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D9655

pBR322 Hae III Digest

for DNA electrophoresis

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0.1 mg
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$931.00

About This Item

NACRES:
NA.25
UNSPSC Code:
12352202

$931.00

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Quality Level

100

form

liquid

usage

1000 uses (0.5 mg size), 200 uses (0.1 mg size)

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

General description

Sigma′s pBR322 Hae III Digest contains 22 fragments from 8 - 587 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Application

Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.

Preparation Note

Plasmid pBR322 DNA was digested with Hae III to completion, then purified by phenol extraction.

Other Notes

Contains 22 fragments, 8–587 bp
For optimal resolution, the recommended agarose gel concentration is 2.5.
Sigma′s pBR322 Hae III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

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pBR322 Hae III Digest for DNA electrophoresis

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suitability

suitable for electrophoresis (DNA)

suitability

suitable for electrophoresis (DNA)

suitability

suitable for electrophoresis (DNA)

suitability

suitable for electrophoresis (DNA)

form

liquid

form

liquid

form

liquid

form

liquid

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

100

Quality Level

100, 200

Quality Level

100, 200

Quality Level

100, 200

usage

1000 uses (0.5 mg size), 200 uses (0.1 mg size)

usage

200 uses (0.1 mg size), 40 uses (20 μg size)

usage

100 uses (0.1 mg size), 500 uses (0.5 mg size)

usage

200 uses (0.1 mg size), 40 uses (20 μg size)

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000512633
0000512633
0000451886
0000451886
0000424295

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Articles

Selecting DNA, RNA, and PCR Fragment Markers and Ladders for Gel Electrophoresis

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

Gel Electrophoresis Markers

Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.

Global Trade Item Number

SKUGTIN
D9655-.1MG04061833590959

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