We have studied this product and determined it should be stored between 2-8 degrees C.
C7821
Curdlan
from Alcaligenes faecalis, ≥98% (TLC), solid
Synonym(s):
β-1,3-Glucan hydrate
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| Size/SKU | Availability | Price |
|---|---|---|
5 g | Check Cart for Availability | $457.00 |
About This Item
Form:
solid
General description
Curdlan is an insoluble natural polymer with linear structure mainly produced by Alcaligenes faecalis. It is a dietary fiber with gelatin properties.
Application
Curdlan from Alcaligenes faecalis has been used:
- for the stimulation of carp macrophages for regulation of differentially expressed genes
- to stimulate human intestinal epithelial cells for cytokine secretion
- to study its effect on transcriptional responses of inflammation/respiratory burst-associated genes in mouse alveolar macrophages by customized reverse transcription (RT)-PCR based arrays
- as a substrate in β-1,3–1,4-Glucanase activity assay
Biochem/physiol Actions
Curdlan is a (1,3)-β-glucan. It is a pathogen-associated molecular-pattern (PAMP) recognized by the receptor dectin-1. Activation of dectin-1, together with TLR2/TLR6, promotes signaling resulting in the production of pro-inflammatory cytokines, thus inducing inflammation.
Curdlan is used as a food additive in the food industry. It improves the elasticity and reduces the embrittling tendency of hydroxyapatite/chitosan scaffold, which aids in bone regeneration.
Preparation Note
For gelation at 70 °C, the concentration of the sodium hydroxide should be 0.01 M.
This product is tested for solubility in 0.1N sodium hydroxide at a concentration of 9.8 - 10.2 mg/mL. Heating and/or sonication are typically required for solubilization. This product is not sterile. For use in cell culture, stock solutions should be filter sterilized prior to use.
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This Item | |||
|---|---|---|---|
| description - | description ≥260 U/mL, aqueous solution | description Type XII-A, saline solution, ≥500 units/mg protein (biuret) | description lyophilized powder, ≥2,000 units/mg protein, Protein ≥20 % by biuret |
| form solid | form aqueous solution | form saline solution | form lyophilized powder |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 300 |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
Storage Class
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, type N95 (US)
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Why does the product needs to be stored at 4°C?
1 answer-
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How can I solubilize Curdlan (C7821) for use in cell stimulation?
1 answer-
This product is tested for solubility in 0.1N sodium hydroxide at a concentration of 9.8 - 10.2 mg/mL. Heating and/or sonication are typically required for solubilization. This product is not sterile. For use in cell culture, stock solutions should be filter sterilized prior to use. Please see link below to a sample Certificate of Analysis for more information:
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/C7821-5G0000231174.pdfHelpful?
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Do you test to confirm that product C7821, Curdlan, has (1,3)-β-glucan linkages?
1 answer-
We do not test to confirm that C7821, Curdlan, has (1,3)-β-glucan linkages on a lot to lot basis. However, we have verified product authenticity in the past. Our manufacturing procedure is reported in literature as yielding Curdlan. In addition, C13-NMR, chromatography, and IR analyses results compared to literature and commercial control product proved that product is a (1,3)-β glucan polymer.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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