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C3175

Chloramphenicol-Water Soluble

powder, suitable for cell culture, BioReagent

Synonym(s):

Chloramphenicol, Chlornitromycin, Chlorocid, Chloromycetin, Cloramfenicol, D-(−)-threo-2-Dichloroacetamido-1-(4-nitrophenyl)-1,3-propanediol, D-(−)-threo-2,2-Dichloro-N-[β-hydroxy-α-(hydroxymethyl)-β-(4-nitrophenyl)ethyl]acetamide, Chloromycetin, D-threo-2,2-Dichloro-N-[β-hydroxy-α-(hydroxymethyl)-4-nitrophenethyl]aceta, Kloramfenikol

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.76
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Product Name

Chloramphenicol-Water Soluble, powder, BioReagent, suitable for cell culture

product line

BioReagent

Quality Level

form

powder

technique(s)

cell culture | mammalian: suitable

solubility

H2O: 50-500 mg/mL (stock solution), PBS: 50-500 mg/mL (stock solution), other salt solutions: not recommended

antibiotic activity spectrum

Gram-negative bacteria, Gram-positive bacteria, mycobacteria, mycoplasma

mode of action

protein synthesis | interferes

Application

Chloramphenicol-Water Soluble has been used:

  • as a supplement in Luria-Bertani (LB) medium to culture Escherichia coli[1]
  • as a supplement in LB agar to culture Citrobacter rodentium[2]
  • to culture Toxoplasma gondii[3]

Biochem/physiol Actions

Chloramphenicol is a natural product found in Streptomyces venezuelae.[4] It shows a broad range of antibiotic activity against Gram-negative and Gram-positive bacteria by binding to the peptidyl transferase loop of the ribosomal RNA to block the peptide bond formation.[5][6]

Packaging

Package size based on chloramphenicol

Other Notes

Formulated to contain approx. 100 mg chloramphenicol per gram of powder; balance 2-hydroxypropyl-β-cyclodextrin.
Keep container tightly closed in a dry and well-ventilated place. Light sensitive. Keep in a dry place.

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This Item
C1919PHR141231667
mode of action

protein synthesis | interferes

mode of action

protein synthesis | interferes

mode of action

-

mode of action

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | plant: suitable

technique(s)

-

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

antibiotic activity spectrum

Gram-negative bacteria, mycobacteria, Gram-positive bacteria, mycoplasma

antibiotic activity spectrum

Gram-negative bacteria, Gram-positive bacteria, mycobacteria, mycoplasma

antibiotic activity spectrum

-

antibiotic activity spectrum

-

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

100

product line

BioReagent

product line

BioReagent

product line

-

product line

VETRANAL®

form

powder

form

powder

form

-

form

-


pictograms

Health hazardCorrosion

signalword

Danger

Hazard Classifications

Carc. 2 - Eye Dam. 1 - Repr. 2

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type P2 (EN 143) respirator cartridges



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Articles

Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic


A Chatzitakis et al.
Water research, 42(1-2), 386-394 (2007-08-19)
The photocatalytic degradation of Chloramphenicol, an antibiotic drug, has been investigated in aqueous heterogeneous solutions containing n-type oxide semiconductors as photocatalysts. The disappearance of the organic molecule follows approximately a pseudo-first-order kinetics according to the Langmuir-Hinshelwood model. It was observed
D H Burke et al.
Chemistry & biology, 4(11), 833-843 (1998-01-31)
The problem of how macromolecules adopt specific shapes to recognize small molecules in their environment is readily addressed through in vitro selections (the SELEX protocol). RNA-antibiotic interactions are particularly attractive systems for study because they provide an opportunity to expand
Jaap C Hanekamp et al.
Environmental toxicology and pharmacology, 39(1), 213-220 (2014-12-22)
The antibiotic chloramphenicol (CAP) is banned from food production. Besides being a medicinal product, CAP is also a natural product, produced by Streptomyces Venezuelae. The lack of scientific data hampers setting of an Acceptable Daily Intake (ADI). Consequently, a maximum



Global Trade Item Number

SKUGTIN
C3175-100MG04061833485552

Questions

1–2 of 2 Questions  
  1. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  2. How do I calculate the molarity of product C3175, Chloramphenicol-Water Soluble?

    1 answer
    1. Product C3175 (Chloramphenicol-Water Soluble) is a complex of chloramphenicol and cyclodextrin. Cyclodextrin is used to aid in the solubility of chloramphenicol into water. In order to prepare a molar solution of chloramphenicol you will have to base it on the chloramphenicol content in the solution and not the entire complex.  Product C3175 is formulated to contain approximately 100 mg chloramphenicol per gram of powder.  Based on this average, if you are preparing a 500 mg/ml stock solution, there will be approximately 50 mg of chloramphenicol in 1 ml.  Below is the calculated molarity of chloramphenicol when the complex is dissolved at 500 mg/ml in water. molecular weight X unknown molarity X volume = complex weight X complex ratio323.13 g/mol X Molarity X 1 ml = 500 mg X 0.1           Molarity = 0.155 Mchloramphenicol molecular weight = 323.13 g/molmolarity (unknown) = mol/Lvolume = 1mlcomplex weight = 500 mgcomplex ratio = 100 mg chloramphenicol/1 gm complex

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