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MilliporeSigma

C3545

Monoclonal Anti-Calmodulin antibody produced in mouse

clone 6D4, ascites fluid

Synonym(s):

Anti-CALM3, Anti-calmodulin

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0.2 ML

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$611.00

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.44
MDL number:
MFCD00162206

Key Documents

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Product Name

Monoclonal Anti-Calmodulin antibody produced in mouse, clone 6D4, ascites fluid

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

6D4, monoclonal

contains

15 mM sodium azide as preservative

species reactivity

chicken, Dictyostelium discoideum, bovine, rat

technique(s)

western blot: 1:2,000 using a bovine brain calmodulin preparation

isotype

IgG1

UniProt accession no.

P0DP29

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

rat ... Calm1(24242)

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T6557C7464M4278
Monoclonal Anti-Calmodulin antibody produced in mouse clone 6D4, ascites fluid

Sigma-Aldrich

C3545

Monoclonal Anti-Calmodulin antibody produced in mouse

Anti-γ-Tubulin antibody, Mouse monoclonal clone GTU-88, ascites fluid

Sigma-Aldrich

T6557

Anti-γ-Tubulin antibody, Mouse monoclonal

Monoclonal Anti-Cyclin D1 antibody produced in mouse clone DCS-6, ascites fluid

Sigma-Aldrich

C7464

Monoclonal Anti-Cyclin D1 antibody produced in mouse

Monoclonal Anti-MAP1 antibody produced in mouse clone HM-1, ascites fluid

Sigma-Aldrich

M4278

Monoclonal Anti-MAP1 antibody produced in mouse

Gene Information

rat ... Calm1(24242)

Gene Information

human ... TUBG1(7283)
mouse ... Tubg1(103733)
rat ... Tubg1(252921)

Gene Information

human ... CCND1(595)
mouse ... Ccnd1(12443)
rat ... Ccnd1(58919)

Gene Information

mouse ... Mtap1a(17754)
rat ... Map1a(25152)

clone

6D4, monoclonal

clone

GTU-88, monoclonal

clone

DCS-6, monoclonal

clone

HM-1, monoclonal

species reactivity

chicken, Dictyostelium discoideum, bovine, rat

species reactivity

rat, hamster, chicken, human, bovine, canine, Xenopus, mouse

species reactivity

rat, mouse, human, monkey

species reactivity

mouse, rat

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

ascites fluid

Application

Monoclonal Anti-Calmodulin antibody produced in mouse has been used in:
  • enzyme-linked immunosorbent assay (ELISA)
  • immunoblot
  • dot blot
  • immunocytochemistry
  • immunoprecipitation
  • immunofluorescence[1]

Biochem/physiol Actions

Calmodulin is a ubiquitous molecule which can bind up to 4 calcium ions, regulating and mediating a wide variety of biochemical processes.
The antibody recognizes native and SDS-denatured calmodulin.
These antibodies are directed against different epitopes within the calmodulin molecule.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Calmodulin belongs to a family of structurally homologous Ca2+-binding proteins. CALM1 gene is located on human chromosome 14q32.11.
Calmodulin is a highly conserved 17kDa calcium binding protein found in all eukaryotic cells. It is a multifunctional molecule. Monoclonal anti-Calmodulin (mouse IgG1 isotype) is derived from the 6D4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with calmodulin, purified from Dictyostelium discoideum and conjugated to KLH.

Immunogen

preparation of purified calmodulin from Dictyostelium discoideum conjugated to KLH.

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000193766
0000179090
0000179090
0000193766
019M4880V

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C J Pallen et al.
The Journal of biological chemistry, 259(10), 6134-6141 (1984-05-25)
Calcineurin possesses phosphatase activity towards both protein (Stewart, A.A., Ingebritsen , T.S., Manalan , A., Klee , C.B., and Cohen, P. (1982) FEBS Lett. 137, 80-84) and nonprotein substrates ( Pallen , C.J., and Wang, J.H. (1983) J. Biol. Chem.
H C Lee et al.
The Journal of biological chemistry, 270(16), 9060-9066 (1995-04-21)
Cyclic ADP-ribose (cADPR) is emerging as an endogenous regulator of Ca2+-induced Ca2+ release (CICR), and we have recently demonstrated that its action is mediated by calmodulin (CaM) (Lee, H. C., Aarhus, R., Graeff, R., Gurnack, M. E., and Walseth, T.
Association between the polymorphisms of CALM1 gene and osteoarthritis risk: a meta-analysis based on observational studies
Yang H, et al.
Bioscience Reports, 38(5), BSR20181128-BSR20181128 (2018)
Identification of calmodulin and MlcC as light chains for Dictyostelium myosin-I isozymes
Crawley SW, et al.
Biochemistry, 50(30), 6579-6588 (2011)
The role of calmodulin and related proteins in plant cell function: an ever-thickening plot
Bergey DR, et al.
Springer Science Reviews, 2(1-2), 145-159 (2014)
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