Skip to Content
MilliporeSigma

Key Documents

View All Documentation

Skip To

C7104

Anti-Cdc27 antibody,Mouse monoclonal

clone AF3.1, purified from hybridoma cell culture

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View
Pack SizeSKUAvailabilityPrice
0.2 mL

Available to ship TODAYfromMILWAUKEE

$571.00
$485.35

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD01633250
Conjugate:
unconjugated
Clone:
AF3.1, monoclonal
Application:
ARR, ELISA (i), ICC, IHC (p), IP, WB
Citations:
15

$485.35

List Price$571.00Save 15%
Web-Only Promotion

Available to ship TODAYDetails

​
Request a Bulk Order
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

AF3.1, monoclonal

form

buffered aqueous solution

mol wt

antigen 97 kDa

species reactivity

mouse, Xenopus, rat, bovine, canine, human

concentration

~2 mg/mL

technique(s)

immunocytochemistry: suitable using 4% paraformaldehyde fixation, Triton X-100 permeabilization, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, microarray: suitable, western blot: 1-2 μg/mL using a HeLa Cell nuclear extract

isotype

IgG2b

UniProt accession no.

P30260

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CDC27(996)
mouse ... Cdc27(217232)
rat ... Cdc27(360643)

General description

Monoclonal Anti-Cdc27 (mouse IgG2b isotype) is derived from the AF3.1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a synthetic peptide corresponding to C-terminal of human Cdc27, conjugated to KLH.
The gene CDC27 (Cell division cycle protein 27 homolog) is mapped to human chromosome 17q12–23.2. The protein localizes in the nucleus.

Immunogen

synthetic peptide corresponding to the C-terminus 10 residues of human Cdc27.

Application

Monoclonal Anti-Cdc27 antibody produced in mouse is suitable for the following applications:
  • Immunocytochemistry using 4% paraformaldehyde fixation and Triton X-100 permeabilization
  • Immunohistochemistry (formalin-fixed, paraffin-embedded sections)
  • Immunoprecipitation.
  • Indirect ELISA
  • Microarray
  • Western blotting / immunoblotting at a concentration of 1-2μg/mL using a HeLa Cell nuclear extract.
  • Anaphase-promoting complex/cyclosome APC/C Purification.

Biochem/physiol Actions

CDC27 (Cell division cycle protein 27 homolog) is a component of anaphase-promoting complex/cyclosome (APC/C), an E3 ligase involved in cell cycle control. Phosphorylation of CDC27 is crucial for tumor growth factorβ (TGFβ)-induced activation of APC. It interacts with Microcephalin (MCPH1), causative gene for primary recessive autosomal microcephaly. DNA-damage response mediator MDC1 interacts with APC/C through CDC27. Down-regulation of CDC27 in irradiated cervical cancer cell line results in greater survival fraction. Polymorphism in CDC27 are linked with breast cancer.
The Cdc16/27/34 complex or the Anaphase-promoting complex/cyclosome (APC/C) complex functions as an ubiquitin conjugating enzyme, ubiquitinating cyclin B, and resulting in cyclin B/Cdk complex degradation.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
C7855M7815H4538
Anti-Cdc27 antibody,Mouse monoclonal clone AF3.1, purified from hybridoma cell culture

Sigma-Aldrich

C7104

Anti-Cdc27 antibody,Mouse monoclonal

Anti-Cdh1 antibody,Mouse monoclonal clone DCS-266, purified from hybridoma cell culture

Sigma-Aldrich

C7855

Anti-Cdh1 antibody,Mouse monoclonal

Anti-MDM2 antibody ,Mouse monoclonal clone HDM2-323, purified from hybridoma cell culture

Sigma-Aldrich

M7815

Anti-MDM2 antibody ,Mouse monoclonal

Anti-Histone Deacetylase 5 (HDAC5) antibody, Mouse monoclonal clone HDAC5-35, purified from hybridoma cell culture

Sigma-Aldrich

H4538

Anti-Histone Deacetylase 5 (HDAC5) antibody, Mouse monoclonal

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

species reactivity

mouse, Xenopus, rat, bovine, canine, human

species reactivity

human

species reactivity

human, mouse

species reactivity

rat, mouse, human

Gene Information

human ... CDC27(996)
mouse ... Cdc27(217232)
rat ... Cdc27(360643)

Gene Information

human ... CDH1(999)

Gene Information

human ... MDM2(4193)
mouse ... Mdm2(17246)

Gene Information

human ... HDAC5(10014)
mouse ... Hdac5(15184)
rat ... Hdac5(84580)

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

clone

AF3.1, monoclonal

clone

DCS-266, monoclonal

clone

HDM2-323, monoclonal

clone

HDAC5-35, monoclonal

Still not finding the right product?

Try our Product Selector Tool to narrow your options

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000184300
0000090046
0000184300
0000090046
037M4752

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Related Content

Sanjay Shete et al.
Cancer research, 71(24), 7568-7575 (2011-11-01)
Gliomas, which generally have a poor prognosis, are the most common primary malignant brain tumors in adults. Recent genome-wide association studies have shown that inherited susceptibility plays a role in the development of glioma. Although first-degree relatives of patients exhibit
A 20S complex containing CDC27 and CDC16 catalyzes the mitosis-specific conjugation of ubiquitin to cyclin B
King R W, e al.
Cell, 81(2), 279-288 (1995)
Hui Guo et al.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 36(7), 5299-5304 (2015-02-15)
Cdc27, as a core component of anaphase-promoting complex (APC), is a cell cycle regulator, which participates in control of mitotic checkpoint and surveys the mitotic spindle to maintain chromosomal integrity. It was hypothesized that polymorphisms in cdc27 gene might contribute
View All Related Papers

Global Trade Item Number

SKUGTIN
C7104-.2ML04061837867842

Questions

Reviews

No rating value

Active Filters