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C5421

Cholesterol Oxidase from microorganisms

aqueous solution, ≥30 units/mg protein (biuret)

Synonym(s):

Cholesterol:oxygen oxidoreductase

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100 units
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$279.00
500 units
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$1,080.00

About This Item

CAS Number:
9028-76-6
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-842-1
MDL number:
MFCD00130783
EC Number:
1.1.3.6 (BRENDA, IUBMB)
Specific activity:
≥30 units/mg protein (biuret)

$279.00

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form

aqueous solution

Quality Segment

specific activity

≥30 units/mg protein (biuret)

mol wt

62 kDa

solubility

50 mM potassium phosphate buffer, pH 7.0: soluble (Cold)

shipped in

dry ice

storage temp.

−70°C

Application

Cholesterol oxidase has been used in a study to improve treatment of oxysterol-mediated cytotoxicity. Cholesterol oxidase has also been used in a study that evaluated the effects of cholesterol depletion. This study reported that cholesterol depletion impairs the coupling between channel opening and vesicle release. Additionally, cholesterol oxidase is used to determine serum cholesterol levels. The enzyme also finds application in the microanalysis of steroids in food samples. It has also been used for distinguishing 3-ketosteroids from 3β-hydroxysteroids. Transgenic plants expressing cholesterol oxidase have been investigated in the fight against the cotton boll weevil. CHOD has also been used as a molecular probe to elucidate cellular membrane structures.
Cholesterol oxidase has been used in a study to improve treatment of oxysterol-mediated cytotoxicity. Cholesterol oxidase has also been used in a study that determined that cholesterol depletion impairs coupling between channel opening and vesicle release by allowing voltage-gated calcium channels to move further from release sites.

Biochem/physiol Actions

Cholesterol oxidase (CHOD) is a monomeric flavoprotein containing FAD that catalyzes the first step in cholesterol catabolism. This bifunctional enzyme oxidizes cholesterol to cholest-5-en-3-one in an FAD-requiring step. The pH optimum of the enzyme is 7.0 to 7.5 and temperature optimum is 50 °C. The pH stability is 5.7-7.8. Hg2+, Ag+, and ionic detergents inhibit the enzyme activity. Molecular mass of the enzyme is 62 kDa. Pathogenic bacteria require CHOD to infect a host′s macrophage.

Preparation Note

CHOD is soluble in cold 50 mM potassium phosphate buffer, pH 7.0. Prepare solutions immediately before use.

Other Notes

One unit will convert 1.0 μmole of cholesterol to 4-cholesten-3-one per min at pH 7.5 at 25 °C. Note: 4-cholesten-3-one may undergo isomerization.

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Cholesterol Oxidase from microorganisms aqueous solution, ≥30 units/mg protein (biuret)

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P4591

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description

aqueous solution, ≥30 units/mg protein (biuret)

description

lyophilized powder, ≥50 units/mg protein, recombinant, expressed in E. coli

description

recombinant, lyophilized powder, ≥10 units/mg protein

description

lyophilized powder, ≥1.5 U/mg

specific activity

≥30 units/mg protein (biuret)

specific activity

≥50 units/mg protein

specific activity

≥10 units/mg protein

specific activity

≥1.5 U/mg

form

aqueous solution

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

solubility

50 mM potassium phosphate buffer, pH 7.0: soluble (Cold)

solubility

-

solubility

50 mM potassium phosphate buffer, pH 7.0: soluble

solubility

-

mol wt

62 kDa

mol wt

64 kDa

mol wt

55 kDa

mol wt

~260 kDa

shipped in

dry ice

shipped in

-

shipped in

-

shipped in

-

storage temp.

−70°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

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Hazard Codes

H334

Pictograms

Health hazard
GHS08

Signal Word

Danger

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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