This is a product of PromoCell. Please see the instructions for use provided by the manufacturer:
1. Fill Lymphocyte Separation Medium 1077 in a tube. Use 3 ml for a 15 ml tube and 20 ml for a 50 ml tube.
2. Hold the tube at a 45° angle and carefully load the cell suspension to be separated on top of the separation medium.
Note: Perform this step slowly but constantly. Take care that the two phases do not mix and a sharp border is present.
For best results, use an electrical pipet aid with EX-function allowing the slow and constant efflux of the cell suspension from the pipet.
3. Centrifuge the tube at 440 x g for 40 minutes at room temperature without brake.
4. Carefully aspirate most of the supernatant without disturbing the layer of mononuclear cells in the interphase.
5. Aspirate the ring of mononuclear cells from the interphase using a pipet. Keep the volume as small as possible.
6. Wash the mononuclear cells with PBS/0.1% HSA or BSA and centrifuge at 360 x g for 10 minutes.
7. Wash the mononuclear cells with PBS/0.1% HSA or BSA and centrifuge at 200 x g for 10 minutes X 2
8. The mononuclear cell preparation is ready.
C-44010
Lymphocyte Separation Medium 1077
500 ml
Synonym(s):
Lymphocyte Medium, Separation Medium for Lymphocytes
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 500 mL | Please contact Customer Service for Availability | $173.00 |
About This Item
form
aqueous solution
packaging
pkg of 500 ml
technique(s)
cell culture | mammalian: suitable
shipped in
ambient
storage temp.
room temp
General description
Application
Preparation Note
Analysis Note
1 of 1
This Item | |||
|---|---|---|---|
| form aqueous solution | form liquid | form liquid | form liquid |
| technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) cell culture | mammalian: suitable | technique(s) - |
| shipped in ambient | shipped in wet ice | shipped in wet ice | shipped in wet ice |
| storage temp. room temp | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| packaging pkg of 500 ml | packaging - | packaging pkg of 500 ml | packaging - |
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Storage Class
12 - Non Combustible Liquids
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Articles
Umbilical cord blood and adult peripheral blood mononuclear cells with optimized maintenance medium for short-term cultivation.
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What's the procedure of lymphocytes separation with this media and the quantity used .
1 answer-
Helpful?
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What percentage of lymphocytes/monocytes, etc. would be expected if they use it on fresh human blood?
1 answer-
When utilizing the Lymphocyte Separation Medium 1077 (cat. #C-44010) on fresh human whole blood, the expected percentage of lymphocytes/monocytes typical for this sample is usually in the range of 70-90% of the enriched PBMCs.
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