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Normal Human Epidermal Melanocytes 3 (NHEM 3)

Name: Normal Human Epidermal Melanocytes 3 (NHEM 3)
Brand: SIGMA

Adult, isolated in PMA-free Melanocyte Growth Medium M3, 500,000 cryopreserved cells

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About This Item

NACRES:

NA.81

UNSPSC Code:

41106514

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Properties

packaging

pkg of 500,000 cells

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Description

General description

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Application

Primary Normal Human Epidermal Melanocytes (NHEM) are isolated from the epidermis of adult skin (different locations) and are provided in a cryopreserved format. Melanocytes are located in the stratum basale, but branch out between the keratinocytes in suprabasal layers. About 5-10 % of the cells in the epidermis are melanocytes. The main purpose of melanocytes is the production of melanin, the protein responsible for the pigmentation of the skin, eyes, and hair. Melanin protects the cells in the skin and in deeper layers from the hazardous effects of UV radiation. It is produced and stored in melanosomes, which are located close to the melanocyte membrane, but it can also be transferred to neighboring keratinocytes.

Normal Human Epidermal Melanocytes M3 are isolated using the serum-free, BPE-free and PMA-free Melanocyte Growth Medium M3. Since PMA is a tumor promoting mitogen, it can interfere with experimental approaches. Therefore, we recommend using cells isolated in Melanocyte Growth Medium M3 and also using this medium for cultivation. Normal Human Epidermal Keratinocytes (NHEK) and Normal Human Dermal Fibroblasts (NHDF) from the same donor are available on request.

Preparation Note

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Melanocyte Growth Medium M3 (C-24310)

Analysis Note

Rigid quality control tests are performed for each lot of Normal Human Epidermal Melanocytes. They are tested for cell morphology, adherence rate and cell viability. Furthermore, immunohistochemical tests for the cell-type specific marker, Mel-5, are carried out for each lot. Growth performance is tested through multiple passages up to 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Other Notes

Recommended Plating Density: 5000 - 10000 cells per cm²
Passage After Thawing: P2
Tested Markers: Mel-5 positive
Guaranteed population doublings: > 15

Disclaimer

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

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This Item	C-12422	C-14045	C-14044
Sigma-Aldrich C-12413 Normal Human Epidermal Melanocytes 3 (NHEM 3)	Sigma-Aldrich C-12422 Normal Human Epidermal Melanocytes 3 (NHEM 3)	Sigma-Aldrich C-14045 Normal Human Epidermal Melanocytes 3 (NHEM 3)	Sigma-Aldrich C-14044 Normal Human Epidermal Melanocytes 3 (NHEM 3)
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
packaging pkg of 500,000 cells	packaging pkg of 500,000 cells	packaging -	packaging -
storage temp. −196°C	storage temp. −196°C	storage temp. −20°C	storage temp. −20°C
shipped in dry ice	shipped in dry ice	shipped in dry ice	shipped in dry ice

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Substances Subject to be Indicated Names

ishl_indicated

Substances Subject to be Notified Names

ishl_notified

C-12413:

jan

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