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Anti-Chloramphenicol Acetyl Transferase (CAT) antibody produced in rabbit

Name: Anti-Chloramphenicol Acetyl Transferase (CAT) antibody produced in rabbit
Brand: SIGMA
Price: 506.92 USD
Availability: InStock

IgG fraction of antiserum, buffered aqueous solution

Enhanced Validation

Synonym(s):

CAT Antibody - Anti-Chloramphenicol Acetyl Transferase (CAT) antibody produced in rabbit, Cat Antibody

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Size/SKU	Availability	Price
0.5 mL	Check Cart for Availability	$551.00 $506.92

About This Item

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD01092314

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IF, WB

Citations:

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Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 26 kDa

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

indirect immunofluorescence: 10 μg/mL using eukaryotic cells transfected with a plasmid bearing the CAT gene, western blot: 10 μg/mL using eukaryotic cells transfected with a plasmid bearing the CAT gene

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

Chloramphenicol Acetyl Transferases (CATs) shows conservation and differences in their amino acid sequences. CAT is encode by cat gene and exists as monomer and later assemble into a trimer.

Immunogen

bacterial chloramphenicol acetyl transferase (CAT).

Application

Anti-Chloramphenicol Acetyl Transferase (CAT) has been used in

immunoblotting
indirect immunofluorescence
immunofluorescence microscopy

Biochem/physiol Actions

Anti-Chloramphenicol Acetyl Transferase (CAT) antibody is specific for bacterial CAT and recombinant CAT expressed in transfected eukaryotic cells (a predominant band of approx. 26 kD).

Bacterial chloramphenicol acetyl transferase (CAT) is an enzyme that catalyzes the inactivation of the antibiotic, chloramphenicol, by acetylation and subsequently confers bacterial resistance to the antibiotic. CAT, being a stable prokaryotic enzyme, is often used as a reporter gene in transfection assays developed for eukaryotic promoters. Quantification of reporter gene expressions, such as that of CAT, can be correlated to the transcriptional functions of the target sequence. Thus, antibodies directed against CAT can be used for the study of gene sequences that are fused to the CAT reporter gene
Anti-Chloramphenicol Acetyl Transferase (CAT) antibody is specific for bacterial CAT and recombinant CAT expressed in transfected eukaryotic cells (a predominant band of approx. 26 kD). Staining of CAT by the antibody is inhibited by the bacterial CAT antigen in cells transfected with CAT.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	MAB3678	SAB3700078	SAB3700068
$Anti-Chloramphenicol Acetyl Transferase (CAT) antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich C9336 Anti-Chloramphenicol Acetyl Transferase (CAT) antibody produced in rabbit	Sigma-Aldrich MAB3678 Anti-Chloramphenicol Acetyltransferase Antibody, clone CAT-1	Sigma-Aldrich SAB3700078 Anti-Cat IgG (H+L) antibody produced in rabbit	Sigma-Aldrich SAB3700068 Anti-Cat IgG (Fc specific)-Biotin antibody produced in rabbit
description IgG fraction of antiserum, buffered aqueous solution	description ascites fluid, clone CAT-1, Chemicon^®	description affinity isolated antibody, buffered aqueous solution	description affinity isolated antibody, lyophilized powder
biological source rabbit	biological source mouse	biological source rabbit	biological source rabbit
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate biotin conjugate
Quality Level 200	Quality Level 100	Quality Level 100	Quality Level 100
antibody form IgG fraction of antiserum	antibody form ascites fluid	antibody form affinity isolated antibody	antibody form affinity isolated antibody
storage temp. −20°C	storage temp. -	storage temp. 2-8°C	storage temp. 2-8°C
technique(s) western blot: 10 μg/mL using eukaryotic cells transfected with a plasmid bearing the CAT gene, indirect immunofluorescence: 10 μg/mL using eukaryotic cells transfected with a plasmid bearing the CAT gene	technique(s) immunocytochemistry: suitable, western blot: suitable	technique(s) indirect ELISA: suitable, western blot: suitable, immunohistochemistry: suitable	technique(s) western blot: suitable, immunohistochemistry: suitable, indirect ELISA: suitable

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Storage Class

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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