CRISPRICON
Human CRISPRi Control and Effector Kit
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| Size/SKU | Availability | Price |
|---|---|---|
1 kit | Check Cart for Availability | $2,260.00 |
About This Item
NACRES:
NE.02
UNSPSC Code:
12352200
packaging
pkg of 12 vials (4x50μL aliquots for each of the 3 kit components)
application(s)
CRISPR
CRISPR
shipped in
dry ice
storage temp.
−70°C
General description
The control kit includes validated positive control targeting RAB1A and non-targeting control for assay set-up and a UCOE KRAB-dCas9 for consistent effector expression across a wide variety of cell lines. The kit is ideal for setting up the screen assay and optimization before using the CRISPRi library pools.
Application
- Set up and optimization of screen assay
- Functional Genomics/Target Validation
- Creation of cell lines stably expressing KRAB-dCas9
- Validated Positive and negative controls
Biochem/physiol Actions
The power of CRISPR for genome engineering, coupled with the ability to perform large-scale, whole genome, loss-of-function (LOF) screening, has allowed for new breakthroughs identifying gene pathways in drug resistance and disease. CRISPR is most commonly used to create double-stranded breaks that often result in loss of gene function (CRISPR-KO). However, the full extent of CRISPR′s utility extends beyond just targeted cutting of DNA. Nuclease-independent applications of CRISPR provide equal targeting specificity but instead of cutting, CRISPRi allows for targeted interference of gene function by delivering transcriptional repressor domains to a specific target sequence using modified dCas9 + gRNA complexes. Gene knockdown is complementary to CRISPR-KO and CRISPRa (activation) and has distinct advantages over existing loss-of-function strategies like RNAi. We partnered with University of California San Francisco to provide the best-in-class CRISPRi screening tools available.
Features and Benefits
- Ease of optimization: Validated positive control targeting RAB1A and non-targeting control for assay set up
- Best in Class UCSF gRNA selection algorithm and optimized (F+E) gRNA scaffold
- UCOE KRAB-dCas9 for consistent effector expression across a wide variety of cell lines
- Libraries provided at high functional titer, based on FACs or CFU analysis
- Use puromycin and or BFP as selection markers
Other Notes
2 Controls and 1 Effector Construct with a minimum concentration of 5x105 TU/mL(via FACS or CFU assay)
CRISPRIE: KRAB-dCas9 - Blasticidin CRISPRi Construct Lentiviral Transduction Particles
CRISPRI01: CRISPRi RAB1A Puromycin Control Transduction Particles
CRISPRI06: CRISPRi Nontargeting Control Puromycin Transduction Particles
For more information about screening with CRISPRi/a please Click Here.
CRISPRIE: KRAB-dCas9 - Blasticidin CRISPRi Construct Lentiviral Transduction Particles
CRISPRI01: CRISPRi RAB1A Puromycin Control Transduction Particles
CRISPRI06: CRISPRi Nontargeting Control Puromycin Transduction Particles
For more information about screening with CRISPRi/a please Click Here.
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This Item | |||
|---|---|---|---|
| description - | description - | description - | description - |
| application(s) CRISPR | application(s) CRISPR | application(s) CRISPR | application(s) CRISPR |
| storage temp. −70°C | storage temp. −70°C | storage temp. −70°C | storage temp. −70°C |
| packaging pkg of 12 vials (4x50μL aliquots for each of the 3 kit components) | packaging pkg of 20 vials (4x50μL aliquots for each of the 5 kit components) | packaging pkg of 20 vials (4x50μL aliquots for each of the 5 kit components) | packaging pkg of 20 vials (4x50μL aliquots for each of the 5 kit components) |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
Storage Class
12 - Non Combustible Liquids
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