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C9972

Cholera Toxin B subunit

mucosal immunogen, lyophilized powder

Synonym(s):

CTB

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Pack SizeSKUAvailabilityPrice
0.5 mg

Available to ship TODAYfromMILWAUKEE

$308.00

About This Item

UNSPSC Code:
12352200
PubChem Substance ID:
329775297
NACRES:
NA.77
MDL number:
MFCD02356522
Form:
lyophilized powder
Quality level:
200

$308.00

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Product Name

Cholera Toxin B subunit, biotin conjugate, lyophilized powder

conjugate

biotin conjugate

Quality Level

200

form

lyophilized powder

mol wt

~12 kDa

composition

Protein, ~40% Lowry

storage temp.

2-8°C

SMILES string

CCOc1ccccc1C(=O)Nc2ccc(Cl)c(c2)C(F)(F)F

InChI

1S/C16H13ClF3NO2/c1-2-23-14-6-4-3-5-11(14)15(22)21-10-7-8-13(17)12(9-10)16(18,19)20/h3-9H,2H2,1H3,(H,21,22)

InChI key

YDXZSNHARVUYNM-UHFFFAOYSA-N

General description

Cholera Toxin B (CTB) is secreted by Vibrio cholerae. CTB functions as an oral subunit vaccine for cholera, which is associated with acute watery diarrhoea. It acts as a mucosal immunogen. Cholera toxin (CT) stimulates cell surface molecules, such as antigen presenting cells (APCs), murine and human dendritic cells (DCs). CT also has immunomodulatory properties. It induces the secretion of interleukin 1 (IL-1) from macrophages and enhances their APC function.

Application

Cholera Toxin B subunit has been used:
  • in immunofluorescence[1]
  • in the analysis of major histocompatibility complex (MHC) class II lipid raft partitioning
  • in live cell three-dimensional tracking of SH-SY5Y human neuroblastoma cells
  • to assess the toll-like receptors (TLR) and FcRγ (Fc receptor γ chain) – CARD9 (caspase recruitment domain family member 9) activation by cholera Toxin B (CTB)

Biochem/physiol Actions

The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport.
The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic.

Physical form

Lyophilized powder containing sodium phosphate buffer salts, sodium azide and sodium EDTA.

Analysis Note

Activity measured by ELISA using ganglioside GM1-coated multiwell plates, rabbit anti-Cholera toxin B subunit, and peroxidase-labeled goat anti-rabbit IgG as the secondary antibody. 50% saturation of binding is achieved with 0.02-1 μg of Cholera toxin B subunit-biotin conjugate per mL. The conjugated B subunit gives a similar value for 50% binding to that of unconjugated B subunit from which it is prepared.
Biotin content ~1.0 mole/mole protein.

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Sigma-Aldrich

C3741

Cholera Toxin B subunit

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

200

mol wt

~12 kDa

mol wt

~12 kDa

mol wt

~12 kDa

mol wt

pentamer 57 kDa, ~12 kDa

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

conjugate

biotin conjugate

conjugate

FITC conjugate

conjugate

-

conjugate

peroxidase conjugate (Contains ~ 2 moles HRP/mole of CTB. ~100 μg HRP conjugated to ~45 μg CTB)

composition

Protein, ~40% Lowry

composition

Protein, ~20% Lowry

composition

Protein, ~5% Lowry

composition

-

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hcodes

H412

Hazard Classifications

Aquatic Chronic 3

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000454816
0000454816
0000359172
0000359172
0000299635

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Related Content

Data Sheet - C9972

Instructions

Evidence for TLR4 and FcRgamma-CARD9 activation by cholera toxin B subunit and its direct bindings to TREM2 and LMIR5 receptors
Phongsisay V, et al.
Molecular Immunology, 66(2), 463-471 (2015)
The Ia. 2 epitope defines a subset of lipid raft-resident MHC class II molecules crucial to effective antigen presentation
Busman-Sahay K, et al.
Journal of Immunology, 1100336-1100336 (2011)
Lucia Gardini et al.
Scientific reports, 5, 16088-16088 (2015-11-04)
Live cells are three-dimensional environments where biological molecules move to find their targets and accomplish their functions. However, up to now, most single molecule investigations have been limited to bi-dimensional studies owing to the complexity of 3d-tracking techniques. Here, we
View All Related Papers

Global Trade Item Number

SKUGTIN
C9972-.5MG04061832981093

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