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D9281

Lambda DNA EcoR I Hind III Digest

for DNA electrophoresis

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A ustedes/SKUDisponibilidadPrecio
0.1 mg
Comprobar disponibilidad del carrito
$288.00
0.5 mg
Comprobar disponibilidad del carrito
$1,130.00

Acerca de este artículo

NACRES:
NA.25
UNSPSC Code:
41141827
Form:
liquid
Storage temp.:
−20°C

$288.00


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Quality Segment

form

liquid

usage

100 uses (0.1 mg size), 500 uses (0.5 mg size)

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

General description

Sigma′s Lambda DNA EcoR I Hind III Digest contains 13 fragments from 125 – 21,226 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Application

Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.
Lambda DNA EcoR I Hind III Digest has been used as DNA markers for comparing the molecular weights of polymerase chain reaction (PCR) amplified products.

Preparation Note

Bacteriophage lambda DNA was digested with EcoR I and Hind III to completion, then purified by phenol extraction.

Other Notes

Contains 13 fragments, 125–21,226 bp
For optimal resolution, the recommended agarose gel concentration is 0.7%.
Sigma′s Lambda DNA EcoR I Hind III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

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Este artículo
D9780D2916D0672
description

for DNA electrophoresis

description

for DNA electrophoresis

description

for DNA electrophoresis

description

for DNA electrophoresis

Quality Level

100, 200

Quality Level

200

Quality Level

100, 200

Quality Level

100, 200

form

liquid

form

liquid

form

buffered aqueous solution

form

liquid

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

suitability

suitable for electrophoresis (DNA)

suitability

suitable for electrophoresis (DNA)

suitability

suitable for electrophoresis (DNA)

suitability

suitable for electrophoresis (DNA)

usage

100 uses (0.1 mg size), 500 uses (0.5 mg size)

usage

100 uses (0.1 mg size), 1000 uses (1 mg size), 20 uses (20 μg size), 500 uses (0.5 mg size)

usage

100 uses (0.1 mg size), 20 uses (20 μg size)

usage

200 uses (0.1 mg size), 40 uses (20 μg size)


pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1

Clase de almacenamiento

13 - Non Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



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