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Anti-Calreticulin antibody produced in rabbit

Name: Anti-Calreticulin antibody produced in rabbit
Brand: SIGMA
Price: 646.85 CAD

IgG fraction of antiserum, buffered aqueous solution

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Pack Size	SKU	Availability	Price
0.2 mL		Please contact Customer Service for Availability	$761.00 $646.85

About This Item

UNSPSC Code:

12352203

NACRES:

NA.43

MDL number:

MFCD01092536

Conjugate:

unconjugated

Clone:

polyclonal

Application:

ARR, IF, IP, WB

Citations:

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Properties

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 50 kDa

species reactivity

human, canine

technique(s)

immunoprecipitation (IP): suitable using whole cell RIPA lysate of the human epitheloid carcinoma HeLa cell line, indirect immunofluorescence: 1:100 using 3% paraformaldehyde-fixed, 0.5% Triton X-100 treated, Madin Darby canine kidney MDCK cell line, microarray: suitable, western blot: 1:2,000 using whole cell RIPA lysate of the human epitheloid carcinoma HeLa cell line

UniProt accession no.

P27797

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CALR(811)

Description

General description

The gene for calreticulin (CALR) is located on the human chromosome 19p13.13. The encoded protein is a predominantly conserved calcium-binding chaperone, which is localized primarily to the lumen of the endoplasmic reticulum (ER). Calreticulin comprises three domains, a globular N-domain, a proline-rich P-domain, and a highly acidic C-domain.

Immunogen

synthetic peptide corresponding to the C-terminus of human calreticulin (amino acids 401-417).

Application

Anti-Calreticulin antibody produced in rabbit has been used in:

immunoblotting[1]
immunofluorescence[2]
immunocytochemistry{91
immunoprecipitation

Biochem/physiol Actions

Anti-Calreticulin recognizes human and dog calreticulin (55-60 kDa).

Calreticulin facilitates transient interaction with newly synthesized cellular and extracellular proteins for folding and assembling in the endoplasmic reticulum (ER) before its localization to the cytosol or cell surface. Calreticulin acts as a lectin-like chaperone binding oligosaccharide residues of newly synthesized N-linked glycoproteins and misfolded proteins. It is believed to play a critical role in quality control processes during protein synthesis and folding and calcium (Ca²⁺) homeostasis. Increased expression of calreticulin increases the Ca²⁺ storage capacity of the ER. It also appears to modulate store-operated Ca²⁺-influx and to alter Ca²⁺ transport by the sarcoplasmic/ER Ca²⁺-adenosine triphosphatase (ATPase) (SERCA). Overexpression of calreticulin results in increased sensitivity of HeLa cells to drug-induced apoptosis. However, increased resistance to apoptosis has been observed in calreticulin-deficient cells.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Store at -20 °C. For continuous use, the product maybe stored at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots at-20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded ifnot used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	C7492	C3237	G8918
$Anti-Calreticulin antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich C4606 Anti-Calreticulin antibody produced in rabbit	Sigma-Aldrich C7492 Monoclonal Anti-Calreticulin antibody produced in mouse	$Anti-Caveolin-1 antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich C3237 Anti-Caveolin-1 antibody produced in rabbit	$Anti-GRP78/BiP (GL-19) antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich G8918 Anti-GRP78/BiP (GL-19) antibody produced in rabbit
biological source rabbit	biological source mouse	biological source rabbit	biological source rabbit
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
antibody form IgG fraction of antiserum	antibody form tissue culture supernatant	antibody form IgG fraction of antiserum	antibody form IgG fraction of antiserum
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
technique(s) immunoprecipitation (IP): suitable using whole cell RIPA lysate of the human epitheloid carcinoma HeLa cell line, microarray: suitable, indirect immunofluorescence: 1:100 using 3% paraformaldehyde-fixed, 0.5% Triton X-100 treated, Madin Darby canine kidney MDCK cell line, western blot: 1:2,000 using whole cell RIPA lysate of the human epitheloid carcinoma HeLa cell line	technique(s) flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, indirect ELISA: suitable, western blot: 1:50-1:100 using total cell extract of HeLa cells	technique(s) indirect immunofluorescence: 1:500 using mouse fibroblast NIH3T3 cell line, microarray: suitable, western blot: 1:2,000 using whole cell extract of the human umbilical vein endothelial HUVEC cell line and the rat fibroblast Rat-1 cell line	technique(s) immunocytochemistry: 1:1,000 using mouse fibroblasts NIH3T3 cells, microarray: suitable, western blot: 1:3,000 using human epitheloid carcinoma HeLa whole cell extract

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Clinical and hematological relevance of JAK2 V617F and CALR mutations in BCR-ABL-negative ET patients.

N Limsuwanachot et al.

Hematology (Amsterdam, Netherlands), 22(10), 599-606 (2017-04-14)

Classical BCR-ABL1-negative myeloproliferative neoplasms (MPNs) including polycythemia vera, essential thrombocythemia (ET), and primary myelofibrosis frequently harbor JAK2, MPL, and CALR somatic mutations. AS-PCR for JAK2 V617F, pyrosequencing for MPL W515L/K, and PCR-fragment analysis for CALR exon 9 mutations were established

ELOVL5 mutations cause spinocerebellar ataxia 38.

Eleonora Di Gregorio et al.

American journal of human genetics, 95(2), 209-217 (2014-07-30)

Spinocerebellar ataxias (SCAs) are a heterogeneous group of autosomal-dominant neurodegenerative disorders involving the cerebellum and 23 different genes. We mapped SCA38 to a 56 Mb region on chromosome 6p in a SCA-affected Italian family by whole-genome linkage analysis. Targeted resequencing

Calreticulin, a multi-process calcium-buffering chaperone of the endoplasmic reticulum.

Marek Michalak et al.

The Biochemical journal, 417(3), 651-666 (2009-01-13)

Calreticulin is an ER (endoplasmic reticulum) luminal Ca2+-buffering chaperone. The protein is involved in regulation of intracellular Ca2+ homoeostasis and ER Ca2+ capacity. The protein impacts on store-operated Ca2+ influx and influences Ca2+-dependent transcriptional pathways during embryonic development. Calreticulin is

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Global Trade Item Number

SKU	GTIN
C4606-.2ML	04061838104335

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