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D8168

Anti-Dystrophin Antibody

mouse monoclonal, MANDYS8

Synonym(s):

Dystrophin Antibody, Dystrophin Antibody - Monoclonal Anti-Dystrophin antibody produced in mouse

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Size/SKUAvailabilityPrice
100 μL
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$397.00
0.2 mL
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$650.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD00164586
Conjugate:
unconjugated
Clone:
MANDYS8, monoclonal
Application:
ARR, ELISA (i), IF, WB
Citations:
71

$397.00

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Product Name

Monoclonal Anti-Dystrophin antibody produced in mouse, clone MANDYS8, ascites fluid

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

MANDYS8, monoclonal

species reactivity

chicken, rat, human, pig, rabbit, mouse

technique(s)

indirect ELISA: suitable, indirect immunofluorescence: 1:400 using frozen human or animal muscle tissue sections., microarray: suitable, western blot: suitable

isotype

IgG2b

UniProt accession no.

P11532

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... DMD(1756)
mouse ... Dmd(13405)
rat ... Dmd(24907)

General description

Monoclonal Anti-Dystrophin (mouse IgG2b isotype) is derived from the MANDYS8 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Dystrophin is a structural protein on the inner face of the membrane, consisting of a 25-repeat, rod-like, triple-helical domain separating an N-terminal actin binding domain from two C-terminal domains, one of which is rich in cysteine.
The rod domain of the human dystrophin molecule is present in normal muscle tissue and in nearly all Becker muscular dystrophies. It is absent in the cases of Duchenne muscular dystrophies and in the dystrophic mouse (mdx).

Immunogen

recombinant human dystrophin fragment.

Application

Monoclonal Anti-Dystrophin antibody produced in mouse has been used in
  • immunohistochemistry
  • immunofluorescence
  • double immunofluorescence terminal dUTP nick-end labeling (TUNEL)
  • immunoblotting

Monoclonal anti-dystrophin antibody can be used for localization of dystrophin using immunochemical assays like ELISA and immunohistochemistry. The antibody can also be used in immunoblotting for brain dystrophin. Further, it can be used in western blotting and double immunofluorescent labelling (diluted 1: 500) of dystrophin.

Biochem/physiol Actions

Dystrophin deficiency is associated with severe Duchenne muscular dystrophy (DMD). Becker muscular dystrophy (BMD) show less pronounced abnormalities of dystrophin protein expression. Since abnormalities in the protein expression occur specifically in patients with these types of muscular dystrophy, dystrophin analysis may be used to distinguish these conditions from other neuromuscular diseases.

Other Notes

This product can be found as purified product that was produced using cell culture hybridoma product.
SAB4200764 Anti-Dystrophin antibody, Mouse monoclonal
clone MANDYS8, purified from hybridoma cell culture

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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