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JumpStart^™ Taq DNA Polymerase

Name: JumpStart<SUP>™</SUP> Taq DNA Polymerase
Brand: SIGMA
Price: 76 USD

with MgCl₂

Synonym(s):

hot start DNA polymerase, hot start PCR

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Pack Size	Availability	Price
50 units	Check Cart for Availability	$76.00
250 units	Check Cart for Availability	$274.00
1500 units	Check Cart for Availability	$1,270.00

About This Item

UNSPSC Code:

12352204

NACRES:

NA.55

MDL number:

MFCD00166026

Concentration:

2.5 units/μL

$76.00

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Properties

Quality Level

200

form

liquid

usage

sufficient for 1500 reactions, sufficient for 250 reactions, sufficient for 50 reactions

feature

dNTPs included: no, hotstart, Hot-Start PCR

concentration

2.5 units/μL

technique(s)

PCR: suitable

color

colorless

input

purified DNA

suitability

suitable for PCR

application(s)

agriculture

shipped in

wet ice

storage temp.

−20°C

Description

General description

JumpStart^™ Taq DNA polymerase is a combination of Sigma′s high-performance Taq DNA Polymerase and JumpStart Taq antibody. The Taq DNA Polymerase activity is inactivated by combining the enzyme with JumpStart Taq antibody, a neutralizing monoclonal antibody to Taq DNA polymerase. Antibody inactivation provides a simple, efficient procedure for hot-start PCR. During PCR, JumpStart Taq DNA polymerase is inactive at low (room) temperature, as the temperature is raised above 70 °C in the first denaturation step of the cycling process, the complex dissociates, and the polymerase becomes fully active.

Application

For PCR amplifications that require reduced non-specific amplification
For multiplex PCR
For reduction of primer dimers

JumpStart^™ Taq DNA Polymerase has been used:

in the amplification of DNA libraries of varying sizes[1]
in a methylation-specific, quantitative real-time polymerase chain reaction (MS-qPCR) to determine the BRCA1 promoter methylation status[2]
in the generation of plasmid by amplifying the full-length of HIF1β via PCR[3]

Features and Benefits

Reduces non-specific amplification
Increases PCR specificity and yield
Reduces set-up time concerns associated with manual or wax Hot Start methods
Activation time of less than 1 minute

Packaging

JumpStart Taq DNA Polymerase is provided with a 10× reaction buffer available with and without MgCl_2. The magnesium free 10× buffer also includes a separate tube of 25 mM MgCl₂for optimization.

Supplied with 10× reaction buffer containing 15 mM MgCl₂

Other Notes

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.

Sigma′s JumpStart Taq DNA Polymerase is an antibody-inactivated hot-start enzyme designed to minimize non-specific amplification while increasing target yield. Once the reaction temperature reaches 70°C, Taq DNA polymerase activity is restored and the resulting PCR exhibits a higher specificity and yield. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques. The enzyme may also be included in the master mix preparation resulting in more consistency from one reaction to the next.

View more detailed information on JumpStart Taq enzymes at www.sigma-aldrich.com/hotstart.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.

Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.

JumpStart is a trademark of Sigma-Aldrich Co. LLC

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Show Differences

1 of 1

This Item	D4184	D5809	D1313
Sigma-Aldrich D9307 JumpStart^™ Taq DNA Polymerase	Sigma-Aldrich D4184 JumpStart^™ Taq DNA Polymerase	Sigma-Aldrich D5809 JumpStart^™ AccuTaq^™ LA DNA Polymerase	Sigma-Aldrich D1313 JumpStart^™ REDAccuTaq^® LA DNA Polymerase
technique(s) PCR: suitable	technique(s) PCR: suitable	technique(s) PCR: suitable	technique(s) PCR: suitable
suitability suitable for PCR	suitability suitable for PCR	suitability suitable for PCR	suitability suitable for PCR
concentration 2.5 units/μL	concentration 2.5 units/μL	concentration 2.5 unit/μL	concentration 1 unit/μL
form liquid	form liquid	form liquid	form liquid
application(s) agriculture	application(s) agriculture	application(s) -	application(s) agriculture
shipped in wet ice	shipped in wet ice	shipped in wet ice	shipped in wet ice

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 3

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Articles

Hot Start PCR

The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.

High quality bisulfite sequencing using nanogram amounts of genomic DNA

Sun J, et al.

International journal of biochemistry and biotechnology, 2, 449-456 (2013)

Generation of human induced pluripotent stem cells bearing an anti-HIV transgene by a lentiviral vector carrying an internal murine leukemia virus promoter.

Masakazu Kamata et al.

Human gene therapy, 21(11), 1555-1567 (2010-06-08)

The recent development of induced pluripotent stem cells (iPSCs) by ectopic expression of defined reprogramming factors offers enormous therapeutic opportunity. To deliver these factors, murine leukemia virus (MLV)-based vectors have been broadly used in the setting of hematopoietic stem cell

Whole genome DNA methylation analysis based on high throughput sequencing technology

Li N, et al.

Methods, 52(3), 203-212 (2010)

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Global Trade Item Number

SKU	GTIN
D9307-1SET	04061833697115
D9307-50UN	04061835572847
D9307-250UN	04061835572830
D9307-1.5KU	04061835572823
MP0035-1KT	04061834066835

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