E2906
Anti-dEGF Receptor, Extracellular Domain antibody, Mouse monoclonal
clone C-273, purified from hybridoma cell culture
Synonym(s):
Anti-Drosophila Epidermal Growth Factor Receptor, Anti-dEGFR
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 200 μL | Available to ship TODAYfromMILWAUKEE | $730.00 $620.50 |
About This Item
Conjugate:
unconjugated
Clone:
C-273, monoclonal
Application:
ELISA (i), IHC, WB
Citations:
15
$620.50
List Price$730.00Save 15%Web-Only Promotion
Available to ship TODAYDetails
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
C-273, monoclonal
form
buffered aqueous solution
species reactivity
Drosophila
concentration
~1.2 mg/mL
technique(s)
immunohistochemistry: suitable, indirect ELISA: suitable, western blot: 0.25-0.5 μg/mL using recombinant protein representing amino acids 299-359 of the Drosophila EGFR extracellular domain
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
Drosophila melanogaster ... Egfr(37455)
General description
Anti-dEGF Receptor, Extracellular Domain antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma C-273 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a recombinant protein encoding at the extracellular portion of Drosophila epidermal growth factor receptor (EGFR). Drosophila EGF receptor, also known as Torpedo or DER has four ligands: Gurken, Spitz (the principle ligand), Vein and Argos. Two accessory proteins modulate its signalling: Rhomboid and Star.
Application
Suitable for the following:
- Immunohistochemistry
- Indirect ELISA
- Western blotting at a concentration of 0.25-0.5μg/mL using recombinant protein representing amino acids 299-359 of the Drosophila EGFR extracellular domain
Biochem/physiol Actions
Drosophila epidermal growth factor receptor (EGFR) is involved in many developmental processes such as: egg polarity, cell identity in the ventral ectoderm, neurogenesis, development of the Malpighian tubules, and larval eye and wing development. The downstream signalling molecules that are activated by the Drosophila EGF receptor include: Src homology (Shc), DRK (a homolog of mammalian Grb2), a guanine nucleotide exchange factor (SOS) activated by DRK, and downstream targets including orthologs of Ras, Raf, and Rolled (MAP Kinase).
The epidermal growth factor receptor (EGFR) binds to its cognate ligands that lead to autophosphorylation of receptor tyrosine kinase and subsequent activation of signal transduction pathways that are involved in regulating cellular proliferation, differentiation and survival. EGFR is overexpressed in tumor cell lines and is associated with poor prognosis leading to decreased survival. Its activation also plays a role in resistance to chemotherapy and radiation treatment in tumor cells. EGFR tyrosine kinase inhibitors (TKIs) may act as a new treatment option for advanced lung adenocarcinoma. Its ligands function in the Drosophila CNS and maintains glial survival. Drosophila EGF receptor plays a critical role during the development of Drosophila retina.
Physical form
Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
1 of 1
This Item | |||
|---|---|---|---|
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| Gene Information Drosophila melanogaster ... Egfr(37455) | Gene Information human ... EGFR(1956) | Gene Information human ... EGF(1950) | Gene Information human ... EGFR(1956) |
| antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form purified from hybridoma cell culture | antibody form ascites fluid |
| clone C-273, monoclonal | clone 225, monoclonal | clone EGF-10, monoclonal | clone F4, monoclonal |
| species reactivity Drosophila | species reactivity human | species reactivity human | species reactivity human |
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Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Related Content
Instructions
Regulating the dynamics of EGF receptor signaling in space and time
Shilo BZ
Development, 132(18), 4017-4027 (2005)
Josefa Steinhauer et al.
Journal of cell science, 126(Pt 19), 4469-4478 (2013-08-02)
Epidermal growth factor receptor (EGFR) ligands undergo a complex series of processing events during their maturation to active signaling proteins. Like its mammalian homologs, the predominant Drosophila EGFR ligand Spitz is produced as a transmembrane pro-protein. In the secretory pathway
Rebecca P Kim-Yip et al.
Development (Cambridge, England), 145(23) (2018-11-06)
Adult stem cell niche boundaries must be precisely maintained to facilitate the segregation of stem cell and daughter cell fates. However, the mechanisms that govern this process in epithelial tissues are not fully understood. In this study, we investigated the
Global Trade Item Number
| SKU | GTIN |
|---|---|
| E2906-200UL | 04061837794094 |