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C270

Monoclonal Anti-Calpastatin antibody produced in mouse

Name: Monoclonal Anti-Calpastatin antibody produced in mouse
Brand: SIGMA

clone 1F7E3D10, ascites fluid, buffered aqueous solution

Sinônimo(s):

Anti-BS-17, Anti-PLACK

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Sobre este item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD00164555

Conjugate:

unconjugated

Clone:

1F7E3D10, monoclonal

Application:

IF, WB

Citations:

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Propriedades

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

1F7E3D10, monoclonal

form

buffered aqueous solution

species reactivity

human, rat, bovine, pig

technique(s)

indirect immunofluorescence: 1:200, western blot: 1:5,000

isotype

IgG1

UniProt accession no.

P20810

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CAST(831)
rat ... Cast(25403)

Descrição

General description

Calpastatin is a protein encoded by the CAST gene in humans. Calpastatin is an endogenous inhibitor of the ubiquitous calcium dependent proteinases μ- and m-calpain. It plays a key role in many cellular physiological and pathological processes. Calpastatin level is very important for controlling calpain activity.

Immunogen

calpastatin from bovine skeletal muscle.

Application

Monoclonal Anti-Calpastatin antibody produced in mouse is suitable for indirect immunofluorescence at a dilution of 1:200 and for western blotting at a dilution of 1:5,000.

Biochem/physiol Actions

Calpastatin availability in cytosol is controlled by Ca²⁺ and cyclic AMP. Prolonged calpain activation promotes degradation of calpastatin. Calpastatin is found to be overexpressed in mycoplasma-contaminated cultured cells. CAST gene is found to be involved in genetic susceptibility to Keratoconus (KC), a genetically heterogeneous corneal dystrophy. It is also found to be associated with Parkinson′s disease (PD) and single nucleotide polymorphisms (SNPs).

Epitope mapping studies indicate the epitope is between amino acids 543-673 (domain IV) of human calpastatin. By immunoblotting, the antibody may detect bands at 150, 125, 90 and 70 kDa, which have been suggested to be proteins translated from different start sites of the calpastatin gene. It does not cross-react with calpains or calmodulin. By immunoblotting, reactivity is observed with human platelets and erythrocytes, bovine platelets, heart and skeletal muscle and in rat myoblasts, kidney, liver and spleen. By immunofluorescence on pig LLC-PK1 cells, diffuse cytoplasmic staining is observed.

Physical form

Solution in phosphate buffered saline and contains 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Este Item	C268	C3545	P3088
Sigma-Aldrich C270 Monoclonal Anti-Calpastatin antibody produced in mouse	Sigma-Aldrich C268 Monoclonal Anti-m-Calpain (Domain III/IV) antibody produced in mouse	Sigma-Aldrich C3545 Monoclonal Anti-Calmodulin antibody produced in mouse	Sigma-Aldrich P3088 Monoclonal Anti-Parvalbumin antibody produced in mouse
description clone 1F7E3D10, ascites fluid, buffered aqueous solution	description clone 107-82, ascites fluid	description clone 6D4, ascites fluid	description clone PARV-19, ascites fluid
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
species reactivity human, rat, bovine, pig	species reactivity pig, human, rat, bovine	species reactivity chicken, Dictyostelium discoideum, bovine, rat	species reactivity fish, frog, rabbit, canine, feline, bovine, pig, human, goat, rat
Gene Information human ... CAST(831) rat ... Cast(25403)	Gene Information human ... CAPN2(824) rat ... Capn2(29154)	Gene Information rat ... Calm1(24242)	Gene Information human ... PVALB(5816) rat ... Pvalb(25269)
biological source mouse	biological source mouse	biological source mouse	biological source mouse
antibody form ascites fluid	antibody form ascites fluid	antibody form ascites fluid	antibody form ascites fluid
clone 1F7E3D10, monoclonal	clone 107-82, monoclonal	clone 6D4, monoclonal	clone PARV-19, monoclonal

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Classe de armazenamento

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c