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Anti-CENP-E antibody produced in rabbit

Name: Anti-CENP-E antibody produced in rabbit
Brand: SIGMA

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Anti-Centromere-associated protein-E

About This Item

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD05665206

Conjugate:

unconjugated

Clone:

polyclonal

Application:

ARR, ICC, WB

Citations:

This product is discontinued. Contact Technical Servicefor assistance.

Properties

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~310 kDa

species reactivity

human

technique(s)

immunocytochemistry: suitable using human epitheloid carcinoma HeLa cell line., microarray: suitable, western blot: 1:2,000 using whole extract of HeLa nuclear cell line

UniProt accession no.

Q02224

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CENPE(1062)

Description

General description

Centromere-associated protein-E (CENP-E, 312 kDa) is a member of the kinesin superfamily of microtubule motor proteins and is an integral part of kinetochore corona fibers that link centromere to the spindle microtubules. CENP-E localizes to the kinetochore throughout all phases of mitotic chromosome movement from early prometaphase through anaphase A.

Immunogen

synthetic peptide corresponding to amino acids 2545-2563 located near the C-terminus of human CENP-E conjugated to KLH.

Application

Anti-CENP-E antibody produced in rabbit has been used in:

immunoblotting[1]
immunofluorescence[1]
immunocytochemistry.

Biochem/physiol Actions

Centromere-associated protein-E (CENP-E) plays an important role in attachment of kinetochores to spindle microtubules in the alignment of chromosomes and is an essential component of mitotic checkpoint signaling cascade. It functions as a motor in the initial chromosome movement at the mitotic midzone. CENP-E is associated with minus end-directed microtubule motor activity suggesting that CENP-E might be responsible for poleward kinetochore movements in prometaphase and anaphase A. Suppression of CENP-E synthesis by antisense CENP-E yields chromosomes that are chronically mono-oriented with flattened bipolar spindles and generates spindle poles fragments. Depletion of CENP-E leads to profound checkpoint activation and long-term mitotic arrest. CENP-E has been implicated as a binding partner for the mitotic checkpoint kinase BubR1 during mitosis.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	I5283	SAB1411421	ABE1957
$Anti-CENP-E antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich C7488 Anti-CENP-E antibody produced in rabbit	$Anti-INCENP antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich I5283 Anti-INCENP antibody produced in rabbit	Sigma-Aldrich SAB1411421 Anti-CENPM antibody produced in rabbit	Sigma-Aldrich ABE1957 Anti-CENP-C Antibody
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
antibody form IgG fraction of antiserum	antibody form IgG fraction of antiserum	antibody form purified immunoglobulin	antibody form serum
Quality Level 200	Quality Level 200	Quality Level -	Quality Level 100
biological source rabbit	biological source rabbit	biological source rabbit	biological source rabbit
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. -
form buffered aqueous solution	form buffered aqueous solution	form buffered aqueous solution	form -

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

057M4781V

063K4855

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Global Trade Item Number

SKU	GTIN
C7488-200UL	04061837793998

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