This product can be reconstituted in water at 01. mg/mL. The solubility in PBS has not been determined, but is expected to be similar. Please see the link below to review additional preparation instructions included in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/272/751/d8935dat.pdf
D8935
Nidogen-1 (NID1) human
recombinant, expressed in HEK 293 cells, suitable for cell culture
Synonym(s):
Entactin, NID, NID-1, NID1
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 50 μg | Please contact Customer Service for Availability | $477.00 |
About This Item
NACRES:
NA.77
UNSPSC Code:
12352202
Form:
lyophilized powder
Assay:
95% (SDS-PAGE)
Recombinant:
expressed in HEK 293 cells
recombinant
expressed in HEK 293 cells
assay
95% (SDS-PAGE)
form
lyophilized powder
technique(s)
cell culture | mammalian: suitable
storage temp.
−20°C
1 of 1
This Item | |||
|---|---|---|---|
| assay 95% (SDS-PAGE) | assay ≥95% (SDS-PAGE) | assay ≥97% (HPLC), ≥97% (SDS-PAGE) | assay ≥95% (HPLC), ≥95% (SDS-PAGE) |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| recombinant expressed in HEK 293 cells | recombinant expressed in HEK 293 cells | recombinant expressed in HEK 293 cells | recombinant expressed in HEK 293 cells |
| form lyophilized powder | form lyophilized powder | form lyophilized | form lyophilized |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
General description
Nidogen-1 belongs to the nidogens family of glycoproteins. This family consists of nidogen-1 and nidogen-2, both of which are sulfated monomeric glycoproteins. Nidogen-1 is ubiquitously present in the synaptic and extra synaptic basal lamina. It is mapped to human chromosome 1q42.3. Nidogen-1 encodes a 139 kDa, three globular domains namely G1, G2 and G3 and has multiple epidermal growth factor repeats. Recombinant human nidogen-1 is expressed in human HEK 293 cells as a glycoprotein with a calculated molecular mass of 133.5 kDa (amino acids 29-1247). This protein is manufactured in human cells, with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports higher specific activity of the protein. The protein is produced with no artificial tags.
Application
This recombinant human nidogen-1 product can be used as an attachment factor for cells adherence.
Biochem/physiol Actions
Nidogen-1 (NID-1) is thought to have an important role in the construction of basement membrane by linking between the networks of laminin and collagen IV. Genetic elimination of the nidogen-1 binding site on the mouse laminin γ1 chain leads to impaired deposition of basement membranes and hampers microvessel maturation. Nidogen 1 and 2 double knockout in mouse fibroblasts led to the loss of all major basal membrane components from the epidermal-collagen interface, and complete abolishment of hemidesmosomes. Supplementation by either recombinant nidogen-1 or -2 improved basal membrane formation. Mutations in NID1and laminin γ1 is implicated in pathogenesis of Dandy-Walker spectrum disorders.
Storage Class
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Skin basement membrane: the foundation of epidermal integrity?BM functions and diverse roles of bridging molecules nidogen and perlecan
Breitkreutz D, et al.
BioMed Research International, 2013 (2013)
Analysis of nidogen-1/laminin gamma1 interaction by cross-linking, mass spectrometry, and computational modeling reveals multiple binding modes
Lossl P, et al.
Testing, 9(11), e112886-e112886 (2014)
Structural elucidation of full-length nidogen and the laminin-nidogen complex in solution
Patel TR, et al.
Matrix Biology, 33, 60-67 (2014)
Lack of nidogen-1 and-2 prevents basement membrane assembly in skin-organotypic coculture
Nischt R, et al.
The Journal of Investigative Dermatology, 127(3), 545-554 (2007)
Mutations in Extracellular Matrix Genes NID1 and LAMC1 Cause Autosomal Dominant D andy-W alker Malformation and Occipital Cephaloceles
Darbro BW, et al.
Human Mutation, 34(8), 1075-1079 (2013)
Related Content
Instructions
Global Trade Item Number
| SKU | GTIN |
|---|---|
| D8935-50UG | 04054839917141 |
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