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Monoclonal Anti-Calmodulin antibody produced in mouse

Name: Monoclonal Anti-Calmodulin antibody produced in mouse
Brand: SIGMA
Price: 519.35 CAD

clone 6D4, ascites fluid

Synonym(s):

Anti-CALM3, Anti-calmodulin

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Pack Size	SKU	Availability	Price
0.2 mL		Please contact Customer Service for Availability	$611.00 $519.35

About This Item

UNSPSC Code:

12352203

NACRES:

NA.44

MDL number:

MFCD00162206

Conjugate:

unconjugated

Clone:

6D4, monoclonal

Application:

Citations:

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Properties

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

6D4, monoclonal

contains

15 mM sodium azide as preservative

species reactivity

chicken, Dictyostelium discoideum, bovine, rat

technique(s)

western blot: 1:2,000 using a bovine brain calmodulin preparation

isotype

IgG1

UniProt accession no.

P0DP29

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

rat ... Calm1(24242)

Description

General description

Calmodulin belongs to a family of structurally homologous Ca²⁺-binding proteins. CALM1 gene is located on human chromosome 14q32.11.

Calmodulin is a highly conserved 17kDa calcium binding protein found in all eukaryotic cells. It is a multifunctional molecule. Monoclonal anti-Calmodulin (mouse IgG1 isotype) is derived from the 6D4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with calmodulin, purified from Dictyostelium discoideum and conjugated to KLH.

Immunogen

preparation of purified calmodulin from Dictyostelium discoideum conjugated to KLH.

Application

Monoclonal Anti-Calmodulin antibody produced in mouse has been used in:

enzyme-linked immunosorbent assay (ELISA)
immunoblot
dot blot
immunocytochemistry
immunoprecipitation
immunofluorescence[1]

Biochem/physiol Actions

Calmodulin is a ubiquitous molecule which can bind up to 4 calcium ions, regulating and mediating a wide variety of biochemical processes.

The antibody recognizes native and SDS-denatured calmodulin.

These antibodies are directed against different epitopes within the calmodulin molecule.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	C7464	M4278	C6344
Sigma-Aldrich C3545 Monoclonal Anti-Calmodulin antibody produced in mouse	Sigma-Aldrich C7464 Monoclonal Anti-Cyclin D1 antibody produced in mouse	Sigma-Aldrich M4278 Monoclonal Anti-MAP1 antibody produced in mouse	Sigma-Aldrich C6344 Monoclonal Anti-Connexin-32 antibody produced in mouse
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
biological source mouse	biological source mouse	biological source mouse	biological source mouse
antibody form ascites fluid	antibody form ascites fluid	antibody form ascites fluid	antibody form ascites fluid
Gene Information rat ... Calm1(24242)	Gene Information human ... CCND1(595) mouse ... Ccnd1(12443) rat ... Ccnd1(58919)	Gene Information mouse ... Mtap1a(17754) rat ... Map1a(25152)	Gene Information human ... GJB1(2705), GJB2(2706) mouse ... Gjb1(14618) rat ... Gja1(24392), Gjb1(29584)
clone 6D4, monoclonal	clone DCS-6, monoclonal	clone HM-1, monoclonal	clone CXN-32, monoclonal
species reactivity chicken, Dictyostelium discoideum, bovine, rat	species reactivity rat, mouse, human, monkey	species reactivity mouse, rat	species reactivity human, rat, mouse

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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A Proteomics Analysis of Calmodulin-Binding Proteins in Dictyostelium discoideum during the Transition from Unicellular Growth to Multicellular Development.

William D Kim et al.

International journal of molecular sciences, 22(4) (2021-02-13)

Calmodulin (CaM) is an essential calcium-binding protein within eukaryotes. CaM binds to calmodulin-binding proteins (CaMBPs) and influences a variety of cellular and developmental processes. In this study, we used immunoprecipitation coupled with mass spectrometry (LC-MS/MS) to reveal over 500 putative

Maximal stimulation-induced in situ myosin light chain kinase activity is upregulated in fetal compared with adult ovine carotid arteries

Injeti ER, et al.

American Journal of Physiology. Heart and Circulatory Physiology, 295(6), H2289-H2298 (2008)

The role of calmodulin and related proteins in plant cell function: an ever-thickening plot

Bergey DR, et al.

Springer Science Reviews, 2(1-2), 145-159 (2014)

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Global Trade Item Number

SKU	GTIN
C3545-.2ML	04061838104281

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