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Anti-Caspase 9 antibody produced in rabbit

Name: Anti-Caspase 9 antibody produced in rabbit
Brand: SIGMA
Price: 558 USD

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-APAF-3, Anti-APAF3, Anti-ICE-LAP6, Anti-MCH6, Anti-PPP1R56

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Pack Size	SKU	Availability	Price
0.2 mL		Check Cart for Availability	$558.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD01864176

Conjugate:

unconjugated

Clone:

polyclonal

Application:

ARR, IHC (p), IP, WB

Citations:

$558.00

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Properties

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 35-47 kDa (two bands)

species reactivity

human, rat

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40 using microwave-treated tissue sections of human and rat heart, immunoprecipitation (IP): 10 μg using HeLa mitochondrial RIPA lysate (500 μg), microarray: suitable, western blot: 1:300 using whole extract of human Jurkat cells

UniProt accession no.

P55211

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CASP9(842)
rat ... Casp9(58918)

Description

General description

Anti-Caspase 9 is developed in rabbit using a synthetic peptide corresponding to amino acid of human procaspase 9 with N-terminal added lysine conjugated to keyhole limpet hemocyanin (KLH) with glutaraldehyde. Caspase 9/CASP9 (Mch6, ICE-LAP6, APAF3) is a member of the caspase 2 subfamily and is classified together with caspases 8 and 10 as an initiator caspase. It is a cysteine containing aspartate specific protease, made up of 416 amino acids, of which 13 are cysteine. CASP9 is located on human chromosome 1p36.21.

Immunogen

synthetic peptide corresponding to amino acid residues 299-316 of human procaspase 9 with N-terminal added lysine conjugated to KLH with glutaraldehyde.

Application

Anti-Caspase 9 antibody produced in rabbit has been used in western blot analysis.[1]

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

Caspase 9/CASP9 is essential for apoptosis during normal development of the murine central nervous system and plays a role in controlling tumor development. It functions as an initiator caspase when mitochondrial dysfunction is the primary event in apoptosis. Caspase 9 functions as an initiator caspase when mitochondrial dysfunction is the primary event in apoptosis. Caspase 9 can also be activated by granzyme B.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	SAB4300683	SAB5700660	SAB5700197
Sigma-Aldrich C7729 Anti-Caspase 9 antibody produced in rabbit	Sigma-Aldrich SAB4300683 Anti-Caspase 9 antibody produced in rabbit	Sigma-Aldrich SAB5700660 Anti-Caspase 1 antibody produced in rabbit	Sigma-Aldrich SAB5700197 Anti-Caspase 8 antibody produced in rabbit
biological source rabbit	biological source rabbit	biological source rabbit	biological source rabbit
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
Quality Level 200	Quality Level 100	Quality Level 100	Quality Level 100
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody
UniProt accession no. P55211	UniProt accession no. -	UniProt accession no. P29466	UniProt accession no. Q14790
mol wt antigen 35-47 kDa (two bands)	mol wt ~47 kDa	mol wt 10, 29, 35, 42, 45	mol wt 25-32, 45- 61

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Global Trade Item Number

SKU	GTIN
C7729-.2ML	04061838104489

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