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Anti-CD137 antibody produced in goat

Name: Anti-CD137 antibody produced in goat
Brand: SIGMA
Price: 533.8 USD
Availability: InStock

affinity isolated antibody, lyophilized powder

Synonym(s):

Anti-4-1BB

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Size/SKU	Availability	Price
0.1 mg	Check Cart for Availability	$628.00 $533.80

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD01094424

Conjugate:

unconjugated

Clone:

polyclonal

Application:

ELISA (c), FACS, ICC, WB

Citations:

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Properties

biological source

goat

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized powder

species reactivity

human

technique(s)

capture ELISA: 0.2-0.8 μg/mL, flow cytometry: 2.5 μg using 10⁶ human T cells treated with PHA, immunocytochemistry: 5-15 μg/mL, western blot: 0.1 μg/mL

impurities

<0.10 EU/μg endotoxin

UniProt accession no.

Q07011

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TNFRSF9(3604)

Description

General description

CD137 (4-1BB) is a member of the tumor necrosis factor (TNF) receptor superfamily. It is a type I transmembrane glycoprotein expressed on the cell surface of activated splenic T cells and thymocytes.

Immunogen

mouse myeloma cell line NS0-derived recombinant human 41BB/TNFRSF9/CD137 Leu²⁴Gln¹⁸⁶

Application

Anti-CD137 antibody produced in goat is suitable for the following applications:

Agonist Activity: Measured by its ability to costimulate IFNg secretion by human T cells in the presence of anti-CD3. The ED50 for this effect is typically 5-20μg/mL.
ELISA capture: It can be used as a capturing reagent in a human 4-1BB sandwich immunoassay in combination with biotinylated human 4-1BB detection antibody and recombinant human 4-1BB as the standard. The suggested coating concentration range is 0.2-0.8μg/ml. The recommended concentration of the detection antibody is 0.1-0.4μg/mL.
Flow cytometry: 2.5μg/106 cells using Human T cells treated with PHA (phytohemagglutinin).
Immunoblotting: A working concentration of 0.1μg/ml was determined using recombinant human CD137.
Immunocytochemistry: 5-15μg/mL using immersion fixed human peripheral blood mononuclear cells.

Biochem/physiol Actions

CD137 in T lymphocytes helps to regulate activation, proliferation and apoptosis. CD137 and CD28 are co-stimulatory molecules of T cell activation. It plays an important role in regulating T-cell-dependent immune responses. The expression of CD137 correlates negatively with lymphocyte proliferation and induces cell death caused by mitogen overstimulation. In monocytes, CD137 induces activation, promotes adherence and helps in prolongs survival.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.

Preparation Note

Reconstitute at 0.2 mg/mL in sterile PBS

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	SAB4701068	I8409	T2315
Sigma-Aldrich C0730 Anti-CD137 antibody produced in goat	Sigma-Aldrich SAB4701068 Monoclonal Anti-CD137-APC antibody produced in mouse	Sigma-Aldrich I8409 Anti-Interleukin-7 Receptor α/CD127 antibody produced in goat	$Anti-Tumor Necrosis Factor Soluble Receptor Type II antibody produced in goat IgG fraction of antiserum, lyophilized powder$ Sigma-Aldrich T2315 Anti-Tumor Necrosis Factor Soluble Receptor Type II antibody produced in goat
description affinity isolated antibody, lyophilized powder	description clone 4B4-1	description affinity isolated antibody, lyophilized powder	description IgG fraction of antiserum, lyophilized powder
conjugate unconjugated	conjugate Allophycocyanin conjugate	conjugate unconjugated	conjugate unconjugated
antibody form affinity isolated antibody	antibody form purified immunoglobulin	antibody form affinity isolated antibody	antibody form IgG fraction of antiserum
biological source goat	biological source mouse	biological source goat	biological source goat
technique(s) capture ELISA: 0.2-0.8 μg/mL, immunocytochemistry: 5-15 μg/mL, flow cytometry: 2.5 μg using 10⁶ human T cells treated with PHA, western blot: 0.1 μg/mL	technique(s) flow cytometry: 10 μL	technique(s) western blot: 0.1-0.2 μg/mL, flow cytometry: 5.0-10.0 μg/mL	technique(s) immunocytochemistry: 5-15 μg/mL (detects TNF RII in human PBLs), western blot: 1 μg/mL (detects rhs TNF RII at 5 ng/lane under non-reducing conditions)
species reactivity human	species reactivity -	species reactivity human	species reactivity human
form lyophilized powder	form buffered aqueous solution	form lyophilized powder	form lyophilized powder

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Storage Class

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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