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C4483

CD152/Fc Chimera, Cytolytic from mouse

recombinant, expressed in NS.1 cells, buffered aqueous solution

Synonym(s):

CTLA4/Fc Chimera, cytolytic

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1 mg
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$921.00

About This Item

UNSPSC Code:
51111800
NACRES:
NA.77
MDL number:
Form:
buffered aqueous solution
Assay:
≥99% (SDS-PAGE)
Biological source:
mouse
Recombinant:
expressed in NS.1 cells
Mol wt:
97 kDa

$921.00


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biological source

mouse

Quality Segment

recombinant

expressed in NS.1 cells

assay

≥99% (SDS-PAGE)

form

buffered aqueous solution

mol wt

97 kDa

packaging

pkg of 1 mg

storage condition

avoid repeated freeze/thaw cycles

impurities

≤1 EU/mg protein Endotoxin level (LAL test)

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

General description

The complement (C1q) and FcγR I binding sites of the Fcγ2a fragment allow the Fc portion of the fusion protein to effectively facilitate direct antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC).

Biochem/physiol Actions

CD152 (CTLA4), a cell surface glycoprotein expressed at low levels on activated T cells, is a high affinity receptor for the costimulatory molecules CD80 (B7-1) and CD86 (B7-2). A related cell surface glycoprotein, CD28, binds to CD80 and CD86 with lower affinity. The soluble CD152/Fc chimeric fusion protein blocks the B7/CD28 signaling pathway by binding to CD80 and CD86.

Physical form

Solution, 0.2 μm filtered, in phosphate buffered saline, pH 7.4, with no preservative added.

Preparation Note

Purified from serum-free tissue culture supernatant

Other Notes

The extracellular domain (160 amino acids) of mouse CD152 is fused to mouse IgG2a Fc domain.

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This Item
I9905E9902E2153
recombinant

expressed in NS.1 cells

recombinant

expressed in CHO cells

recombinant

expressed in NSO cells

recombinant

expressed in NSO cells

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

assay

≥99% (SDS-PAGE)

assay

>98% (SDS-PAGE)

assay

>90% (SDS-PAGE)

assay

>90% (SDS-PAGE)

form

buffered aqueous solution

form

solution

form

lyophilized powder

form

lyophilized powder

mol wt

97 kDa

mol wt

94 kDa

mol wt

monomer calculated mol wt 46.8 kDa, 50-55 kDa by SDS-PAGE (reducing)

mol wt

monomer calculated mol wt 88.2 kDa, ~120 kDa by SDS-PAGE (reducing)

UniProt accession no.

P09793

UniProt accession no.

P04351

UniProt accession no.

P20827

UniProt accession no.

P09803


Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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