This product has been tested for solubility in chloroform at concentrations up to 100 mg/mL. For cell culture applications, it has been reported to dissolve in ethanol at 5 mg/mL (Ke et al., 2020). Based on this data, ethanol should also fully dissolve this product at 10 mM (~3.87 mg/mL).
C3045
Cholesterol
powder, BioReagent, suitable for cell culture, ≥99%
Synonym(s):
3β-Hydroxy-5-cholestene, 5-Cholesten-3β-ol
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 5 g | Available to ship onMarch 20, 2026fromMILWAUKEE | $149.00 | |
| 25 g | Available to ship onMarch 20, 2026fromMILWAUKEE | $583.00 | |
| 100 g | Available to ship onMarch 20, 2026fromMILWAUKEE | $1,560.00 |
About This Item
Empirical Formula (Hill Notation):
C27H46O
CAS Number:
Molecular Weight:
386.65
NACRES:
NA.75
PubChem Substance ID:
eCl@ss:
39023139
UNSPSC Code:
12352211
EC Number:
200-353-2
MDL number:
Beilstein/REAXYS Number:
1915888
biological source
ovine wool
Quality Level
product line
BioReagent
assay
≥99%
form
powder
technique(s)
cell culture | mammalian: suitable
bp
360 °C (lit.)
mp
147-149 °C (lit.)
solubility
H2O: 0.002 mg/mL, Tween: soluble, ethanol: soluble, serum: soluble
density
1.067 g/mL at 25 °C (lit.)
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This Item | |||
|---|---|---|---|
| biological source ovine wool | biological source sheep wool | biological source sheep wool | biological source - |
| technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) - | technique(s) HPLC: suitable, gas chromatography (GC): suitable |
| assay ≥99% | assay ≥92.5% (GC) | assay ≥99% | assay - |
| form powder | form powder | form powder | form powder |
| solubility H2O: 0.002 mg/mL, ethanol: soluble, Tween: soluble, serum: soluble | solubility water: soluble 0.00003 g/L at 20 °C | solubility water: soluble 0.00003 g/L at 20 °C | solubility water: soluble 0.00003 g/L at 20 °C |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in - |
General description
Cholesterol, a major sterol is the precursor for steroid hormones, bile acid and vitamin in mammals and coordinates endocytosis. It complexes with protein to form lipoproteins for transport in blood. It exists either as high-density lipoprotein (HDL) or low-density lipoprotein (LDL). HDL also called good cholesterol has less lipid content. LDL or bad cholesterol has high lipid content. Statin inhibition of HMG-CoA reductase enzyme in the cholesterol biosynthesis pathway controls elevated cholesterol levels in atherosclerosis and hypercholesterolemia. Low levels of cholesterol results in hypothyroidism, familial hypobetalipoproteinemia and liver disease. Imbalance of cholesterol homeostasis and intracellular cholesterol levels play a key role in cancer development. High cholesterol levels favor breast cancer progression.
Application
Cholesterol has been used:
- as a medium supplement in chorionic villous tissue culture form placenta
- as a component of hormone pellet for implantation in mice ovary
- for inducing expression of immunity-related GTPase M (IRGM) in human monocytic cell line, THP-1
Biochem/physiol Actions
Major component of all biological membranes; ~25% of total brain lipid is cholesterol.
related product
Product No.
Description
Pricing
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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The Crohn?s Disease Risk Factor IRGM Limits NLRP3 Inflammasome Activation by Impeding Its Assembly and by Mediating Its Selective Autophagy
Mehto S, et al.
Molecular Cell (2019)
Cholesterol biosynthesis: a mechanistic overview
Cerqueira NMFSA, et al.
Biochemistry, 55(39), 5483-5506 (2016)
Role of cholesterol in the development and progression of breast cancer
Llaverias G et al.
The American Journal of Pathology, 178(1), 402-412 (2011)
Impact of cholesterol on disease progression
Lin CJ, et al.
BioMedicine, 5(2) (2015)
Patient-derived xenograft model for uterine leiomyoma by sub-renal capsule grafting
Serna VA and Kurita T
Journal of biological methods, 5(2), 84-94 (2018)
Related Content
Global Trade Item Number
| SKU | GTIN |
|---|---|
| C3045-25G | 04061833485255 |
| C3045-100G | 04061833485248 |
| C3045-5G | 04061833485262 |
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How to prepare the BioReagent product, suitable for cell culture, ≥99% for cell culture? For example, I need 10mm/L of disintegrated cholesterol for an experiment. How can I dissolve it? In heated alcohol? Do you have a protocol for using cells?
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