C5896
Choline Oxidase from Alcaligenes sp.
lyophilized powder, ≥10 units/mg solid
Synonym(s):
Choline: oxygen 1-oxidoreductase
Select a Size
| Size/SKU | Availability | Price |
|---|---|---|
50 units | Check Cart for Availability | $109.00 |
100 units | Check Cart for Availability | $200.00 |
500 units | Check Cart for Availability | $589.00 |
1000 units | Check Cart for Availability | $982.00 |
About This Item
form
lyophilized powder
Quality Segment
specific activity
≥10 units/mg solid
mol wt
~95 kDa
technique(s)
cell based assay: suitable
suitability
suitable for assay of choline, suitable for molecular biology
storage temp.
−20°C
General description
Michaelis constants:2.84 x 10¯3M (Choline), 5.33 x 10¯3M (Betaine aldehyde)
Structure:One mol of FAD is covalently bound to mol of the enzyme
Inhibitors:p-Chloromercuribenzoate, Cu++, Co++, Hg++, Ag+
Optimum pH:8.0 – 8.5
Optimum temp:40 – 45°C
pH Stability:pH 7.0 – 9.0 (30°C, 2hr)
Thermal stability:Below 37°C (pH 7.5, 10min)
Application
- in a study to investigate a choline biosensor constructed with a chitinous membrane and its application in measuring cholinesterase inhibitory activities
- has also been used in spectroscopic studies on the photoreaction of choline oxidase with covalently bound flavin.
- as a component of phosphate buffer saline (PBS) buffer for measurement of choline acetyltransferase (ChAT) activity in brain tissue homogenates
- to measure the choline generated by autotaxin (ATX) by ATX activity assay and phospholipase-D (PLD) by PLD activity assay
Biochem/physiol Actions
Other Notes
1 of 1
This Item | |||
|---|---|---|---|
| description lyophilized powder, ≥10 units/mg solid | description lyophilized powder, 8-20 units/mg solid | description lyophilized powder, ≥50 units/mg protein, recombinant, expressed in E. coli | description recombinant, lyophilized powder, ≥10 units/mg protein |
| specific activity ≥10 units/mg solid | specific activity 8-20 units/mg solid | specific activity ≥50 units/mg protein | specific activity ≥10 units/mg protein |
| technique(s) cell based assay: suitable | technique(s) - | technique(s) - | technique(s) - |
| suitability suitable for assay of choline, suitable for molecular biology | suitability - | suitability - | suitability - |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| mol wt ~95 kDa | mol wt 71 kDa by SDS-PAGE, 83 kDa by gel filtration | mol wt 64 kDa | mol wt 55 kDa |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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