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Monoclonal Anti-Cy3/Cy5−Biotin antibody produced in mouse

Name: Monoclonal Anti-Cy3/Cy5−Biotin antibody produced in mouse
Brand: SIGMA
Price: 677 USD
Availability: InStock

~1 mg/mL, clone CY-96, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Monoclonal Anti-Cy3/Cy5

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Size/SKU	Availability	Price
200 μL	Available to ship TODAYfromMILWAUKEE	$677.00

About This Item

NACRES:

NA.46

UNSPSC Code:

12352203

Conjugate:

biotin conjugate

Clone:

CY-96, monoclonal

Application:

ARR, DB, ELISA (d), ICC

Citations:

$677.00

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Properties

biological source

mouse

Quality Segment

200

conjugate

biotin conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

CY-96, monoclonal

form

buffered aqueous solution

concentration

~1 mg/mL

technique(s)

direct ELISA: suitable, dot blot: suitable, immunocytochemistry: 1-2 μg/mL using chicken fibroblasts, microarray: suitable

isotype

IgG2a

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

Monoclonal Anti-Cy3/Cy5 (mouse IgG2a isotype), Biotin conjugate is derived from the CY-96 hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a mixture of proteins labelled with cyanine 3 (Cy3) or Cy5. Cyanine dyes are ionized polymethine dyes.

Immunogen

mixture of proteins labeled with Cy3/Cy5.

Application

Monoclonal Anti-Cy3/Cy5 Biotin antibody produced in mouse has also been used in:

enzyme linked immunosorbent assay (ELISA)
immunocytochemistry
dot blot assay
protein microarrays
immunofluorescenc

Monoclonal Anti-Cy3/Cy5-Biotin antibody produced in mouse was used to label miR-107 in human breast cancer cell lines by confocal microscopy.

Biochem/physiol Actions

Cy dyes are pH insensitive fluorophores that are used to label, DNA, RNA and proteins in a variety of biological assays.

Cyanine dyes are small, soluble in aqueous solution and has tolerance to dimethyl sulfoxide (DMSO). They are more photostable than fluorescein, has high molar extinction coefficients and favourable quantum yields. Monoclonal antibodies to cyanine 3 (Cy3) and/or Cy5 are an important tool for Cy3/Cy5 signal amplification in assays where biomolecules are labelled with Cy3 or Cy5.

The antibody recognizes Cy3 and Cy5 conjugated to proteins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	C0992	V4014	C2571
Sigma-Aldrich C3117 Monoclonal Anti-Cy3/Cy5−Biotin antibody produced in mouse	Sigma-Aldrich C0992 Anti-Cy3/Cy5 antibody, Mouse monoclonal	Sigma-Aldrich V4014 Anti-V5-Cy3^™ antibody, Mouse monoclonal	Sigma-Aldrich C2571 Anti-Human IgG (Fc specific)-Cy3 antibody produced in goat
description ~1 mg/mL, clone CY-96, purified immunoglobulin, buffered aqueous solution	description clone CY-96, purified from hybridoma cell culture	description purified immunoglobulin, ~1 mg/mL, clone V5-10	description affinity isolated antibody, buffered aqueous solution
biological source mouse	biological source mouse	biological source mouse	biological source goat
antibody form purified immunoglobulin	antibody form purified from hybridoma cell culture	antibody form purified immunoglobulin	antibody form affinity isolated antibody
conjugate biotin conjugate	conjugate unconjugated	conjugate CY3 conjugate	conjugate CY3 conjugate
clone CY-96, monoclonal	clone CY-96, monoclonal	clone V5-10, monoclonal	clone polyclonal
form buffered aqueous solution	form buffered aqueous solution	form -	form buffered aqueous solution
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200

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Storage Class

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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