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C5421

Cholesterol Oxidase from microorganisms

aqueous solution, ≥30 units/mg protein (biuret)

Synonym(s):

Cholesterol:oxygen oxidoreductase

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100 units
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$269.00
500 units
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$1,040.00

About This Item

CAS Number:
9028-76-6
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-842-1
MDL number:
MFCD00130783
EC Number:
1.1.3.6 (BRENDA, IUBMB)
Specific activity:
≥30 units/mg protein (biuret)

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form

aqueous solution

Quality Segment

200

specific activity

≥30 units/mg protein (biuret)

mol wt

62 kDa

solubility

50 mM potassium phosphate buffer, pH 7.0: soluble (Cold)

shipped in

dry ice

storage temp.

−70°C

Application

Cholesterol oxidase has been used in a study to improve treatment of oxysterol-mediated cytotoxicity. Cholesterol oxidase has also been used in a study that evaluated the effects of cholesterol depletion. This study reported that cholesterol depletion impairs the coupling between channel opening and vesicle release. Additionally, cholesterol oxidase is used to determine serum cholesterol levels. The enzyme also finds application in the microanalysis of steroids in food samples. It has also been used for distinguishing 3-ketosteroids from 3β-hydroxysteroids. Transgenic plants expressing cholesterol oxidase have been investigated in the fight against the cotton boll weevil. CHOD has also been used as a molecular probe to elucidate cellular membrane structures.
Cholesterol oxidase has been used in a study to improve treatment of oxysterol-mediated cytotoxicity. Cholesterol oxidase has also been used in a study that determined that cholesterol depletion impairs coupling between channel opening and vesicle release by allowing voltage-gated calcium channels to move further from release sites.

Biochem/physiol Actions

Cholesterol oxidase (CHOD) is a monomeric flavoprotein containing FAD that catalyzes the first step in cholesterol catabolism. This bifunctional enzyme oxidizes cholesterol to cholest-5-en-3-one in an FAD-requiring step. The pH optimum of the enzyme is 7.0 to 7.5 and temperature optimum is 50 °C. The pH stability is 5.7-7.8. Hg2+, Ag+, and ionic detergents inhibit the enzyme activity. Molecular mass of the enzyme is 62 kDa. Pathogenic bacteria require CHOD to infect a host′s macrophage.

Preparation Note

CHOD is soluble in cold 50 mM potassium phosphate buffer, pH 7.0. Prepare solutions immediately before use.

Other Notes

One unit will convert 1.0 μmole of cholesterol to 4-cholesten-3-one per min at pH 7.5 at 25 °C. Note: 4-cholesten-3-one may undergo isomerization.

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Cholesterol Oxidase from microorganisms aqueous solution, ≥30 units/mg protein (biuret)

Sigma-Aldrich

C5421

Cholesterol Oxidase from microorganisms

Cholesterol Oxidase from microorganisms lyophilized powder, ≥50 units/mg protein, recombinant, expressed in E. coli

Sigma-Aldrich

C8868

Cholesterol Oxidase from microorganisms

Cholesterol Esterase from Pseudomonas fluorescens lyophilized powder, ≥10,000 units/g protein

Sigma-Aldrich

C9281

Cholesterol Esterase from Pseudomonas fluorescens

Cholesterol Esterase, Microbial Cholesterol Esterase, Microbial, CAS 9026-00-0, is a native enzyme that catalyzes hydrolysis and formation of cholesterol esters, ranging from cholesterol acetate to cholesterol stearate.

Sigma-Aldrich

228180

Cholesterol Esterase, Microbial

description

aqueous solution, ≥30 units/mg protein (biuret)

description

lyophilized powder, ≥50 units/mg protein, recombinant, expressed in E. coli

description

lyophilized powder, ≥10,000 units/g protein

description

Cholesterol Esterase, Microbial, CAS 9026-00-0, is a native enzyme that catalyzes hydrolysis and formation of cholesterol esters, ranging from cholesterol acetate to cholesterol stearate.

specific activity

≥30 units/mg protein (biuret)

specific activity

≥50 units/mg protein

specific activity

≥10,000 units/g protein

specific activity

≥100 units/mg protein, ≥70 units/mg dry wt.

form

aqueous solution

form

lyophilized powder

form

lyophilized powder

form

powder

mol wt

62 kDa

mol wt

64 kDa

mol wt

~129 kDa

mol wt

-

shipped in

dry ice

shipped in

-

shipped in

-

shipped in

ambient

solubility

50 mM potassium phosphate buffer, pH 7.0: soluble (Cold)

solubility

-

solubility

0.4 M potassium phosphate, pH 7.0: soluble 1.0 mg/mL

solubility

10 mM potassium phosphate/sodium hydroxide, 0.1% BSA, pH 7.5: soluble

storage temp.

−70°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

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hcodes

H334

pictograms

Health hazard
GHS08

signalword

Danger

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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