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D8156

Monoclonal Anti-Digoxin antibody produced in mouse

clone DI-22, ascites fluid

Synonym(s):

Monoclonal Anti-Digoxin

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0.1 mL
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$568.00

About This Item

NACRES:
NA.43
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
DI-22, monoclonal
Application:
DB, ELISA (i), FACS
Citations:
31

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biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

DI-22, monoclonal

contains

15 mM sodium azide

technique(s)

dot blot: suitable, flow cytometry: suitable, indirect ELISA: 1:10,000 using digoxin-BSA, indirect ELISA: 1:2,500 using digoxigenin-transferrin

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Monoclonal Anti-Digoxin (mouse IgG1 isotype) is derived from the hybridoma DI-22 produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with digoxin- keyhole limpet hemocyanin (KLH). Digoxin is obatained from the leaves of the Digitalis lanata, a foxglove plant. The digoxin molecule is made up of a sugar and a cardenolide. It has a molecular weight is 780.95 Da. It is a odorless white crystal, which is soluble in alcohol and freely soluble in pyridine. It is expressed in the duodenum, kidneys, liver and the blood-brain barrier.

Immunogen

Digoxin-KLH.

Application

Monoclonal Anti-Digoxin antibody produced in mouse has been used in:
  • enzyme-linked immunosorbent assay (ELISA)
  • dot blot
  • flow cytometry
  • fluorescence in situ hybridization (FISH)
  • DNA hybridization
  • in-situ hybridization (ISH)
  • immunodetection of the probe and chromosome X

Biochem/physiol Actions

Antibody is specific for digoxin and shows a high affinity for digoxigenin.
Digoxin is a phytoestrogen that binds estrogen receptors and produces inotropic effects in cardiac muscle. Studies show that digoxin decreases the risk of breast and uterus cancers that are estrogen sensitive.
Digoxin is a substrate for P-glycoprotein (P-gp), a membrane efflux transporter. This antibody may be used to detect digoxin-labeled compounds such as oligonucleotides, antibodies or peptides.
Monoclonal Anti-Digoxin antibody is specific for digoxin and digoxin-labeled compounds, and shows strong cross-reactivity with digoxigenin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Monoclonal Anti-Digoxin antibody produced in mouse clone DI-22, ascites fluid

Sigma-Aldrich

D8156

Monoclonal Anti-Digoxin antibody produced in mouse

Anti-Digoxin antibody, Mouse monoclonal clone DI-22, purified from hybridoma cell culture

Sigma-Aldrich

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Anti-Digoxin antibody, Mouse monoclonal

Anti-Digoxin–Alkaline Phosphatase antibody, Mouse monoclonal clone DI-22, purified from hybridoma cell culture

Sigma-Aldrich

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Monoclonal Anti-β-Galactosidase antibody produced in mouse clone GAL-13, ascites fluid

Sigma-Aldrich

G8021

Monoclonal Anti-β-Galactosidase antibody produced in mouse

description

clone DI-22, ascites fluid

description

clone DI-22, purified from hybridoma cell culture

description

clone DI-22, purified from hybridoma cell culture

description

clone GAL-13, ascites fluid

antibody form

ascites fluid

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

antibody form

ascites fluid

conjugate

unconjugated

conjugate

-

conjugate

alkaline phosphatase conjugate

conjugate

unconjugated

clone

DI-22, monoclonal

clone

DI-22, monoclonal

clone

DI-22, monoclonal

clone

GAL-13, monoclonal

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

isotype

IgG1

isotype

IgG1

isotype

IgG1

isotype

IgG1

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

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12 - Non Combustible Liquids

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flash_point_f

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flash_point_c

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