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Anti-phospho-β-Catenin (pSer³³/pSer³⁷) antibody, Mouse monoclonal

Name: Anti-phospho-β-Catenin (pSer<SUP>33</SUP>/pSer<SUP>37</SUP>) antibody, Mouse monoclonal
Brand: SIGMA
Price: 533.8 USD
Availability: InStock

clone BC-22, purified from hybridoma cell culture

Synonym(s):

Anti-CTNNB, Anti-EVR7, Anti-MRD19, Anti-NEDSDV, Anti-armadillo

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Size/SKU	Availability	Price
0.2 mL	Available to ship TODAYfromMILWAUKEE	$628.00 $533.80

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD03457566

Conjugate:

unconjugated

Clone:

BC-22, monoclonal

Application:

ARR, ELISA (i), WB

Citations:

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

BC-22, monoclonal

form

buffered aqueous solution

mol wt

antigen ~94 kDa

species reactivity

mouse, human, rat, chicken

concentration

~2 mg/mL

technique(s)

indirect ELISA: suitable, microarray: suitable, western blot: 5-10 μg/mL using whole cell extract of cultured human 293 cells treated with proteasome inhibitor MG132

isotype

IgG2b

UniProt accession no.

P35222

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pSer33/pSer37)

Gene Information

human ... CTNNB1(1499)
mouse ... Ctnnb1(12387)
rat ... Ctnnb1(84353)

Description

General description

CTNNB1 (catenin β 1) gene is mapped to human chromosome 3p21. The encoded protein β-catenin is localized to the nucleus.

Immunogen

synthetic phosphopeptide corresponding to the region that contains serines 33 and 37 of human β-catenin.

Application

Monoclonal Anti-phospho-β-Catenin (pSer33/pSer37) antibody is suitable for:

indirect ELISA
microarray
western blot at a dilution of 5-10μg/mL using whole cell extract of cultured human 293 cells treated with proteasome inhibitor MG132.

Biochem/physiol Actions

β-catenin is mapped on the short arm of chromosome 3. It is useful for differential diagnosis of Osteoblastoma (OB) and osteosarcoma (OS). β-catenin signaling pathway may contribute to cholangiocarcinoma (CCA) cell proliferation. It may serve as a prognostic marker for CCA progression and hence can be significant in the CCA therapy target.
In Wnt/β-catenin signal pathway β-Catenin participates as a signal transduction molecule. The pathway is associated with development, tissue homeostasis and oncogenic transcription. Upregulation of the gene is associated with glioma cell proliferation. The protein encoded has dual function that regulates the coordination of cell-cell adhesion by linking cytoskeleton and cellular junctions and gene transcription. Wnt/β-catenin signal pathway is known to induce progression colon cancer progression.

The antibody is inhibited more efficiently by the pSer³⁷ peptide than by the pSer³³peptide and is not inhibited by the nonphosphorylated peptide. It does not detect the unphosphorylated or the Ser³³ phosphorylated protein and does not recognize phosphorylated plakoglobin.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	C7082	C2206	SAB4504348
Sigma-Aldrich C4231 Anti-phospho-β-Catenin (pSer³³/pSer³⁷) antibody, Mouse monoclonal	Sigma-Aldrich C7082 Monoclonal Anti-β-Catenin antibody produced in mouse	Sigma-Aldrich C2206 Anti-β-Catenin antibody produced in rabbit	Sigma-Aldrich SAB4504348 Anti-phospho-Catenin-β (pSer³⁷) antibody produced in rabbit
description clone BC-22, purified from hybridoma cell culture	description clone 6F9, ascites fluid	description whole antiserum	description affinity isolated antibody
antibody form purified immunoglobulin	antibody form ascites fluid	antibody form whole antiserum	antibody form affinity isolated antibody
biological source mouse	biological source mouse	biological source rabbit	biological source rabbit
species reactivity mouse, human, rat, chicken	species reactivity human, chicken, canine, bovine	species reactivity several mammalian species	species reactivity mouse, rat, human
clone BC-22, monoclonal	clone 6F9, monoclonal	clone polyclonal	clone polyclonal
Gene Information human ... CTNNB1(1499) mouse ... Ctnnb1(12387) rat ... Ctnnb1(84353)	Gene Information human ... CTNNB1(1499)	Gene Information human ... CTNNB1(1499) mouse ... Ctnnb1(12387) rat ... Ctnnb1(84353)	Gene Information human ... CTNNB1(1499)
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated

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Storage Class

10 - Combustible liquids

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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