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D9905

Denhardt′s Solution

for Northern and Southern blotting, lyophilized powder

Synonym(s):

Denhardt′s Solution For Blotting Technique

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Pack SizeSKUAvailabilityPrice
5 mL
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$209.00
5 x 5 mL
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$701.00

About This Item

NACRES:
NA.25
UNSPSC Code:
12161700

$209.00

Please contact Customer Service for Availability
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grade

Molecular Biology

Quality Level

100

form

lyophilized powder

technique(s)

Southern blotting: suitable

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

storage temp.

2-8°C

General description

Denhardt′s Solution is a blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. This powder can be used to make a 50x concentrate that can be diluted to any concentration for use in a variety of pre-hybridization and hybridization buffers.

Application

Suitable as a blocking Buffer for Southern and Northern blot membranes.
Denhardt′s Solution has been used:
  • as a component in hybridization buffer for the hybridization of brain sections
  • in in-situ hybridization peroxidase-(antiperoxidase method-whole mounts) (ISHPAP-WM) process
  • in the preparation of hybridization mixture[1]

Biochem/physiol Actions

Denhardt′s solution and gelatin-chrome alum can be used to treat chromosomes for G-banding with trypsin after in situ hybridization.[2]

Preparation Note

Each vial of Denhardt′s powder contains 50mg Bovine Serum Albumin (BSA), 50mg Ficoll, and 50mg Polyvinylpyrrolidone (PVP).
To make a 50x concentrate, dissolve entire contents of vial in 5 mL molecular biology grade water. Store at -20°C for up to two years.

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This Item
D2532P1415N1531
Denhardt’s Solution for Northern and Southern blotting, lyophilized powder

Sigma-Aldrich

D9905

Denhardt′s Solution

Denhardt’s Solution 50x for Northern and Southern blotting, solution

Sigma-Aldrich

D2532

Denhardt′s Solution 50x

Prehybridization Solution for Northern and Southern blotting, liquid (2x concentrate)

Sigma-Aldrich

P1415

Prehybridization Solution

Denaturation Solution for Northern and Southern blotting

Sigma-Aldrich

N1531

Denaturation Solution

grade

Molecular Biology

grade

-

grade

-

grade

Molecular Biology

technique(s)

Southern blotting: suitable

technique(s)

Southern blotting: suitable

technique(s)

-

technique(s)

-

Quality Level

100

Quality Level

300

Quality Level

100, 200

Quality Level

200

form

lyophilized powder

form

solution

form

liquid (2x concentrate)

form

solution

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

-10 to -25°C

storage temp.

room temp

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

foreign activity

-

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SLCH1455
SLCB0735
SLBW1763
SLBR5407V
SLBR7277V

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Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 9-9 (1989)
M Simpson-Holley et al.
Journal of virology, 85(4), 1581-1593 (2010-12-15)
Vaccinia virus (VV) mutants lacking the double-stranded RNA (dsRNA)-binding E3L protein (ΔE3L mutant VV) show restricted replication in most cell types, as dsRNA produced by VV activates protein kinase R (PKR), leading to eIF2α phosphorylation and impaired translation initiation. Here
E J Mackie et al.
Journal of cell science, 103 ( Pt 3), 765-771 (1992-11-01)
The extracellular matrix glycoprotein, tenascin, is associated in vivo with mesenchyme undergoing osteogenesis and chondrogenesis, but is absent from mature bone and cartilage matrix. The expression of tenascin by osteoblastic cells in vitro has been investigated by immunoblotting and immunocytochemistry.
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Global Trade Item Number

SKUGTIN
D9905-5X5ML04061833591345
D9905-5ML04061833591338

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