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Monoclonal Anti-Cytokeratin Peptide 8 antibody produced in mouse

Name: Monoclonal Anti-Cytokeratin Peptide 8 antibody produced in mouse
Brand: SIGMA
Price: 643.45 CAD

clone M20, ascites fluid

Synonym(s):

Anti-CARD2, Anti-CK-8, Anti-CK8, Anti-CYK8, Anti-K2C8, Anti-K8, Anti-KO

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Pack Size	SKU	Availability	Price
100 μL		Please contact Customer Service for Availability	$757.00 $643.45
0.2 mL		Please contact Customer Service for Availability	$981.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD00162254

Conjugate:

unconjugated

Clone:

M20, monoclonal

Application:

IF, IHC (f), WB

Citations:

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Properties

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

M20, monoclonal

mol wt

antigen 52 kDa

contains

15 mM sodium azide

species reactivity

feline, human, rabbit, canine, bovine

technique(s)

immunohistochemistry (frozen sections): suitable, indirect immunofluorescence: 1:200 using frozen sections of human or animal tissue, western blot: suitable

isotype

IgG1

UniProt accession no.

P05787

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... KRT8(3856)

Description

General description

Cytokeratin is a 52.5kD cytoplasmic structural protein that belongs to type II, neutral-to-basic subfamily. It consists of 29 different proteins which is the characteristic of epithelial and trichocytic cells. Monoclonal anti-cytokeratin peptide 8 antibody can be used as a marker for epithelial cell differentiation as well as a tool for tumor identification and classification. It can also be used in western blotting. Mouse anti-cytokeratin peptide 8 antibody reacts specifically with various human simple and complex epithelia like- liver, intestine, pancreas, urinary bladder, salivary gland, thyroid, prostate, mesothel, and placenta but not with stratified squamous epithelia. The product also does not react with non-epithelial tissues (exceptions-certain smooth muscle cells). This product has also shown cross reactivity for cytokeratin of rabbit, cow, dog, and cat.Monoclonal Anti-Cytokeratin 8 (mouse IgG1 isotype) is derived from the M20 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a cytokeratin preparation purified from the human breast carcinoma cell line MCF7.

Immunogen

cytokeratin from human breast carcinoma cell line MCF7.

Application

Monoclonal anti-cytokeratin peptide 8 antibody has been used in

immunofluorescence microscopy
immunohistochemistry
dot blotting
protein preparation from bovine epithelial cells
immunofluorescence[1]
western blotting

Biochem/physiol Actions

Monoclonal anti-cytokeratin peptide 8 antibody can be used as a marker for epithelial cell differentiation as well as a tool for tumor identification and classification. It can also be used in western blotting.

Mouse anti-cytokeratin peptide 8 antibody reacts specifically with various human simple and complex epithelia like- liver, intestine, pancreas, urinary bladder, salivary gland, thyroid, prostate, mesothel, and placenta but not with stratified squamous epithelia. The product also does not react with non-epithelial tissues (exceptions-certain smooth muscle cells). This product has also shown cross reactivity for cytokeratin of rabbit, cow, dog, and cat.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	C6417	SAB4700777	C1801
Sigma-Aldrich C5301 Monoclonal Anti-Cytokeratin Peptide 8 antibody produced in mouse	Sigma-Aldrich C6417 Monoclonal Anti-Cytokeratin Peptide 7 antibody produced in mouse	Sigma-Aldrich SAB4700777 Monoclonal Anti-Cytokeratin 8 antibody produced in mouse	Sigma-Aldrich C1801 Monoclonal Anti-Cytokeratin, pan antibody produced in mouse
Gene Information human ... KRT8(3856)	Gene Information human ... KRT7(3855)	Gene Information human ... KRT8(3856)	Gene Information bovine ... Krt1(100301161) dog ... Krt1(444857) human ... KRT1(3848), KRT5(3852), KRT5(3852), KRT5(3852), KRT6A(3868), KRT6A(3868), KRT6A(3868), KRT6B(3854), KRT6B(3854), KRT6B(3854), KRT8(3856), KRT8(3856), KRT8(3856) mouse ... KRT1(16678), KRT1(16678), KRT1(16678), Krt1(16678), Krt5(110308), Krt5(110308), Krt5(110308), Krt6a(16687), Krt6a(16687), Krt6a(16687), Krt6b(16688), Krt6b(16688), Krt6b(16688), Krt8(16691), Krt8(16691), Krt8(16691) rat ... Krt1(300250), Krt2-5(369017), Krt2-5(369017), Krt2-5(369017), Krt2-8(25626), Krt2-8(25626), Krt2-8(25626)
clone M20, monoclonal	clone LDS-68, monoclonal	clone C-51, monoclonal	clone PCK-26, monoclonal
antibody form ascites fluid	antibody form ascites fluid	antibody form purified immunoglobulin	antibody form ascites fluid
species reactivity feline, human, rabbit, canine, bovine	species reactivity human	species reactivity sheep, pig, human, bovine	species reactivity human, chicken, snake, hamster, pig, goat, feline, bovine, carp, rat, rabbit, canine, lizard, sheep, mouse, guinea pig
biological source mouse	biological source mouse	biological source mouse	biological source mouse
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

0000496195

0000490046

0000095050

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Global Trade Item Number

SKU	GTIN
C5301-.2ML	04061838104373
C5301-100UL	04061838030467

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