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CAT100

Catalase Assay Kit

sufficient for ≥100 tests enzymatic, determination of catalase activity in tissues and cells

Sinonimo/i:

Catalase Activity Detection Kit

per visualizzare i prezzi organizzativi e contrattuali.

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A voi/SKUDisponibilitàPrezzo
1 kit
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
1510,00 €

Informazioni su questo articolo

NACRES:
NA.84
UNSPSC Code:
12161503

1510,00 €

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Quality Segment

200

usage

sufficient for ≥100 tests enzymatic

detection method

colorimetric

shipped in

wet ice

storage temp.

2-8°C

Gene Information

human ... CAT(847)

General description

The Catalase Assay Kit contains all necessary components for studying catalase activity in various tissues and subcellular organelles.

Application

Suitable for studying catalase activity in various tissues and subcellular organelles.
Sutitable for Colorimetric and UV Assays

Biochem/physiol Actions

Catalase is a ubiquitous antioxidant enzyme which catalyses the decomposition of hydrogen peroxide (H2O2) to water and oxygen. Hydrogen peroxide is formed in the eukaryotic cell as a by-product of various oxidases and superoxide dismutases. Hydrogen peroxide accumulation in cells causes oxidation of cellular targets such as DNA, proteins, and lipids leading to mutagenesis and cell death. Removal of the H2O2 from the cell by catalase provides protection against oxidative damage to living cells and its role in oxidative stress related diseases has been widely studied.
This assay method is based on the measurement of the hydrogen peroxide substrate remaining after the action of catalase. First, the catalase converts hydrogen peroxide to water and oxygen (catalatic pathway) and then this enzymatic reaction is stopped with sodium azide. An aliquot of the reaction mix is then assayed for the amount of hydrogen peroxide remaining by a colorimetric method.10 The colorimetric method uses a substituted phenol (3,5-dichloro-2-hydroxybenzenesulfonic acid), which couples oxidatively to 4-aminoantipyrine in the presence of hydrogen peroxide and horseradish peroxidase (HRP) to give a red quinoneimine dye (N-(4-antipyryl)-3-chloro-5-sulfonatep-benzoquinone-monoimine) that absorbs at 520 nm

Features and Benefits

  • Useful for determining catalase activity - may be used in various tissues and cells
  • Simple, optimized protocol - A simple colorimetric assay for analysis of peroxisome enrichment and catalase activity

Preparation Note

Use ultrapure water in preparation of all solutions.

Other Notes

One unit of catalase will decompose 1.0 micromole of hydrogen peroxide to oxygen and water per minute at pH 7.0 at 25 °C at a substrate concentration of 50 mM hydrogen peroxide.

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CGP1CS0260C3556
Catalase Assay Kit sufficient for ≥100 tests enzymatic, determination of catalase activity in tissues and cells

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Sigma-Aldrich

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Catalase from human erythrocytes ≥90% (SDS-PAGE), buffered aqueous solution, ≥30,000 units/mg protein

Sigma-Aldrich

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Catalase from human erythrocytes

Gene Information

human ... CAT(847)

Gene Information

-

Gene Information

human ... GSR(2936)

Gene Information

human ... CAT(847)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

usage

sufficient for ≥100 tests enzymatic

usage

sufficient for 100 tests

usage

sufficient for 700 assays

usage

-

detection method

colorimetric

detection method

colorimetric

detection method

-

detection method

-

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

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SDS & Determinazione del prezzo
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    Peroxidase from horseradish, Type VI-A, essentially salt-free, lyophilized powder, 950-2000 units/mg solid (using ABTS), ≥250 units/mg solid (using pyrogallol) 5 mg
    SDS
  • 323381
    Hydrogen peroxide solution, contains ~200 ppm acetanilide as stabilizer, 3 wt. % in H2O
    SDS

signalword

Danger

Classe di stoccaggio

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2

Elenchi normativi

Forniamo informazioni su eventuali restrizioni prevalentemente per i prodotti chimici. Per altre tipologie di prodotto siamo in grado di fornire soltanto informazioni limitate. Nessuna segnalazione significa che nessuno dei componenti è citato in un elenco. È dovere dell’utilizzatore assicurarsi che il prodotto venga impiegato in maniera sicura e a norme di legge.

7783-20-2

CAS No.

CAS No.

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Certificati d'analisi (COA)

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  1. How can I determine the shelf life / expiration / retest date of this product?

    1 risposta

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 risposta

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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