MDL number:

MFCD00282169

Conjugate:

unconjugated

Clone:

CN-B1, monoclonal

Application:

ELISA (i), IHC (p), WB

Citations:

$482.80

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

CN-B1, monoclonal

contains

15 mM sodium azide

species reactivity

bovine, rat, human, mouse

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using neurons in human myenteric and prostatic ganglia, indirect ELISA: suitable, western blot: 1:3,000 using rat brain extract

isotype

IgG2b

UniProt accession no.

P63098

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PPP3R1(5534), PPP3R2(5535)
rat ... Ppp3r1(29748), Ppp3r2(29749)

Description

General description

Calcineurin (CaN, heterodimeric enzyme) is a Ca(²⁺)/calmodulin (CaM)-stimulated protein phosphatase 3 (PPP3) consisting of a catalytic A subunit (CnA) and a Ca(²⁺)-binding regulatory B subunit (CnB). The calcium-binding regulatory subunit (calcineurin B) is coded by the PPP3R1 gene located on human chromosome 2. Protein phosphatase 3, regulatory subunit B, α (PPP3R1) is also known as CALN, CCN1, CNA1, CALNA, PPP2B and CALNA1. The regulatory subunit (calcineurin B) is widely expressed in brain while the catalytic subunit (calcineurin A) is abundantly expressed in Hassall′s corpuscles, localized in the thymic medulla and represents the terminal stages of thymic medullary epithelium.

Monoclonal Anti-Calcineurin (β-subunit) (mouse IgM isotype) is derived from the CN-B1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified bovine brain calcineurin preparation. Two different calcineurin B has been reported in rat testis.

Immunogen

bovine brain calcineurin

Application

Monoclonal Anti-Calcineurin (b-Subunit) antibody produced in mouse has been used in immunohistochemistry, indirect ELISA and western blotting.

Biochem/physiol Actions

Calcineurin calcium -binding subunit, calcineurin B, is immunologically conserved from yeast to mammals. immunoreactivity is detected at significant concentrations only in normal and neoplastic neurons regions of the cytoplasm dendrites, axons and axon terminals. A close correlation has been observed between inhibition of calcineurin by the complexes of cyclosporin A/cyclophilin and tacrolimus(FK506) and FK506 binding proteins (FKBPs) and inhibition of apoptosis. Calcineurin is an excellent marker enzyme for the detection of neuronal activity and synaptic plasticity after brain damage, such as an ischemic insult.

Calcineurin is a major soluble calmodulin-binding protein in the brain. It stimulates the expression of the surface molecules CD83, CD80, CD86, CD40 and HLA-DR by promoting secretion of inflammatory cytokines IL-6, TNF-α and IL-1β by human PBMC-derived dendritic cells. It plays an important role in signal transduction, activation of T cell. Calcineurin is an excellent marker enzyme for the detection of neuronal activity and synaptic plasticity after brain damage.

In immunoblotting, the antibody recognizes an epitope located on the β-subunit of calcineurin (18 kDa, also called calcineurin B) and does not cross-react with the α-subunit.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	D219	A276	WH0005530M3
Sigma-Aldrich C0581 Monoclonal Anti-Calcineurin (β-Subunit) antibody produced in mouse	Sigma-Aldrich D219 Monoclonal Anti-Dihydropyridine Receptor (α₂ Subunit) antibody produced in mouse	Sigma-Aldrich A276 Monoclonal Anti-Na⁺/K⁺ ATPase (α Subunit) antibody produced in mouse	Sigma-Aldrich WH0005530M3 Monoclonal Anti-PPP3CA antibody produced in mouse
description clone CN-B1, ascites fluid	description clone 20A, ascites fluid, buffered aqueous solution	description clone M7-PB-E9, ascites fluid	description clone 2G8, purified immunoglobulin, buffered aqueous solution
biological source mouse	biological source mouse	biological source mouse	biological source mouse
species reactivity bovine, rat, human, mouse	species reactivity rat, mouse, rabbit, guinea pig	species reactivity canine, chicken, human, sheep, pig, bovine, mouse	species reactivity human
Gene Information human ... PPP3R1(5534), PPP3R2(5535) rat ... Ppp3r1(29748), Ppp3r2(29749)	Gene Information mouse ... Cacna2d1(12293) rat ... Cacna2d1(25399)	Gene Information human ... ATP1A1(476)	Gene Information human ... PPP3CA(5530)
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
antibody form ascites fluid	antibody form ascites fluid	antibody form ascites fluid	antibody form purified immunoglobulin
clone CN-B1, monoclonal	clone 20A, monoclonal	clone M7-PB-E9, monoclonal	clone 2G8, monoclonal

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Storage Class

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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