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Anti-phospho-DARPP32 (pThr⁷⁵) antibody produced in rabbit

Name: Anti-phospho-DARPP32 (pThr<SUP>75</SUP>) antibody produced in rabbit
Brand: SIGMA
Price: 768 USD
Availability: InStock

affinity isolated antibody, buffered aqueous glycerol solution, sufficient for 10 blots

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1 vial	Check Cart for Availability	$768.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD08705496

Conjugate:

unconjugated

Clone:

polyclonal

Application:

DB, WB

Citations:

$768.00

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Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

usage

sufficient for 10 blots

mol wt

antigen 32 kDa

species reactivity

mouse, human

technique(s)

dot blot: 1:1,000, western blot: 1:1,000

UniProt accession no.

Q9UD71

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pThr75)

Gene Information

human ... PPP1R1B(84152)
mouse ... Ppp1r1b(19049)

Description

General description

DARPP32 is a phosphoprotein with a molecular mass of 32kDa. It is regulated by dopamine (DA) and cAMP and is associated with dopaminoceptive neurons bearing D-1 receptors in the basal ganglia. DARPP32 is a target for the actions of dopamine.

DARPP32 phosphorylated on Thr⁷⁵ acts as an inhibitor of PKA.

Immunogen

synthetic phosphopeptide corresponding to amino acids residues surrounding the DARPP32 phosphorylated on threonine 75.

Application

Anti-phospho-DARPP32 (pThr75) antibody produced in rabbit is suitable for dot blot and western blot analysis at a working dilution of 1:1000 using rat caudate lysates.

Biochem/physiol Actions

Dopamine D1 receptor stimulation enhances cAMP formation, resulting in the phosphorylation of DARPP32. Phosphorylation of DARPP32 plays an important role in the regulation of dopaminergic neurotransmission. The activity of this protein has an important role in the actions of alcohol, caffeine and Prozac^®.

Physical form

Solution at 100 μL in 10 mM HEPES, pH 7.5, 150 mM NaCl, 100 μg/mL BSA, and 50% glycerol.

Other Notes

The labeling by the antibody to DARPP32 Thr⁷⁵ is markedly increased by okadaic acid treatment.

Legal Information

Prozac is a registered trademark of Eli Lilly and Co.

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This Item	SAB4503330	SAB4503329	HPA048630
Sigma-Aldrich D2693 Anti-phospho-DARPP32 (pThr⁷⁵) antibody produced in rabbit	Sigma-Aldrich SAB4503330 Anti-DARPP-32, N-Terminal antibody produced in rabbit	Sigma-Aldrich SAB4503329 Anti-DARPP-32 antibody produced in rabbit	Sigma-Aldrich HPA048630 Anti-PPP1R1B antibody produced in rabbit
description affinity isolated antibody, buffered aqueous glycerol solution, sufficient for 10 blots	description affinity isolated antibody	description affinity isolated antibody	description Prestige Antibodies^® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
biological source rabbit	biological source rabbit	biological source rabbit	biological source rabbit
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody
Quality Level 200	Quality Level 100	Quality Level 100	Quality Level 100
form buffered aqueous glycerol solution	form buffered aqueous solution	form buffered aqueous solution	form buffered aqueous glycerol solution
Gene Information human ... PPP1R1B(84152) mouse ... Ppp1r1b(19049)	Gene Information human ... PPP1R1B(84152)	Gene Information human ... PPP1R1B(84152)	Gene Information human ... PPP1R1B(84152)

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Storage Class

10 - Combustible liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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