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Anti-Calbindin-D-28K (EG-20) antibody produced in rabbit

Name: Anti-Calbindin-D-28K (EG-20) antibody produced in rabbit
Brand: SIGMA
Price: 631 USD
Availability: InStock

affinity isolated antibody, buffered aqueous solution

Enhanced Validation

Synonym(s):

Anti-CALB, Anti-D-28K

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0.2 mL	Check Cart for Availability	$631.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.44

MDL number:

MFCD00164553

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IHC, WB

Citations:

$631.00

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Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 28 kDa

species reactivity

rat

enhanced validation

independent
Learn more about Antibody Enhanced Validation

technique(s)

immunohistochemistry: 1:2,000 using sections of rat cerebellum, western blot: 1:2,000 using a whole extract of rat brain

UniProt accession no.

P07171

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

rat ... Calb1(83839)

Description

General description

Calbindin-D-28K (CALB1) is a calcium binding protein in the brain. This 27kDa protein is located on human chromosome 8q21.

Calbindin-D-28k is an intracellular protein characterized by EF-hand type structural motifs that bind to Ca²⁺. This protein modulates synaptic plasticity and signal transduction . Anti-Calbindin-D-28K (EG-20) antibody recognizes rat calbindin-D-28K (28 kDa). Due to interspecies similarity, reactivity with human, mouse, bovine, chicken, porcine, and frog is expected, but has not been confirmed. Staining of calbindin-D-28K is specifically inhibited with the calbindin-D-28K immunizing peptide by immunoblotting.

Immunogen

The immunogen sequence is specific for calbindin-D-28K and is not found in other members of the EF-hand family such as calbindin-D-9K, calretinin, myosin light chain, parvalbumin, S-100a, S-100b, S100A2 (S100L) and S100A6 (calcyclin). This sequence is highly conserved across species, including human, mouse, bovine, chicken, porcine, and frog calbindin-D-28K sequences.

synthetic peptide corresponding to the C-terminal region of rat calbindin-D-28K (amino acids 225-244 with N-terminally added Lys-Gly) conjugated to KLH.

Application

Anti-Calbindin-D-28K (EG-20) antibody is suitable for use in immunohistochemistry (1:2,000 using sections of rat cerebellum), immunoblot and western blot (1:2,000 using a whole extract of rat brain). Enzymatic predigestion of formalin-fixed, paraffin-embedded sections by proteolytic enzymes (e.g., 0.1% trypsin or protease, 10 min., at RT or 37 ° C) improves immunohistochemical staining with the antibody.
Anti-Calbindin-D-28K (EG-20) antibody has been used in immunohistochemical (IHC) peroxidase staining and cupric silver staining. It has also been used in immunofluorescence.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Analysis Note

Enzymatic predigestion of formalin-fixed, paraffin-embedded sections by proteolytic enzymes (e.g., 0.1% trypsin or protease, 10 min at RT or 37 °C) improves immunohistochemical staining.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	C7354	SAB4200543	C9848
Sigma-Aldrich C2724 Anti-Calbindin-D-28K (EG-20) antibody produced in rabbit	Sigma-Aldrich C7354 Anti-Calbindin-D-28K (KD-15) antibody produced in rabbit	Sigma-Aldrich SAB4200543 Anti-Calbindin-D-28K antibody, Mouse monoclonal	Sigma-Aldrich C9848 Monoclonal Anti-Calbindin-D-28K antibody produced in mouse
description affinity isolated antibody, buffered aqueous solution	description affinity isolated antibody, buffered aqueous solution	description clone CB-955, purified from hybridoma cell culture	description clone CB-955, ascites fluid
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form purified immunoglobulin	antibody form ascites fluid
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
biological source rabbit	biological source rabbit	biological source mouse	biological source mouse
shipped in dry ice	shipped in dry ice	shipped in dry ice	shipped in dry ice
clone polyclonal	clone polyclonal	clone CB-955, monoclonal	clone CB-955, monoclonal

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Storage Class

12 - Non Combustible Liquids

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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