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CD152/Fc Chimera, Cytolytic from mouse

Name: CD152/Fc Chimera, Cytolytic from mouse
Brand: SIGMA
Price: 592 EUR

recombinant, expressed in NS.1 cells, buffered aqueous solution

Synonym(s):

CTLA4/Fc Chimera, cytolytic

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Pack Size	SKU	Availability	Price
1 mg		Check Cart for Availability	€ 592,00

About This Item

UNSPSC Code:

51111800

NACRES:

NA.77

MDL number:

MFCD01092888

Form:

buffered aqueous solution

Assay:

≥99% (SDS-PAGE)

Biological source:

mouse

Recombinant:

expressed in NS.1 cells

Mol wt:

97 kDa

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Properties

biological source

mouse

Quality Level

200

recombinant

expressed in NS.1 cells

assay

≥99% (SDS-PAGE)

form

buffered aqueous solution

mol wt

97 kDa

packaging

pkg of 1 mg

storage condition

avoid repeated freeze/thaw cycles

impurities

≤1 EU/mg protein Endotoxin level (LAL test)

UniProt accession no.

P09793

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... Cd152(12477), Ctla4(12477)

Description

General description

The complement (C1q) and FcγR I binding sites of the Fcγ2a fragment allow the Fc portion of the fusion protein to effectively facilitate direct antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC).

Biochem/physiol Actions

CD152 (CTLA4), a cell surface glycoprotein expressed at low levels on activated T cells, is a high affinity receptor for the costimulatory molecules CD80 (B7-1) and CD86 (B7-2). A related cell surface glycoprotein, CD28, binds to CD80 and CD86 with lower affinity. The soluble CD152/Fc chimeric fusion protein blocks the B7/CD28 signaling pathway by binding to CD80 and CD86.

Physical form

Solution, 0.2 μm filtered, in phosphate buffered saline, pH 7.4, with no preservative added.

Preparation Note

Purified from serum-free tissue culture supernatant

Other Notes

The extracellular domain (160 amino acids) of mouse CD152 is fused to mouse IgG2a Fc domain.

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This Item	E9902	E0778	R0775
Sigma-Aldrich C4483 CD152/Fc Chimera, Cytolytic from mouse	Sigma-Aldrich E9902 Ephrin-A1/Fc Chimera from mouse	Sigma-Aldrich E0778 Ephrin-B2/Fc Chimera from mouse	Sigma-Aldrich R0775 RANK/Fc Chimera from mouse
biological source mouse	biological source mouse	biological source mouse	biological source mouse
recombinant expressed in NS.1 cells	recombinant expressed in NSO cells	recombinant expressed in NSO cells	recombinant expressed in NSO cells
assay ≥99% (SDS-PAGE)	assay >90% (SDS-PAGE)	assay >90% (SDS-PAGE)	assay ≥95% (SDS-PAGE)
mol wt 97 kDa	mol wt monomer calculated mol wt 46.8 kDa, 50-55 kDa by SDS-PAGE (reducing)	mol wt monomer calculated mol wt 49.6 kDa, 60-65 kDa by SDS-PAGE (reducing)	mol wt 60 kDa
form buffered aqueous solution	form lyophilized powder	form lyophilized powder	form lyophilized powder
Gene Information mouse ... Cd152(12477), Ctla4(12477)	Gene Information mouse ... Efna1(13636)	Gene Information mouse ... Efnb2(13642)	Gene Information mouse ... Tnfrsf11a(21934)

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Recent advances using anti-CTLA-4 for the treatment of melanoma.

Amod A Sarnaik et al.

Cancer journal (Sudbury, Mass.), 15(3), 169-173 (2009-06-27)

Metastatic melanoma is a disease associated with poor prognosis, with a median survival reported to range from 6 to 9 months. Patients who are not candidates for surgical resection have an even worse expected survival. This is largely due to

Specific subsets of murine dendritic cells acquire potent T cell regulatory functions following CTLA4-mediated induction of indoleamine 2,3 dioxygenase.

Andrew L Mellor et al.

International immunology, 16(10), 1391-1401 (2004-09-08)

Murine dendritic cells (DCs) expressing indoleamine 2,3 dioxygenase (IDO) catabolize tryptophan and can suppress T cell responses elicited in vivo. Here, we identify specific subsets of splenic (CD11c+) dendritic cells competent to mediate IDO-dependent T cell suppression following CTLA4-mediated ligation

Cutting edge: induced indoleamine 2,3 dioxygenase expression in dendritic cell subsets suppresses T cell clonal expansion.