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D9909

Dextran Sucrase from Leuconostoc mesenteroides

lyophilized powder, ≥100 units/mg protein

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10 units

Estimated to ship onMay 21, 2026fromMILWAUKEE

$488.00

About This Item

CAS Number:
9032-14-8
EC Number:
2.4.1.5 (BRENDA, IUBMB)
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
MFCD00130936
Specific activity:
≥100 units/mg protein
Biological source:
bacterial (Leuconostoc mesenteroides)

$488.00

Estimated to ship onMay 21, 2026Details

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biological source

bacterial (Leuconostoc mesenteroides)

Quality Level

200

form

lyophilized powder

specific activity

≥100 units/mg protein

composition

Protein, ~15% Lowry

solubility

H2O: soluble 0.9-1.1 mg/mL, clear to slightly hazy, colorless to light yellow

storage temp.

−20°C

General description

Dextran Sucrase from Leuconostoc mesenteroides belongs to glycoside hydrolase family 70 (GH70). It functions through a retaining mechanism and uses two catalytic acidic residues. Dextran sucrase has a dextran binding site in the C-terminal domain.

Application

Dextran sucrase from Leuconostoc mesenteroides has been used in a study to investigate the functional and structural characterization of α-(1→2) branching sucrase derived from DSR-E glucansucrase. Dextran sucrase from Leuconostoc mesenteroides has also been used in a study to investigate the bioengineering of Leuconostoc mesenteroides glucansucrases.
Dextran Sucrase from Leuconostoc mesenteroides has been used:
  • in immobilization on shirasu porous membrane (SPG) for dextran production
  • in the enzymatic synthesis of dextran nanoparticles at various pH range
  • in the immobilization with hydroxyapatite for dextran production

The enzyme from Sigma has been used to prepare immobilized sphere for the production of dextran from sucrose.

Biochem/physiol Actions

Dextransucrases are glucansucrases that are able to produce dextran, a glucose polymer linked mainly through α1-6 bonds. However, α1-3, α1-6, α1-4 and α1-2 bonds are also found, in both the main chain and the branching linkages.[1] The peptide has approximately 1600 amino acids. The aspartic acid in position 551 is essential for catalytic activity, while glutamic acid 589 and aspartic acid 662 complement the catalytic triad.[2] The activity of dextransucrase is decreased by EDTA, and is restored by the addition of calcium ions. Zinc, cadmium, lead, mercury and copper ions are inhibitory to various degrees.[3]

Physical form

Lyophilized powder containing dextran, MES buffer salts and CaCl2

Analysis Note

Chromatographically purified

Other Notes

One unit will liberate 1.0 μmole of fructose per min at 37 °C, pH 5.4.

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Dextran Sucrase from Leuconostoc mesenteroides lyophilized powder, ≥100 units/mg protein

Sigma-Aldrich

D9909

Dextran Sucrase from Leuconostoc mesenteroides

Dextran from Leuconostoc mesenteroides average mol wt 150,000

Sigma-Aldrich

D4876

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Dextran from Leuconostoc mesenteroides average mol wt 35,000-45,000

Sigma-Aldrich

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Dextran from Leuconostoc mesenteroides average mol wt 48,000-90,000

Sigma-Aldrich

D3759

Dextran from Leuconostoc mesenteroides

biological source

bacterial (Leuconostoc mesenteroides)

biological source

bacterial (Leuconostoc mesenteroides)

biological source

bacterial (Leuconostoc mesenteroides)

biological source

bacterial (Leuconostoc mesenteroides)

specific activity

≥100 units/mg protein

specific activity

-

specific activity

-

specific activity

-

form

lyophilized powder

form

powder

form

powder

form

powder

storage temp.

−20°C

storage temp.

-

storage temp.

-

storage temp.

-

solubility

H2O: soluble 0.9-1.1 mg/mL, clear to slightly hazy, colorless to light yellow

solubility

H2O: soluble 20 mg/mL

solubility

H2O: 100 mg/mL, clear, colorless

solubility

H2O: 50 mg/mL, clear to very slightly hazy, colorless to faintly yellow

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000216677
0000216677
0000142157
0000138732
11140606

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Pore control with dextran generated from immobilized dextransucrase
Kawakita H, et al.
Biochemical Engineering Journal, 36(2), 190-193 (2007)
J F Robyt et al.
Carbohydrate research, 68(1), 95-111 (1979-01-01)
The production of dextransucrase from Leuconostoc mesenteroides NRRL B-512F was stimulated 2-fold by the addition of 0.005% of calcium chloride to the medium; levansucrase levels were unaffected. Dextransucrase was purified by concentration and dialysis of the culture supernatant with a
S A Ul-Qader et al.
Biotechnology and applied biochemistry, 34(Pt 2), 93-97 (2001-10-11)
A newly isolated strain of Leuconostoc mesenteroides (PCSIR-3) produced a different dextran compared with that of L. mesenteroides NRRL B-512F. Different media compositions used for dextran production showed that media containing CaCl(2) produced dextran in higher quantities compared with other
View All Related Papers

Global Trade Item Number

SKUGTIN
D9909-10UN04061833591352

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