The product is a buffered aqueous solution, not a lyophilized powder, so it does not require reconstitution. The solution contains Cathepsin B at a specific protein concentration and activity, both of which are lot-dependent and provided on the CoA.
C8571
Cathepsin B from human liver
buffered aqueous solution, ≥200 units/mg protein (One unit is defined as the amount of enzyme that hydrolyzes 1 μmol of Z-Arg-Arg-beta-naphthylamide per minute at 40 °C, using 100 mM Na/K phosphate, pH 6.0, with 1.33 mM EDTA and 2 mM DTT as the activation buffer.)
Synonym(s):
Cathepsin B1
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About This Item
CAS Number:
UNSPSC Code:
12352200
NACRES:
NA.32
MDL number:
Specific activity:
≥200 units/mg protein (One unit is defined as the amount of enzyme that hydrolyzes 1 μmol of Z-Arg-Arg-beta-naphthylamide per minute at 40 °C, using 100 mM Na/K phosphate, pH 6.0, with 1.33 mM EDTA and 2 mM DTT as the activation buffer.)
Biological source:
human liver
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biological source
human liver
form
buffered aqueous solution
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Quality Level
Gene Information
human ... CTSB(1508)
specific activity
≥200 units/mg protein (One unit is defined as the amount of enzyme that hydrolyzes 1 μmol of Z-Arg-Arg-beta-naphthylamide per minute at 40 °C, using 100 mM Na/K phosphate, pH 6.0, with 1.33 mM EDTA and 2 mM DTT as the activation buffer.)
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This Item | |||
|---|---|---|---|
| biological source human liver | biological source - | biological source human | biological source human liver |
| Gene Information human ... CTSB(1508) | Gene Information human ... CTSB(1508) | Gene Information human ... CTSB(1508) | Gene Information human ... CTSB(1508) |
| specific activity ≥1,500 units/mg protein (E1%/280) | specific activity ≥5 units/mg protein | specific activity ≥2228 pmol/min-μg | specific activity ≥10 units/mg protein |
| form buffered aqueous solution | form lyophilized powder | form aqueous solution | form liquid |
| shipped in dry ice | shipped in - | shipped in dry ice | shipped in wet ice |
| storage temp. −70°C | storage temp. −20°C | storage temp. −70°C | storage temp. −70°C |
General description
Cathepsin B gene is mapped to human chromosome 8p23.1.[1] It is a lysosomal cysteine with 252 amino acids distributed in two chains and is homologous to rat liver sequence.[2] It has disc shaped structure with two domains namely the left-hand ′L′ domain at the amino terminal and right hand ′R′ domain at the carboxyl-terminal.[3]
Application
Biochem/physiol Actions
Cathepsin B from human liver is highly expressed in the esophageal adenocarcinoma (EAC).[1] It reduces hepatic inflammation and fibrogenesis and may serve as a potential candidate for treating liver diseases.[7]
Cathepsin B has been found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells. Translocation of cathepsin B from the cytoplasm to the nucleus contributes to bile salt induced apoptosis of rat hepatocytes. Levels of cathepsin B in PC12 cells significantly decrease 12 to 24 hours after apoptosis is induced.
Found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells.
Packaging
Package size based on protein content
Physical form
Solution in 50 mM sodium acetate, pH 5.0, with 1 mM EDTA.
Other Notes
One unit will liberate 1 nanomole of 7-amino-4-methylcoumarin from Z-Arg-Arg 7-amido-4-methylcoumarin per min at pH 6.0 at 40 °C.
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Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Cathepsin B inactivation attenuates hepatic injury and fibrosis during cholestasis
Canbay A, et al.
The Journal of Clinical Investigation, 112(2), 152-159 (2003)
Genome-wide catalogue of chromosomal aberrations in barrett's esophagus and esophageal adenocarcinoma: a high-density single nucleotide polymorphism array analysis
Gu J, et al.
Cancer Prevention Research (Philadelphia, Pa.), 3(9), 1176-1186 (2010)
The refined 2.15 AX-ray crystal structure of human liver cathepsin B: the structural basis for its specificity.
Musil DJ, et al.
The Embo Journal, 10(9), 2321-2330 (1991)
Prevention of free fatty acid-induced hepatic lipotoxicity by 18beta-glycyrrhetinic acid through lysosomal and mitochondrial pathways
Wu X, et al.
Hepatology, 47(6), 1905-1915 (2008)
Amino acid sequence of human liver cathepsin B
Ritonja A, et al.
Febs Letters, 181(1), 169-172 (1985)
Articles
Cathepsin B is a lysosomal cysteine proteinase with broad specificity. This protocol uses Nα–CBZ–Arg–Arg–7–amido–4–methylcoumarin as the substrate for fluorometric detection of Cathepsin B activity.
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how to reconstitute the cathepsin B? If it is written >1500units/mg, and C8571-25UG, how come it is in aqeous solution?
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