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Anti-dEGF Receptor, Extracellular Domain antibody, Mouse monoclonal

Name: Anti-dEGF Receptor, Extracellular Domain antibody, Mouse monoclonal
Brand: SIGMA
Price: 526.15 CHF

clone C-273, purified from hybridoma cell culture

Synonym(s):

Anti-Drosophila Epidermal Growth Factor Receptor, Anti-dEGFR

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Size/SKU	Availability	Price
200 μL	Check Cart for Availability	CHF 619.00 CHF 526.15

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD08705583

Conjugate:

unconjugated

Clone:

C-273, monoclonal

Application:

ELISA (i), IHC, WB

Citations:

CHF 526.15

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

C-273, monoclonal

form

buffered aqueous solution

species reactivity

Drosophila

concentration

~1.2 mg/mL

technique(s)

immunohistochemistry: suitable, indirect ELISA: suitable, western blot: 0.25-0.5 μg/mL using recombinant protein representing amino acids 299-359 of the Drosophila EGFR extracellular domain

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

Drosophila melanogaster ... Egfr(37455)

Description

General description

Anti-dEGF Receptor, Extracellular Domain antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma C-273 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a recombinant protein encoding at the extracellular portion of Drosophila epidermal growth factor receptor (EGFR). Drosophila EGF receptor, also known as Torpedo or DER has four ligands: Gurken, Spitz (the principle ligand), Vein and Argos. Two accessory proteins modulate its signalling: Rhomboid and Star.

Application

Suitable for the following:

Immunohistochemistry
Indirect ELISA
Western blotting at a concentration of 0.25-0.5μg/mL using recombinant protein representing amino acids 299-359 of the Drosophila EGFR extracellular domain

Biochem/physiol Actions

Drosophila epidermal growth factor receptor (EGFR) is involved in many developmental processes such as: egg polarity, cell identity in the ventral ectoderm, neurogenesis, development of the Malpighian tubules, and larval eye and wing development. The downstream signalling molecules that are activated by the Drosophila EGF receptor include: Src homology (Shc), DRK (a homolog of mammalian Grb2), a guanine nucleotide exchange factor (SOS) activated by DRK, and downstream targets including orthologs of Ras, Raf, and Rolled (MAP Kinase).

The epidermal growth factor receptor (EGFR) binds to its cognate ligands that lead to autophosphorylation of receptor tyrosine kinase and subsequent activation of signal transduction pathways that are involved in regulating cellular proliferation, differentiation and survival. EGFR is overexpressed in tumor cell lines and is associated with poor prognosis leading to decreased survival. Its activation also plays a role in resistance to chemotherapy and radiation treatment in tumor cells. EGFR tyrosine kinase inhibitors (TKIs) may act as a new treatment option for advanced lung adenocarcinoma. Its ligands function in the Drosophila CNS and maintains glial survival. Drosophila EGF receptor plays a critical role during the development of Drosophila retina.

Physical form

Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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1 of 1

This Item	E2156	E2760	E2520
Sigma-Aldrich E2906 Anti-dEGF Receptor, Extracellular Domain antibody, Mouse monoclonal	Sigma-Aldrich E2156 Anti-EGF Receptor antibody, Mouse monoclonal	Sigma-Aldrich E2760 Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse	Sigma-Aldrich E2520 Anti-Epidermal Growth Factor antibody, Mouse monoclonal
biological source mouse	biological source mouse	biological source mouse	biological source mouse
clone C-273, monoclonal	clone 225, monoclonal	clone 29.1, monoclonal	clone EGF-10, monoclonal
antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form ascites fluid	antibody form purified from hybridoma cell culture
Gene Information Drosophila melanogaster ... Egfr(37455)	Gene Information human ... EGFR(1956)	Gene Information human ... EGFR(1956) mouse ... Egfr(13649) rat ... Egfr(24329)	Gene Information human ... EGF(1950)
species reactivity Drosophila	species reactivity human	species reactivity pig, human, rat, mouse, canine	species reactivity human
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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