C0730
Anti-CD137 antibody produced in goat
affinity isolated antibody, lyophilized powder
Synonym(s):
Anti-4-1BB
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 0.1 mg | Available to ship TODAYfromMILWAUKEE | $628.00 $533.80 |
About This Item
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ELISA (c), FACS, ICC, WB
Citations:
6
$533.80
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Available to ship TODAYDetails
biological source
goat
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized powder
species reactivity
human
technique(s)
capture ELISA: 0.2-0.8 μg/mL, flow cytometry: 2.5 μg using 106 human T cells treated with PHA, immunocytochemistry: 5-15 μg/mL, western blot: 0.1 μg/mL
impurities
<0.10 EU/μg endotoxin
UniProt accession no.
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... TNFRSF9(3604)
General description
CD137 (4-1BB) is a member of the tumor necrosis factor (TNF) receptor superfamily. It is a type I transmembrane glycoprotein expressed on the cell surface of activated splenic T cells and thymocytes.
Immunogen
mouse myeloma cell line NS0-derived recombinant human 41BB/TNFRSF9/CD137 Leu24Gln186
Application
Anti-CD137 antibody produced in goat is suitable for the following applications:
- Agonist Activity: Measured by its ability to costimulate IFNg secretion by human T cells in the presence of anti-CD3. The ED50 for this effect is typically 5-20μg/mL.
- ELISA capture: It can be used as a capturing reagent in a human 4-1BB sandwich immunoassay in combination with biotinylated human 4-1BB detection antibody and recombinant human 4-1BB as the standard. The suggested coating concentration range is 0.2-0.8μg/ml. The recommended concentration of the detection antibody is 0.1-0.4μg/mL.
- Flow cytometry: 2.5μg/106 cells using Human T cells treated with PHA (phytohemagglutinin).
- Immunoblotting: A working concentration of 0.1μg/ml was determined using recombinant human CD137.
- Immunocytochemistry: 5-15μg/mL using immersion fixed human peripheral blood mononuclear cells.
Biochem/physiol Actions
CD137 in T lymphocytes helps to regulate activation, proliferation and apoptosis. CD137 and CD28 are co-stimulatory molecules of T cell activation. It plays an important role in regulating T-cell-dependent immune responses. The expression of CD137 correlates negatively with lymphocyte proliferation and induces cell death caused by mitogen overstimulation. In monocytes, CD137 induces activation, promotes adherence and helps in prolongs survival.
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.
Preparation Note
Reconstitute at 0.2 mg/mL in sterile PBS
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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This Item | |||
|---|---|---|---|
| antibody form affinity isolated antibody | antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form affinity isolated antibody |
| conjugate unconjugated | conjugate unconjugated | conjugate Allophycocyanin conjugate | conjugate unconjugated |
| biological source goat | biological source mouse | biological source mouse | biological source goat |
| Gene Information human ... TNFRSF9(3604) | Gene Information human ... TNFRSF9(3604) | Gene Information human ... TNFRSF9(3604) | Gene Information human ... IL7R(3575) |
| UniProt accession no. | UniProt accession no. - | UniProt accession no. - | UniProt accession no. |
| form lyophilized powder | form buffered aqueous solution | form buffered aqueous solution | form lyophilized powder |
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Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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G Kienzle et al.
International immunology, 12(1), 73-82 (1999-12-23)
Human CD137 is a member of the tumor necrosis factor (TNF) receptor family and the homologue of murine 4-1BB. Recent studies have demonstrated that CD137 promotes accessory T cell activation, and regulates proliferation and survival of T lymphocytes. This study
J Langstein et al.
Journal of leukocyte biology, 65(6), 829-833 (1999-06-25)
CD137 (ILA/4-1BB), a member of the tumor necrosis factor (TNF) receptor family, promotes adherence and prolongs survival of human peripheral monocytes. It induces a strong expression of macrophage colony-stimulating factor (M-CSF), an essential monocyte survival factor. Monocyte survival induced by
J C Hurtado et al.
Journal of immunology (Baltimore, Md. : 1950), 155(7), 3360-3367 (1995-10-01)
The expression of the murine T cell Ag 4-1BB, a member of the TNF-R family, is induced by T cell activation. Previously, we and others had shown that signaling through 4-1BB enhanced proliferative T cell responses. To investigate a potential
Global Trade Item Number
| SKU | GTIN |
|---|---|
| C0730-.1MG | 04061833420072 |