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C4407

Human Collagen Type III

from human placenta, powder, suitable for cell culture

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1 mg

Available to ship TODAYfromMILWAUKEE

$206.00
5 mg

Available to ship TODAYfromMILWAUKEE

$641.00
25 mg

Estimated to ship onOctober 06, 2026fromMILWAUKEE

$3,390.00

About This Item

CAS Number:
UNSPSC Code:
12352202
NACRES:
NA.32
EC Number:
232-697-4
MDL number:
Form:
powder
Assay:
≥90% (GE)
Biological source:
human placenta

$206.00


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Product Name

Collagen from human placenta, Bornstein and Traub Type III (Sigma Type X), powder

biological source

human placenta

Quality Segment

assay

≥90% (GE)

form

powder

technique(s)

cell culture | mammalian: suitable

impurities

HIV, hepatitis B and hepatitis C, none detected

solubility

aqueous acid: soluble

UniProt accession no.

storage temp.

2-8°C

Gene Information

human ... COL3A1(1300)

Application

Collagen from human placenta has been used to assess the bioelectric effects of quinine on airway epithelial cells, to study the selective toxicity of engineered lentvirus lytic peptides and in a particle aggregation assay for the rapid detection of fibronectin, fibrinogen and collagen receptors on Staphylococcus aureus. It has also been used in a magnesium-dependent, collagen-binding assay during characterization of human lung tumor-associated antigens.

Preparation Note

This product was prepared by a modification of the pepsin extraction and salt fractionation method of Hill, R.J. and Harper, E., Anal. Biochem., 141 (1984). It is a lyophilized powder that can be reconstituted in sterile .5 M acetic acid, PBS or water at 1 mg/mL. A PBS solution will be stable for at least one year at -20°C.

Analysis Note

An SDS polyacrylamide gel electrophoresis test run under reducing conditions consistent with basement membrane collagen yields three major bands.

Other Notes

All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue.
Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Be wary of confusing Sigma-type designations with recognized collagen classification types.

Disclaimer

This product should be stored at 2-8 °C and will retain activity in these conditions for 2 years.

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This Item
C5533234149C5608
description

Bornstein and Traub Type III (Sigma Type X), powder

description

Bornstein and Traub Type IV, powder, BioReagent, suitable for cell culture

description

(250 μg), solution, purified by (Highly purified), Native protein, suitable for cell culture

description

Bornstein and Traub Type I, powder

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | stem cell: suitable

biological source

human placenta

biological source

human placenta

biological source

-

biological source

rabbit skin

assay

≥90% (GE)

assay

-

assay

≥90% (SDS-PAGE)

assay

-

form

powder

form

powder

form

solution

form

powder

solubility

aqueous acid: soluble

solubility

acetic acid: 0.5-2.0 mg/mL (Dissolve for several hours at 2-8 °C, occasionally swirling.)

solubility

-

solubility

aqueous acid: ≤10 mg/mL, aqueous sodium acetate buffer: soluble (0.01-0.5M)

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C


Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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