CS1030
Chitinase Assay Kit, Fluorimetric
sufficient for 200 multiwell tests
Synonym(s):
Chitinase Detection Kit
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| Size/SKU | Availability | Price |
|---|---|---|
1 kit | Estimated to ship TODAYfromSAINT LOUIS | $995.00 |
About This Item
NACRES:
NA.84
UNSPSC Code:
12161503
usage
sufficient for 200 multiwell tests
Quality Segment
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... CHIT1(1118)
General description
The kit assay is based on the enzymatic hydrolysis of chitinase substrates. This enzymatic hydrolysis releases 4-methylumbelliferone (4MU), which upon ionization in basic pH, can be measured fluorimetrically at an excitation wavelength of 360 nm and an emission wavelength of 450 nm. The use of fluorimetric substrates provides a very sensitive detection system.
Application
The kit was tested and found suitable for Trichoderma viride and Streptomyces griseus, along with Hela, Jurkat, CHO, NIH-3T3, U-837 mammalian cell lines, human macropages, rat lung, kidney, liver, and brain tissue.
The Chitinase Assay Kit provides all the reagents required for efficient and sensitive detection of chitinase activity in fungal and bacterial growth media, macrophage lysates, and purified enzyme preparations. In addition, the kit provides three different substrates for the detection of the various types of the chitinolytic activity:
- 4-Methylumbelliferyl N,N′-diacetyl-β-D-chitobioside - substrate suitable for exochitinase activity detection (chitobiosidase activity)
- 4-Methylumbelliferyl N-acetyl-β-D-glucosaminide - substrate suitable for exochitinase activity detection (β-N-acetylglucosaminidase activity)
- 4-Methylumbelliferyl β-D-N,N′,N′′-triacetylchitotriose - substrate suitable for endochitinase activity detection
Biochem/physiol Actions
Chitinase catalyzes the hydrolytic cleavage of the β-1→4-glycoside bond present in biopolymers of N-acetylglucosamine, primarily in chitin. Chitinases are widely distributed in living organisms and are found in fungi, bacteria, parasites, plants, and animals. They are classified in families based on amino acid sequence similarities.
The chitinolytic enzymes are also categorized based on their enzymatic action on chitin substrates. Endochitinases are defined as the enzymes catalyzing the random cleavage at internal points in the chitin chain. Exochitinases catalyze the progressive release of acetylchitobiose or N-acetylglucosamine from the non-reducing end of chitin, and are referred to as chitobiosidase and β-N-acetylglucosaminidase, respectively.
Chitinases perform different functions in different organisms. In bacteria, they are mainly involved in nutritional processes. In yeast and various fungi, these enzymes participate in morphogenesis. In animals and plants, chitinases primarily play a role in the defense of the organism against pathogen attack.
The chitinolytic enzymes are also categorized based on their enzymatic action on chitin substrates. Endochitinases are defined as the enzymes catalyzing the random cleavage at internal points in the chitin chain. Exochitinases catalyze the progressive release of acetylchitobiose or N-acetylglucosamine from the non-reducing end of chitin, and are referred to as chitobiosidase and β-N-acetylglucosaminidase, respectively.
Chitinases perform different functions in different organisms. In bacteria, they are mainly involved in nutritional processes. In yeast and various fungi, these enzymes participate in morphogenesis. In animals and plants, chitinases primarily play a role in the defense of the organism against pathogen attack.
Preparation Note
Use ultrapure water for preparation of reagents.
1 of 1
This Item | |||
|---|---|---|---|
| description sufficient for 200 multiwell tests | description sufficient for 100 multiwell tests | description sufficient for 200 multiwell tests | description - |
| Gene Information human ... CHIT1(1118) | Gene Information human ... CHIA(27159), CHIT1(1118) | Gene Information human ... GSTA4(2941) | Gene Information human ... CASP3(836) |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| usage sufficient for 200 multiwell tests | usage sufficient for 100 multiwell tests | usage sufficient for 200 multiwell tests | usage sufficient for 1,000 multiwell tests, sufficient for 100 standard tests (2 ml) |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in dry ice |
Kit Components Only
Product No.
Description
- Assay Buffer 25 mL
- 4-Methylumbelliferyl N-acetyl-β-D-glucosaminide 5 mg
- 4-Methylumbelliferyl β-D-N,N′-diacetylchitobioside hydrate 5 mg
- 4-Methylumbelliferyl β-D-N,N′,N′′-triacetylchitotriose 5 mg
- Chitinase from Trichoderma viride 1 mg
- 4-Methylumbelliferone Standard Solution, 50 mg/mL 1 mL
- Sodium Carbonate 2 g
See All (8)
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
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